Evaluation of Monolithic Packed 96‐Tips for Solid‐Phase Extraction of Local Anesthetics from Human Plasma for Quantitation by Liquid Chromatography Tandem Mass Spectrometry

Altun, Zeki; Hjelmström, Anette; Blomberg, Lars G.; Abdel‐Rehim, Mohamed

doi:10.1080/10826070701854030

Cited by 30 publications

(9 citation statements)

References 10 publications

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“…In tips developed in‐house, a packed 96‐tip assembly was used for the extraction of medication in human plasma 60, 200–204. All the previous articles are classified as methacrylate porous polymer.…”

Section: Design and Applications Of Offline Extraction Devicesmentioning

confidence: 99%

Monolith as a new sample preparation material: Recent devices and applications

Namera¹,

Nakamoto²,

Saito³

et al. 2011

J of Separation Science

107

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Monolith was first used as a material for chromatographic separation two decades ago and solid-phase extraction over 10 years, and since then, separation science has undergone a dramatic change owing to advancements in analytical technology. Recently, monolith has been modified to suit various devices for the extraction and enrichment of analytes in any matrices of environmental, food, and biological analyses. This approach has contributed to miniaturization and automation for sample preparation, and it can reduce the time and cost requirements of sample preparation. Recently, numerous applications have been demonstrated for online and inline preconcentration coupled with monolith, and many kinds of devices have been designed and developed for offline devices. In this review, these applications and devices are listed and discussed in reference to other fields.

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Section: Design and Applications Of Offline Extraction Devicesmentioning

confidence: 99%

Monolith as a new sample preparation material: Recent devices and applications

Namera¹,

Nakamoto²,

Saito³

et al. 2011

J of Separation Science

107

View full text Add to dashboard Cite

show abstract

“…Since the development of solid‐phase extraction (SPE), there has been a variety of modifications to simplify the technique (Abdel‐Rehim et al, ). Recently we introduced two microextraction techniques: microextraction by packed syringe (Abdel‐Rehim, ) and monolithic packed 96‐tips (Altun et al, , , , , ; Altun and Abdel‐Rehim, ). Microextraction by packed sorbent (MEPS) is the miniaturization of conventional solid‐phase extraction (SPE), with the packed bed (1–2 mg) incorporated directly in an injection syringe.…”

Section: Introductionmentioning

confidence: 99%

Screening and determination of drugs in human saliva utilizing microextraction by packed sorbent and liquid chromatography–tandem mass spectrometry

Abdel-Rehim

Abdel‐Rehim

2013

Biomedical Chromatography

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This study presents a new method for collecting and handling saliva samples using an automated analytical microsyringe and microextraction by packed syringe (MEPS). The screening and determination of lidocaine in human saliva samples utilizing MEPS and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were carried out. An exact volume of saliva could be collected. The MEPS C8 -cartridge could be used for 50 extractions before it was discarded. The extraction recovery was about 60%. The pharmacokinetic curve of lidocaine in saliva using MEPS-LC-MS/MS is reported.

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“…Recently we introduced a new sample preparation technique, a 96‐tips set packed with a plug of a monolithic adsorbent. Using such a set it is possible to handle a 96‐well plate in only 2 min (Altun et al ., , , ; Abdel‐Rehim et al ., ; Altun et al , ). Packed 96‐tips sample preparation is a clean, high‐throughput and automated method.…”

Section: Introductionmentioning

confidence: 99%

Monolithic packed 96‐tips set for high‐throughput sample preparation: determination of cyclophosphamide and busulfan in whole blood samples by monolithic packed 96‐tips and LC‐MS

Skoglund

Bassyouni

Abdel‐Rehim

2012

Biomedical Chromatography

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A monolithic methacrylate packed 96-tips device was used for the extraction of the busulfan and cyclophosphamide in whole blood samples. Using a packed 96-tips set, a 96-well plate could be handled in about 2 min. The key aspect of the monolithic phase is that monolithic material can offer both good extraction capacity and low-back-pressure properties. The validation of the methodology showed that the accuracy values of quality-control samples were between 99 and 113% for busulfan, and between 103 and 110% for cyclophosphamide. The inter-day precision ranged from 7.0 to 12% for busulfan and from 13 to 16% for cyclophosphamide. The standard calibration curves were obtained within the concentration range 5-2000 nm for busulfan and from 10 to 5000 nm for cyclophosphamide in blood samples. The coefficients of determination were ≥0.99.

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Evaluation of Monolithic Packed 96‐Tips for Solid‐Phase Extraction of Local Anesthetics from Human Plasma for Quantitation by Liquid Chromatography Tandem Mass Spectrometry

Cited by 30 publications

References 10 publications

Monolith as a new sample preparation material: Recent devices and applications

Monolith as a new sample preparation material: Recent devices and applications

Screening and determination of drugs in human saliva utilizing microextraction by packed sorbent and liquid chromatography–tandem mass spectrometry

Monolithic packed 96‐tips set for high‐throughput sample preparation: determination of cyclophosphamide and busulfan in whole blood samples by monolithic packed 96‐tips and LC‐MS

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