Evaluation of semen quality of buffalo frozen semen produced by Artificial Insemination Center

Mahendra, H. C.; Samsudewa, Daud; Ondho, Yon Soepri

doi:10.14710/jitaa.43.1.26-34

Cited by 5 publications

(7 citation statements)

References 10 publications

(10 reference statements)

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“…However, the percentage of sperm IA of bulls with double stain is around 34%-39% [33]. A sperm acrosome intactness has a significant effect on FSCR and contributes to determining the fertility rate of the bull [34]. Damage to the plasma membrane and acrosome membrane of sperm will lower the potential of sperm fertility [35] (Table -3).…”

Section: Discussion the Post-freeze Thawing Quality Of Holstein Bull Sperm And The Correlation With Fertility Levelmentioning

confidence: 99%

Sperm protein markers for Holstein bull fertility at National Artificial Insemination Centers in Indonesia

et al. 2020

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Background and Aim: Holstein cows and heifers are widely bred in Indonesia by artificial insemination (AI) to increase population and milk production. Sperm fertility is modulated by genetic factors, but the analysis of sperm quality is still based on macro- and microscopic characteristics. This study aimed to analyze both sperm quality and proteins of Holstein bulls at different fertility levels. Materials and Methods: The frozen semen samples were collected from the Indonesia National AI Center. They were classified based on the reproductive efficiency data and were grouped into high fertile (HF) and low fertile (LF). Sperm qualities were evaluated by microscopic evaluation. The Holstein sperm proteins were extracted using phenylmethanesulfonyl fluoride as a protease inhibitor and the benzidine detergent extraction method. Discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was conducted to analyze the molecular weights (MWs) of the sperm proteins. The data obtained were analyzed by a t-test using the one-factor bull fertility level, and Spearman's correlation analysis was used to identify the correlation between the sperm microscopic evaluation parameters and protein bands. Results: The sperm motility post-freeze thawing was not significantly different between the HF and LF (p>0.05). The HF level had a higher percentage of viability, intact plasma membrane integrity, and intact acrosomes than the LF (p<0.05). Five protein bands were found in the SDS-PAGE of sperm proteins of Holstein bulls with different concentrations. Sperm proteins with MWs of 17.51 kDa, 14.87 kDa, 33.71 kDa, and 41.97 kDa were abundant in the Holstein bulls with an HF level, while 55 kDa proteins were abundant in the LF level of Holstein bulls. The sperm of Holstein bulls in the HF level contained proteins of about 33.71 kDa that were not detected in the LF. Conclusion: The sperm protein with a molecular weight of 33.71 kDa was predicted to be a specific protein biomarker that influences bull fertility. Sperm fertilization abilities were also determined by the sperm proteins, the morphology of sperm acrosomes, and the quality of plasma membranes. This method can be used to select bulls with high fertility to increase the population of Holstein bulls.

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Section: Discussion the Post-freeze Thawing Quality Of Holstein Bull Sperm And The Correlation With Fertility Levelmentioning

confidence: 99%

Sperm protein markers for Holstein bull fertility at National Artificial Insemination Centers in Indonesia

et al. 2020

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“…: Correction factor because only 5 out of 25 squares are counted FP : Dilution factor (1:100) 10.000 : Depth of the Neubauer chamber (0.0001 ml/Neubauer chamber) The total motile spermatozoa can be calculated by multiplying the percentage of individual sperm motility by the sperm concentration and the semen volume [7].…”

Section: Assessment Of Sperm Quality After Sexingmentioning

confidence: 99%

Quality and Proportion of Spermatozoa in the Percoll Density Gradient Centrifugation Sexing Method at Different Gradients using AndroMed® Diluent

Simbolon,

Pramudhita,

Rizqianti

et al. 2024

BIO Web Conf.

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The utilization of sexing technology is an effort made to improve the efficiency of livestock farming, which was created to predict the sex of the calves born so that it can be adjusted to the objectives of the farm. This study aims to analyze the quality and proportion of sexed spermatozoa using the percoll density centrifugation method at different gradients with AndroMed® diluent. The research material used was fresh semen from Belgian Blue Bull aged six years with a body weight of 600 kg and fresh semen quality motility ≥70%. This research is a laboratory experiment with two treatments and ten replicates. The treatments in the study were T0: Ten Gradient density percoll + AndroMed® and T1: Five Gradient density percoll + AndroMed®. The research analysis used a dependent t-test. The statistical analysis showed a significant difference (P<0.05) in treating ten gradients with five gradients in each layer regarding individual motility, viability, abnormality, and total motile spermatozoa. At the same time, in concentration, there was no difference (P>0.05) in the treatment of 10 gradients with five gradients. The average results on individual motility, abnormality, concentration, and total motile spermatozoa showed treatment of 10 gradients better than treatment of 5 gradients in each layer. At the same time, the variable viability showed that the gradient treatment is better than the five-gradient treatment. Proportion of spermatozoa in the Upper Layer T0 X (23.4%), Y (76.8%) and T1 X (16.9%), Y (83.1%). The proportion of Spermatozoa in the Bottom Layer was T0 X (84.1%), Y (15.9%), and T1 X (83%), Y (17%), respectively. In conclusion, sexing spermatozoa with the Percoll 10 and 5 Gradient Density Centrifugation method can separate X and Y spermatozoa.

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“…The percentage of abnormality is calculated using the formula: sPermAtozoA ConCentrAtion Spermatozoa concentration was calculated using the Neubauer chamber. The concentration test procedure follows the method (Mahendra et al, 2018;Aldini et al, 2022).…”

Section: Abnormal Spermatozoamentioning

confidence: 99%

The Quality and Proportion of Spermatozoa X and Y in Sexed Frozen Semen Separated with Percoll Density Gradient Centrifugation Method on Friesian Holstein Bull

Yekti¹,

Rahayu²,

Ciptadi³

et al. 2023

AAVS

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Furthermore, the sexing process also reduces the concentration of spermatozoa, including the sexing process using the percoll method. It is because there is a complex process starting from separation through gradient levels, centrifugation, dilution, and freezing. The results of previous studies showed that the concentration of percoll sexing results in frozen Limousine Y cattle semen was 12.125 million/straw (Mahfud et al., 2019) and did not meet the Indonesian national standard of 25 million/ straw.

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Evaluation of semen quality of buffalo frozen semen produced by Artificial Insemination Center

Cited by 5 publications

References 10 publications

Sperm protein markers for Holstein bull fertility at National Artificial Insemination Centers in Indonesia

Sperm protein markers for Holstein bull fertility at National Artificial Insemination Centers in Indonesia

Quality and Proportion of Spermatozoa in the Percoll Density Gradient Centrifugation Sexing Method at Different Gradients using AndroMed® Diluent

The Quality and Proportion of Spermatozoa X and Y in Sexed Frozen Semen Separated with Percoll Density Gradient Centrifugation Method on Friesian Holstein Bull

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