Sperm preparation has been found to result in enrichment of sperm with intact chromatin, which is likely to improve the chances of achieving a viable pregnancy.

Purpose The aim of this study was to analyze the seminal plasma of patients with idiopathic/male factor infertility and healthy controls with proven fertility by NMR spectroscopy, with a hope of establishing difference in biomarker profiles, if any, between the groups. Methods A total of 103 subjects visiting the infertility clinic of Manipal University with normozoospermic parameters, oligozoospermia, asthenozoospermia, azoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria. Seminal plasma was subjected to NMR spectroscopy at a 700 MHz

Objective: The aim of this experimental prospective study was to investigate the efficacy of single and combination sperm wash methods for their ability to isolate DNA intact spermatozoa. Material and Methods:Sperm DNA damage was introduced by local testicular irradiation in male mice and the extent of damage was quantified by comet assay. The spermatozoa were subjected to single (swim up or density gradient method) and also a combination of sperm wash techniques. The DNA integrity in various sub-fractions of wash techniques was evaluated. Results:The amount of DNA damaged sperm did not differ between individual fractions when single wash technique was applied. However, a combination of density gradient and swim-up techniques significantly reduced (p<0.01) the number of DNA damaged sperm in the final population. Conclusion:The combination of density gradient separation and swim-up method is effective in eliminating DNA damaged spermatozoa. (J Turkish-German Gynecol Assoc 2011; 12: 148-52) Key words: Sperm wash, DNA damage, swim-up, density gradient separation, comet assay Received: 13 June, 2011 Accepted: 19 July, 2011 Amaç: Bu deneysel prospektif çalışmanın amacı tek ve kombinasyon sperm yıkama metotlarının, DNA'sı sağlam spermatozoa izolasyon etkinliklerini araştırmaktı. Gereç ve Yöntemler:Sperm DNA hasarı erkek farelerde lokal testiküler ışınlama ile oluşturuldu ve hasarın büyüklüğü comet assay ile öl-çüldü. Spermatozoa'ya tek (yüzdürme veya dansite gradient metodu) ve ayrıca kombinasyon sperm yıkama teknikleri uygulandı. Yıkama tekniklerinin çeşitli alt fraksiyonlarında DNA'nın bütünlüğü değerlen-dirildi. Bulgular:Tek yıkama tekniği uygulandığında DNA hasarlı sperm miktarı fraksiyonlar arasında farklılık göstermedi. Bununla beraber, dansite gradienti ve yüzdürme tekniklerinin kombinasyonu son popü-lasyondaki DNA hasarlı sperm sayısını anlamlı olarak (p<0.01) azalttı.Sonuç: Dansite gradient ayırma ve yüzdürme mettotlarının kombinasyonu DNA hasarlı spermatozoa'nın uzaklaştırılmasında etkilidir.