Immunohistochemical Assessment of Mast Cells and Small Blood Vessels in Dentigerous Cyst, Odontogenic Keratocyst, and Periapical Cyst

Kouhsoltani, Maryam; Khiavi, Monir Moradzadeh; Jamali, Golshan; Farnia, Samira

doi:10.15171/apb.2015.086

Cited by 4 publications

(7 citation statements)

References 19 publications

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“…However, there was a significant difference in MVD between RCs and DCs and between OKCs and DCs. These findings were in agreement with Kouhsoltani et al 40 who found that the highest mean numbers of microvessels was showed in RC. The higher MVD in RCs seen in the present study might be related to the presence of an exuberant inflammatory infiltrate in these lesions, because, according to Graziani et al 41 , Nonaka et al 42 and Tete et al 43 inflammatory cells can exert angiogenic activity in these cysts.…”

Section: Omnia Mohamedsupporting

confidence: 93%

“…It was found that inflamed OKCs and DCs showed higher mean numbers of microvessels compared to their noninflamed counterparts with statistically significant difference. This was in accordance with Kouhsoltani et al 40 who thought that the inflammatory reactions increase angiogenic activity in odontogenic cysts. They stated that inflammatory cells are generally considered to be related to angiogenesis in any lesions.…”

Section: Omnia Mohamedsupporting

confidence: 92%

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Angiogenesis and mast cells as predictive factors in behavior of Odontogenic cysts

2020

Mansoura Journal of Dentistry

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Purpose:The aim of this study was to verify the density of mast cells (MCs) and microvessels in odontogenic cysts. Furthermore, the correlation between MCs and microvessels was evaluated to assess the contribution of MCs to angiogenesis and growth of odontogenic cysts. This approach may be a basis for the development of future pharmaceuticals addressed to MCs performance to manage odontogenic cysts. Methods:45 cases of odontogenic cysts consisting of 15 radicular cysts (RCs), 15 dentigerous cysts (DCs) and 15 odontogenickeratocysts (OKCs) were included in this study. Five high power fields in connective tissue wall were counted for each sample. Moreover, a total mean of five fields was calculated to calculate MVD and MCD of the samples. Results: RC showed the highest mean numbers of microvessels (p<0.05) and OKC showed the highest mean number of mast cells. A positive correlation between the numbers of MCs and microvessels was observed. Conclusion: Increase in angiogenesis of OKC confirms that aggressive lesions require a larger number of blood vessels and highlights the concept of unique clinical behavior of this cyst. In OKCs and DCs cysts, the MVD is closely related to of the inflammatory infiltrate. Mast cells are imperative in the pathogenesis of odontogenic cysts. Mast cells have a role in promoting the growth of odontogenic cysts, particularly OKC. Further studies on the in vivo functions of MCs will make the concept more clear.

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Section: Omnia Mohamedsupporting

confidence: 93%

Section: Omnia Mohamedsupporting

confidence: 92%

Angiogenesis and mast cells as predictive factors in behavior of Odontogenic cysts

2020

Mansoura Journal of Dentistry

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“…These results corroborate those evidenced by other authors who demonstrated greater immunolabeling in RC compared to IDC and in IDC in relation to their non-inflammatory counterpart. 6,19 According to Netto et al, 19 the higher concentration of mast cells observed in inflammatory lesions could indicate their participation in the inflammatory response associated with odontogenic lesions. The higher MCD in RCs seems to be due to the active presence of a constant source of antigenic stimulation caused by bacterial toxins involving a greater variety of immunological cell types, including mast cells.…”

Section: Discussionmentioning

confidence: 98%

“…5 Differently, DCs are developmental odontogenic cysts associated with impacted teeth. 6 However, it has been suggested that periapical inflammation of non-vital deciduous teeth near the follicles of their respective unerupted permanent successors may trigger cystic formations known as inflammatory dentigerous cysts (IDC). 7 In flammation and the subpopulations of inflammatory cells are crucial for the development of the aforementioned lesions.…”

Section: Introductionmentioning

confidence: 99%

Involvement of tryptase-positive mast cells and angiogenesis in the growth of inflammatory odontogenic cysts

