Evaluation of seminal citrate level by 1H nuclear magnetic resonance spectroscopy in men with spinal cord injury

Alexandrino, Antonio Paulo; Rodrigues, Marco Aurélio Freitas; Matsuo, Tiemi; Schuquel, Ivânia Terezinha Albrecht; Costa, Willian Ferreira da; Santilli, J C

doi:10.1038/sc.2009.62

Cited by 6 publications

(3 citation statements)

References 18 publications

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“…The normal value of the citrate concentration in the seminal fluid ([CITRATE]) depends on the methodology used for measurement. A study (19) that evaluated 30 healthy young men (mean age 38.03±10.06 years), using a methodology identical to that of the current study to measure [CITRATE], found a median of [CITRATE] in these men of 44.68mM/l. In the current study, median [CITRATE] was 3.95-fold higher in controls with BPH than in patients with PCa (15.53 vs. 3.93mM/l, p=0.0011).…”

Section: Discussionmentioning

confidence: 61%

Seminal citrate is superior to PSA for detecting clinically significant prostate cancer

et al. 2019

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Purpose: To establish whether the citrate concentration in the seminal fl uid ([CI-TRATE]) measured by means of high-resolution nuclear magnetic resonance spectroscopy (1HNMRS) is superior to the serum prostate-specifi c antigen (PSA) concentration in detecting of clinically signifi cant prostate cancer (csPCa) in men with persistently elevated PSA. Materials and Methods: The group of patients consisted of 31 consecutively seen men with histological diagnosis of clinically localized csPCa. The control group consisted of 28 men under long-term follow-up (mean of 8.7 ± 3.0 years) for benign prostate hyperplasia (BPH), with persistently elevated PSA (above 4 ng/mL) and several prostate biopsies negative for cancer (mean of 2.7 ± 1.3 biopsies per control). Samples of blood and seminal fl uid (by masturbation) for measurement of PSA and citrate concentration, respectively, were collected from patients and controls. Citrate concentration in the seminal fl uid ([CITRATE]) was determined by means of 1HNMRS. The capacities of PSA and [CITRATE] to predict csPCa were compared by means of univariate analysis and receiver operating characteristic (ROC) curves. Results: Median [CITRATE] was signifi cantly lower among patients with csPCa compared to controls (3.93 mM/l vs. 15.53 mM/l). There was no signifi cant difference in mean PSA between patients and controls (9.42 ng/mL vs. 8.57 ng/mL). The accuracy of [CITRATE] for detecting csPCa was signifi cantly superior compared to PSA (74.8% vs. 54.8%). Conclusion: Measurement of [CITRATE] by means of 1HNMRS is superior to PSA for early detection of csPCa in men with elevated PSA.

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Section: Discussionmentioning

confidence: 61%

Seminal citrate is superior to PSA for detecting clinically significant prostate cancer

et al. 2019

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“…In fact, citrate is the unique prostatic metabolite in seminal plasma investigated by high resolution NMR analysis as a marker to diagnose prostatic cancer [Averna et al 2005] or in men with spinal cord injury [Alexandrino et al 2009]. None of these conditions were detected in our patient population.…”

Section: Resultsmentioning

confidence: 85%

Discrimination of human semen specimens by NMR data, sperm parameters, and statistical analysis

Bonechi

Collodel

Donati

et al. 2015

Systems Biology in Reproductive Medicine

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Human seminal fluid is a complex mixture of secretions originated from epididymis and the male accessory sex glands. It contains a variety of both inorganic and organic components, among which proteins are a major part of the high molecular-mass substances. In this study, 83 human seminal plasma samples were analyzed using a combined Nuclear Magnetic Resonance (NMR) Spectroscopy and Principal Component Analysis (PCA) approach to discriminate patients in relation to semen characteristics and/or conditions affecting the fertility status. Results showed a discrimination between patients with leukocytospermia and with the concomitant presence of varicocele/ex varicocele and leukocytospermia. Patients with testicular cancer, necrozoospermia, and azoospermia were separated from the other patient clusters. In addition, a differentiation of semen quality was also possible. This study represents to first use of sperm parameters together with NMR data as variables in the PCA analysis. Furthermore, this methodology allows the identification of the metabolites which play the most important role in identifying differences among human seminal plasma samples.

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“…The utility of 1 H NMR spectroscopy to highlight changes in the seminal plasma of infertile men with spinal cord injury (SCI) due to neurological dysfunction of the prostate has been demonstrated [3,4]. The presence of higher peptidase activity in infertile men with SCI and that uridine is likely to be an essential precursor to metabolites required for capacitation and is a potential marker for the prognosis of functional fertility recovery in patients with SCI has now been established [24,23].…”

Section: Discussionmentioning

confidence: 99%

Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy

Jayaraman

Ghosh

Sengupta

et al. 2014

J Assist Reprod Genet

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Purpose The aim of this study was to analyze the seminal plasma of patients with idiopathic/male factor infertility and healthy controls with proven fertility by NMR spectroscopy, with a hope of establishing difference in biomarker profiles, if any, between the groups. Methods A total of 103 subjects visiting the infertility clinic of Manipal University with normozoospermic parameters, oligozoospermia, asthenozoospermia, azoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria. Seminal plasma was subjected to NMR spectroscopy at a 700 MHz

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Evaluation of seminal citrate level by 1H nuclear magnetic resonance spectroscopy in men with spinal cord injury

Abstract: Patients with SCI have a significant reduction of seminal citrate as a consequence of neurological dysfunction of the prostate.

Cited by 6 publications

References 18 publications

Seminal citrate is superior to PSA for detecting clinically significant prostate cancer

Seminal citrate is superior to PSA for detecting clinically significant prostate cancer

Discrimination of human semen specimens by NMR data, sperm parameters, and statistical analysis

Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy

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