Evaluation of mast cells in periapical cysts, dentigerous cysts, and keratocystic odontogenic tumors

Netto, Juliana De Noronha Santos; Pires, Fábio Ramôa; Fonseca, Elize Massard da; Silva, Licínio Esmeraldo da; Lourenço, Simone de Queiroz Chaves

doi:10.1111/j.1600-0714.2012.01126.x

Cited by 22 publications

(32 citation statements)

References 19 publications

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“…These studies showed that the frequency of mast cells was higher in areas near the implanted biomaterial, suggesting that the cells produced the inflammatory response induced by the studied materials. According to de Noronha Santos Netto et al 26 , a significantly greater number of mast cells was observed in lesions with inflammation based on an analysis of the frequency and distribution of mast cells in inflamed and non-inflamed cysts. These data indicate that mast cells participate in various acute and chronic inflammatory responses.…”

Section: Discussionmentioning

confidence: 99%

Effect of laser therapy on the inflammatory response induced by endodontic medications implanted into the subcutaneous tissue of rats

Matos

Soares

Júnior

et al. 2014

Rev. odontol. UNESP

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ResumoIntrodução: Medicamentos endodônticos apresentam componentes tóxicos que provocam algum grau de reação inflamatória. Objetivo: Este estudo avaliou o efeito da laserterapia na resposta inflamatória causada por medicações intracanais, em tecido subcutâneo de ratos, por meio da análise quantitativa de mastócitos. Material e método: Tubos de polietileno contendo as medicações foram implantados no dorso de 60 ratos, distribuídos em seis grupos: HS (pasta de hidróxido de cálcio P.A.); HL (pasta de hidróxido de cálcio P.A. e laserterapia); HPS (pasta de hidróxido de cálcio P.A. com paramonoclorofenol canforado); HPL (pasta de hidróxido de cálcio P.A. com paramonoclorofenol canforado e laserterapia); IS (iodofórmio e soro fisiológico) e IL (iodofórmio, soro fisiológico e laserterapia). Os animais foram eutanasiados oito e quinze dias após a cirurgia, as peças cirúrgicas foram removidas, processadas para inclusão em parafina e os cortes histológicos corados em Azul de Toluidina 0.2%, para quantificação dos mastócitos. A análise de variância (ANOVA) e o teste de Tukey post hoc foram aplicados para determinar diferenças significativas entre os grupos quanto ao número de mastócitos (p<0.05). Resultado: Nos grupos HL, HPL e IL houve uma diminuição de mastócitos em ambos os períodos experimentais em relação aos grupos HS, HPS e IS, porém não se observou diferença estatística significativa entre o grupo HPS e o HPL aos oito dias. Conclusão: A laserterapia foi eficaz em modular a intensidade da resposta inflamatória induzida pelos medicamentos endodônticos a partir da redução significativa na quantidade de mastócitos.Descritores: Endodontia; laserterapia; mastócitos; inflamação; subcutâneo. Abstract Introduction: Endodontic medications contain toxic components that cause varying degrees of inflammation.Objective: This study evaluated the effect of laser therapy on the inflammatory response induced by intracanal medications implanted into the subcutaneous tissue of rats using a quantitative analysis of mast cells. Material and method: Polyethylene tubes containing the medications were implanted in the dorsum of 60 rats divided into six groups, including HS (P.A. calcium hydroxide paste), HL (P.A. calcium hydroxide paste and laser therapy), HPS (P.A. calcium hydroxide paste with camphorated paramonochlorophenol), HPL (P.A. calcium hydroxide paste with camphorated paramonochlorophenol and laser therapy), IS (iodoform with saline) and IL (iodoform with saline and laser therapy). The animals were euthanized eight or fifteen days after surgery, and samples were removed and embedded in paraffin. Histological sections were stained with 0.2% toluidine blue for the quantification of mast cells. Analysis of variance (ANOVA) and Tukey's post-hoc test were applied to determine significant differences in the number of mast cells between groups (p<0.05). Result: There was a decrease in mast cells for the HL, HPL and IL groups when compared with the HS, HPS and IS groups at both time points. There was no statistically significant differe...

