Introduction Stromal Vascular Fraction cells (SVFs) and Platelet Rich Plasma (PRP) are clinically proven to aid in cell regeneration and wound healing. The healing effects can be measured by the level of Epidermal Growth Factor (EGF). This study aims to investigate the effect of an SVFs and PRP combination on EGF levels in the anal trauma model. Method Twenty-eight adult Wistar rats were divided into 3 groups: Group A consisted of healthy rats as a normal control group; Group B and C underwent modified anal surgical trauma and repair. Group B was treated with saline only and Group C was treated with local injection of a combination of SVFs and PRP after anal surgical repair. The EGF level was subsequently assessed on days 1, 7, and 14. Results EGF levels were generally increased in Group C compared to Group B. A one-way ANOVA test result showed significantly increased EGF levels on days 7 (p = 0.038) and 14 (p = 0.018). Based on the linear regression test results, we found that local injection of PRP and SVFs after anal repair on an anal surgical trauma model can increase the EGF level in group C by 36.9% more than that of group B. Conclusion The combination of PRP and SVFs can increase the EGF level in the wound healing process of anal trauma. EGF is critical in the anal trauma healing process.

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Histopathological changes that are usually permanent in the bowel of Hirschsprung's disease (HRSC) in children make susceptibility to the incidence of enterocolitis even after definitive therapy. The pathophysiology of HRSC is characterized by specific signs, and symptoms. Scoring system has been applied in diagnosis of Hirschsprung's associated enterocolitis (HAEC) due to its simplest and easiest. However, after a decade it has been several evaluated to obtain optimal benefits in the clinical setting. The aim of this study was to investigate the relationship between histopathological grade of enterocolitis and HAEC scores. Children with HRSC who underwent leveling colostomy or pull-through were sampled from the ganglionic segment. Those with a histopathological description of HAEC were included in the study. HAEC scores were evaluated retrospectively through medical records and interviews. Data were analyzed using the Spearman's Rank test. Twenty eight samples were involved in this study. The population was dominated by male (82.1%) and almost half the population (42.9%) were infants. The distribution of the histopathological grade was fairly even, except for grade V where it was only 7.2% of the total sample. Grade I & III samples were 21.4%, while grade II & IV were 25%, respectively. A significant relationship between histopathological grade and HAEC score was obtained (r = 0.927; p<0.0001). In conclusion, there is a relationship between the histopathological grade of enterocolitis and HAEC scores.

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