Screening and determination of drugs in human saliva utilizing microextraction by packed sorbent and liquid chromatography–tandem mass spectrometry

Abstract

This study presents a new method for collecting and handling saliva samples using an automated analytical microsyringe and microextraction by packed syringe (MEPS). The screening and determination of lidocaine in human saliva samples utilizing MEPS and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were carried out. An exact volume of saliva could be collected. The MEPS C8 -cartridge could be used for 50 extractions before it was discarded. The extraction recovery was about 60%. The pharmacokinetic curve of lidocaine in saliva using MEPS-LC-MS/MS is reported.

Dried saliva spot as a sampling technique for saliva samples

“…However, saliva sample collection and sample treatment have not been significantly developed. Recently, a new procedure for the collection and handling of saliva samples, using an automated analytical micro syringe (eVol) with microextraction by packed sorbent, for the screening and determination of lidocaine in human saliva samples was reported (Abdel‐Rehim and Abdel‐Rehim, ).…”

Microextraction Techniques in Illicit Drug Testing: Present and Future

“…DPX was also described in the 96-well plates format for high-throughput cleanup [14]. An additional improvement of MEPS for OF testing has been described in a recent article and consists of the introduction of a catheter to aspirate saliva directly from the mouth into the MEPS syringe [15].…”

Microextraction by Packed Sorbent (MEPS) and Solid-Phase Microextraction (SPME) as Sample Preparation Procedures for the Metabolomic Profiling of Urine

“…It is a miniaturization of the conventional solid-phase extraction (SPE)-packed bed devices from milliliter bed volumes to microliter volumes, which can be connected online to gas chromatography (GC) and/or liquid chromatography (LC) without any further modifications [33]. This technique has been successfully used to extract a wide range of analytes in different biological matrices, such as urine, plasma, saliva and blood [11,34,35]. …”

“…Sample extraction takes place in this packed bed, which can be coated to provide selective and suitable sampling conditions. The MEPS approach to sample preparation is suitable for reversed phases (extraction of hydrophobic analytes or polar organic analytes from aqueous matrices), normal phases (extraction of polar analytes from non-polar organic solvents) and mixed mode and ion exchange chemistries (extraction of charged analytes from aqueous or non-polar organic samples) [9,34,35]. There are several available MEPS sorbent materials (Figure 3), including reversed phase (C18, C8 and C2), normal phase (silica), restricted access material (RAM), HILIC (hydrophilic interaction liquid chromatography), carbon, polystyrene-divinylbenzene copolymer (PS-DVB), molecular imprinted polymers (MIPs), strong cation exchange (SCX) and mixed mode (C8/SCX) chemistries (reviewed in [9,35]).…”