Human acellular amniotic membrane scaffolds encapsulating juvenile cartilage fragments accelerate the repair of rabbit osteochondral defects

Zhang, Jun; Wang, Yuping; Huang, Yanran; Liu, Zi-Ming; Li, Yuwan; Zhu, Xiaodong; Wu, Zhongliang; Luο, Xingguang

doi:10.1302/2046-3758.116.bjr-2021-0490.r1

Cited by 5 publications

(7 citation statements)

References 60 publications

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“…A recent study by Zhang et al demonstrated the feasibility of human acellular amniotic membrane (HAAM) scaffolds (Figure 3B). [38] They seeded third-generation (P3) human amniotic mesenchymal stem cells and juvenile cartilage fragments (JCFs) on it and placed the scaffolds in rabbits with osteochondral defects. The results showed that the HAAM scaffolds combined with JCFs promoted the repair of cartilage defects.…”

Section: Collagen and Gelatinmentioning

confidence: 99%

“…[ 26 ] Copyright 2021, Elsevier Inc. B) Schematic diagram of the fabrication of human acellular amniotic membrane scaffolds. [ 38 ] Reproduced with permission. [ 38 ] Copyright 2022, Bone & Joint Research.…”

Section: Terrestrial‐inspired Materials For Oamentioning

confidence: 99%

Section: Terrestrial‐inspired Materials For Oamentioning

confidence: 99%

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Nature‐Inspired Strategies for the Treatment of Osteoarthritis

Wu,

Zheng,

et al. 2023

Adv Funct Materials

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Articular cartilage is devoid of nerves and blood vessels, and its nutrients must be obtained from the joint fluid; therefore, its ability to repair itself is limited. Manufactured materials such as artificial cartilage or synthetic materials are typically used in traditional approaches for knee cartilage repair. However, durability, postimplant rejection, and tissue incompatibility are the problems associated with these materials. In recent decades, tissue engineering and regenerative medicine have focused on the development of functional substitutes, particularly those based on naturally inspired biopolymers. This review focuses on sustainably produced biopolymers based on materials derived from natural sources. Furthermore, these materials have many advantages, including low antigenicity, biocompatibility, and degradability. Of course, there are also many challenges associated with natural materials, such as the lack of clinical studies and long‐term follow‐up data, unstable mechanical properties of the materials, and high demands placed on preparation and molding techniques. In this review, an overview of natural and nature‐inspired polymers that are the subject of research to date, as well as their structural designs and product performances is provided. This review provides scientific guidance for enhancing the development of naturally inspired materials for treating cartilage injuries.

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Section: Collagen and Gelatinmentioning

confidence: 99%

Section: Terrestrial‐inspired Materials For Oamentioning

confidence: 99%

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Nature‐Inspired Strategies for the Treatment of Osteoarthritis

Wu,

Zheng,

et al. 2023

Adv Funct Materials

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“…Several matrix-induced chondrogenic techniques have recently been developed and have demonstrated acceptable clinical results for medium-sized lesions. [5][6][7][8] These methods are potential strategies for treating medium-sized lesions via a single-step procedure. Alginate gels are biocompatible materials used to replace chondral defects and have demonstrated biochemical characteristics similar to native hyaline cartilage.…”

Section: Introductionmentioning

confidence: 99%

Single-step ultra-purified alginate gel implantation in patients with knee chondral defects

Onodera,

Momma,

Matsuoka

et al. 2023

Bone Joint J

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AimsImplantation of ultra-purified alginate (UPAL) gel is safe and effective in animal osteochondral defect models. This study aimed to examine the applicability of UPAL gel implantation to acellular therapy in humans with cartilage injury.MethodsA total of 12 patients (12 knees) with symptomatic, post-traumatic, full-thickness cartilage lesions (1.0 to 4.0 cm2) were included in this study. UPAL gel was implanted into chondral defects after performing bone marrow stimulation technique, and assessed for up to three years postoperatively. The primary outcomes were the feasibility and safety of the procedure. The secondary outcomes were self-assessed clinical scores, arthroscopic scores, tissue biopsies, and MRI-based estimations.ResultsNo obvious adverse events related to UPAL gel implantation were observed. Self-assessed clinical scores, including pain, symptoms, activities of daily living, sports activity, and quality of life, were improved significantly at three years after surgery. Defect filling was confirmed using second-look arthroscopy at 72 weeks. Significantly improved MRI scores were observed from 12 to 144 weeks postoperatively. Histological examination of biopsy specimens obtained at 72 weeks after implantation revealed an extracellular matrix rich in glycosaminoglycan and type II collagen in the reparative tissue. Histological assessment yielded a mean overall International Cartilage Regeneration & Joint Preservation Society II score of 69.1 points (SD 10.4; 50 to 80).ConclusionThis study provides evidence supporting the safety of acellular UPAL gel implantation in facilitating cartilage repair. Despite being a single-arm study, it demonstrated the efficacy of UPAL gel implantation, suggesting it is an easy-to-use, one-step method of cartilage tissue repair circumventing the need to harvest donor cells.Cite this article: Bone Joint J 2023;105-B(8):880–887.

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“…AAM has been shown in some tests to lessen acute liver injury and speed bone repair. (Jun et al ., 2022).…”

Section: Introductionmentioning

confidence: 99%

Assessment of serum alkaline phosphatase and calcium levels during bone fracture healing in dogs stabilized with a bone plate after being treated with an amniotic membrane loaded with platelet rich plasma

Ghadban¹,

Khashjoori²

2022

Journal of Pharmaceutical Negative Results

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Background/aim: The goal of the present study was to investigate the effects of an acellular amniotic membrane loaded with autologous platelet-rich plasma on bone healing biochemically by measuring serum factors including calcium and alkalinity in a dog model. Materials and methods: Fifteen dogs were used in the current study. The animals were divided into three groups of five dogs each. A transverse osteotomy was induced in the midshaft of the left tibia of all animals. The first group was treated with acellular amniotic membrane only by wrapping it around the fracture site. The second group was given acellular amniotic membrane and platelet-rich plasma to treat them, while the third group (the control) was left alone and not treatedWhole blood was drawn from all groups on the 0th day prior to surgery as well as on the 14th, 28th, 42nd, and 56th days following surgery. Physiological data was then reported. in order to estimate calcium and alkaline phosphatase at various phases of fracture healing. Results: the results of the present study illustrated that the serum alkaline phosphate and calcium levels increased (p≤0.01) on days 14 and 28, followed by a decreasing trend after day 28 to day 56. And their levels were higher in group AM+PRP than in other groups (39.4 ± 1.21, 15.79±0.66 respectively). When compared to the control and AM groups, the rate of formation of new bone tissue in Group AM+PRP was the fastest. It was concluded that serum alkaline phosphatase and calcium were found to be helpful biochemical indicators for evaluating bone development and contributed to proving the superiority of using the amniotic membrane loaded with platelet-rich plasma in promoting bone healing. These biochemical parameters, in conjunction with clinical and radiographic examinations, provide reliable information about the degree of bone healing.

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Human acellular amniotic membrane scaffolds encapsulating juvenile cartilage fragments accelerate the repair of rabbit osteochondral defects

Cited by 5 publications

References 60 publications

Nature‐Inspired Strategies for the Treatment of Osteoarthritis

Nature‐Inspired Strategies for the Treatment of Osteoarthritis

Single-step ultra-purified alginate gel implantation in patients with knee chondral defects

Assessment of serum alkaline phosphatase and calcium levels during bone fracture healing in dogs stabilized with a bone plate after being treated with an amniotic membrane loaded with platelet rich plasma

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