et al. 2021

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Inflammatory periapical lesions are characterized by infiltration of different immune cell types, the functions of which depend on an effective vascular network. This study aimed to evaluate the mast cells density (MCD) in inflamatory odontogenic cysts capsules concerning microvascular density (MVD), microvascular area (MVA), and microvascular perimeter (MVP), and correlate such findings with the type of lesion, intensity of the inflammatory infiltrate, and thickness of the epithelial lining. Twenty inflamatory dentigerous cysts (IDCs), twenty radicular cysts (RCs), and twenty residual radicular cysts (RRCs) were submitted to immunohistochemical analysis using anti-tryptase and anti-CD34 antibodies. RCs exhibited the highest MCD, MVD, MVA, and MVP indexes (p = < 0.001, p = 0.008, p = 0.003 and p = < 0.001, respectively), and lesions with inflammatory infiltrate grade III showed the highest MVD (p = 0.044). Considering epithelial thickness, a higher MVP index was identified in lesions with hyperplastic epithelium (p = 0.018). In IDCs, RCs, and RRCs, a strong positive correlation was observed between MVA and MVP (r = 0.950 and p = < 0.001; r = 0.914 and p = < 0.001; r = 0.713 and p = < 0.001, respectively). In IDCs, a moderate correlation was observed between MCD and both MVA and MVP (r = 0.660 and p = 0.002; r = 0.634 and p = 0.003, respectively). These results suggest that tryptase-positive mast cells might play an important role in the angiogenic activity of IDCs, while RCs had the highest indexes. Our findings also confirmed that the intensity of the inflammatory infiltrate and epithelial thickness influence angiogenesis.

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“…OKC is a common oral disease arising from the odontogenic epithelium, which has characteristics similar to those of malignant tumors such as aggressive growth and high recurrence ( 28 ). Tumor development always requires angiogenesis and the expression of numerous cytokines; OKC also has this characteristic ( 29 – 31 ). The aim of the present study was to elucidate the regulation of OKC, its mechanism of action and disease progression.…”

Section: Discussionmentioning

confidence: 99%

The TGF‑β1/COX‑2‑dependant pathway serves a key role in the generation of OKC‑induced M2‑polarized macrophage‑like cells and angiogenesis

et al. 2020

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An odontogenic keratocyst (OKC) is a common oral cyst arising from the odontogenic epithelium, which has the characteristics of a tumor. Previous studies have demonstrated that M2-polarized macrophages and angiogenesis have important roles in the progression of OKCs. As transforming growth factor (TGF)-β1 is important in growth and developmental processes, and early studies have indicated that TGF-β1 is upregulated in OKCs, the present study aimed to investigate the expression levels of TGF-β1 as a first step. Flow cytometric analysis suggested that TGF-β1 induced M2-polarization of macrophages in a dose-dependent manner. Expression levels of cyclooxygenase (COX)-1 and-2 were measured after treatment of M2 macrophages with TGF-β1 and OKC homogenate supernatant. COX-2 expression was influenced by TGF-β1 in a concentration-dependent manner and in OKC induction. In addition, inhibition of COX-2 resulted in the induction of M2-polarization of macrophages via TGF-β1 and OKC disruption. Because the extracellular matrix (ECM) is altered in individuals with chronic diseases, the present study analyzed the expression of matrix metalloproteinase (MMP)-9, which is able to degrade the ECM. The present study observed a decrease in MMP-9 activity following treatment with TGF-β1 and OKC homogenate supernatant. Additionally, the present study analyzed tube formation caused by OKC with or without a COX-2 inhibitor. The results of the present study suggested that angiogenesis increased following treatment with OKC homogenate supernatant but decreased after treatment with a COX-2 inhibitor. These findings indicated that the TGF-β1/COX-2 pathway may have an important role in the progression of OKC.

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Immunohistochemical Assessment of Mast Cells and Small Blood Vessels in Dentigerous Cyst, Odontogenic Keratocyst, and Periapical Cyst

Cited by 4 publications

References 19 publications

Angiogenesis and mast cells as predictive factors in behavior of Odontogenic cysts

Angiogenesis and mast cells as predictive factors in behavior of Odontogenic cysts

Involvement of tryptase-positive mast cells and angiogenesis in the growth of inflammatory odontogenic cysts

The TGF‑β1/COX‑2‑dependant pathway serves a key role in the generation of OKC‑induced M2‑polarized macrophage‑like cells and angiogenesis

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Immunohistochemical Assessment of Mast Cells and Small Blood Vessels in Dentigerous Cyst, Odontogenic Keratocyst, and Periapical Cyst

Cited by 4 publications

References 19 publications

Angiogenesis and mast cells as predictive factors in behavior of Odontogenic cysts

Angiogenesis and mast cells as predictive factors in behavior of Odontogenic cysts

Involvement of tryptase-positive mast cells and angiogenesis in the growth of inflammatory odontogenic cysts

The TGF‑&beta;1/COX‑2‑dependant pathway serves a key role in the generation of OKC‑induced M2‑polarized macrophage‑like cells and angiogenesis

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The TGF‑β1/COX‑2‑dependant pathway serves a key role in the generation of OKC‑induced M2‑polarized macrophage‑like cells and angiogenesis