Evaluation of Mesenchymal Stem Cells and Osteoblasts’ Adhesion and Proliferation in the Presence of HA-AL Biomaterials

Abstract

There is an increased interest in developing biocomposite implants with high biocompatibility in order to be used as grafts or prostheses in orthopedic surgery. The purpose of the study was to determine the biocompatibility of titanium implants coated with synthesized hydroxyapatite-alendronate composites. The implants were obtained using Matrix Assisted Pulsed Laser Evaporation technique (MAPLE). The hydroxyapatite-alendronate composites were synthesized using the wet precipitation method. Immunofluorescence microscopy showed that composites support mesenchymal stem cells (MSCs) adhesion. Bone cells as well as human MSCs adhere to hydroxyapatite (HA)-based thin films obtained by matrix assisted laser deposition onto titanium. Alendronate doping into the films increased the number of cell-biomaterial focal points as compared to HA only. Thus, the synthesis of hydroxyapatite-alendronate composite (HA-AL) may be considered a viable solution for including the bisphosphonate on the surface of metallic prosthetic components used in orthopedics.

In Vitro Characterization of Hydroxyapatite-Based Biomaterials, Using Mesenchymal Stem Cell Cultures From Human Bone Marrow

“…At passage 2, the cells were subjected to immunophenotyping by flow cytometry for validation of specific markers and then used in experiments or cryopreserved. For all experiments performed in the study, cells were seeded at a density of 5000 cells/cm 2 [12].…”

Process Optimization Using Quality by Design (Qbd) Approach of a Gentamicin Loaded Plga Biocomposite

“…Researchers found that the most effective treatment strategy is to use antibiotic loaded biocomposite based implants which contain the drug either attached to the surface or incorporated in bulk [13]. Synthetic polymers such as poly(-methylmethacrylate) or poly(D,Llactic-co-glycolic acid) [14] and natural polymers that include collagen, cellulose, chitosan [15] have been used either in association or not with hydroxyapatite [16,17] for the synthesis of such implants. Moreover, implants may include antibiotics such as ciprofloxacin, gentamycin, oxacillin, or vancomycin [18,19].…”

The Chemisorption-Release and Antibacterial Potential Studies of Ciprofloxacin From Hydroxyapatite-Based Implants

“…Local drug delivery systems, consisting of non-biodegradable or biodegradable and osteoactive materials, such as calcium orthophosphates, bone cement, have proved to be promising alternatives for the treatment of osteomyelitis [5]. These systems allow local delivery of the antibiotic in situ with bactericidal concentrations for long periods of time and without the toxicity associated with other means of administration [6].…”