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Section: Discussionmentioning

confidence: 99%

Effect of laser therapy on the inflammatory response induced by endodontic medications implanted into the subcutaneous tissue of rats

Matos

Soares

Júnior

et al. 2014

Rev. odontol. UNESP

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“…1,2 Odontogenic cysts are derived from the epithelium of the dental apparatus and constitute more than 95% of the cysts of the jaws. The far most common odontogenic cysts are RCs, DCs, and OKCs.…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Assessment of Mast Cells and Small Blood Vessels in Dentigerous Cyst, Odontogenic Keratocyst, and Periapical Cyst

Kouhsoltani¹,

Khiavi²,

Jamali³

et al. 2015

Adv Pharm Bull

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“…Considering that mast cells are not easily recognized by hematoxylin and eosin, and may be confused with a variety of other cells and immature granulocytes 4 , the authors of the present study chose to assess these cells through toluidine blue staining. Since toluidine blue is a cationic dye, it exhibits the capacity to selectively integrate with the glycosaminoglycan-protein complex (highly ionized) 11 .…”

Section: Discussionmentioning

confidence: 99%

“…Santos Netto et al 4 analyzed mast cells using the toluidine blue technique and an immunohistochemical technique involving anti-tryptase antibody (clone G3) with 20 periapical cysts, 20 dentigerous cysts (14 inflamed and six non-inflamed) and 20 keratocystic odontogenic tumors. They noted that the mean number of mast cells found using the immunohistochemical technique was higher than when using histochemistry (P <0.0001).…”

Section: Discussionmentioning

confidence: 99%

“…Such pathological condition may destroy bone and may grow expansively as a consequence of the collapse of the extracellular matrix, leading to the accumulation of osmotic pressure in cystic fluid and/or perilesional bone resorption. The exact mechanism associated with this growth and expansion remains unclear, although it is known that several types of cells, including mast cells, do play a role in such phenomena 4 .…”

Section: Introductionmentioning

confidence: 99%

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Analysis of the presence and location of mast cells in periapical cysts and periapical granulomas

Nogueira

Farias

Silva

et al. 2016

RGO, Rev. Gaúch. Odontol.

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Objective The aim of the present study was to locate mast cells in chronic periapical lesions (granulomas and cysts) by using histochemical techniques and toluidine blue staining. Methods RESUMO ObjetivoLocalizar mastócitos em lesões periapicais (granulomas e cistos) através de técnicas histoquímicas e corante azul de toluidina. MétodosUma pesquisa quantitativa, descritiva, transversal e retrospectiva foi realizada. As amostras foram obtidas de documentos histopatológicos nos arquivos do laboratório de patologia cirúrgica da Universidade de Pernambuco entre Novembro de 2014 e Março de 2015. ResultadosDezesseis casos de granuloma e 21 casos de cistos periapicales foram selecionados. As lâminas coradas foram analisadas por dois examinadores em tempos diferentes, em um estudo duplo-cego. Mastócitos foram encontrados em 13 (61,9%) dos casos de cistos periapicales, localizados na cápsula da lesão. Nos granulomas periapicais, mastócitos foram encontrados em oito casos (50%), localizados no tecido de granulação. ConclusãoMastócitos foram detectados tanto em cistos quanto em granulomas periapicais, localizados na cápsula e no tecido de granulação respectivamente. Os mastócitos estavam mais presentes nos cistos do que nos granulomas periapicais.

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Evaluation of mast cells in periapical cysts, dentigerous cysts, and keratocystic odontogenic tumors

Cited by 22 publications

References 19 publications

Effect of laser therapy on the inflammatory response induced by endodontic medications implanted into the subcutaneous tissue of rats

Effect of laser therapy on the inflammatory response induced by endodontic medications implanted into the subcutaneous tissue of rats

Immunohistochemical Assessment of Mast Cells and Small Blood Vessels in Dentigerous Cyst, Odontogenic Keratocyst, and Periapical Cyst

Analysis of the presence and location of mast cells in periapical cysts and periapical granulomas

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