Evaluation of hydrolysed rubber seed meal as a dietary protein source for Nile tilapiaOreochromis niloticusL.

Abstract

This study evaluated the use of hydrolysed rubber seed meal as a locally grown alternative feed ingredient to reduce the use of fishmeal within formulated feeds for tilapia. Five experimental diets were prepared by formulating different inclusion levels of hydrolysed rubber seed meal (0%, 25%, 50%, 75% and 100% of plant‐based protein contribution). Dried and defatted rubber seed meal was hydrolysed by adding rumen digesta liquid to the ingredient. For digestibility measurement, chromic oxide was added to each diet as an inert marker. The digestibility trial was conducted over a 14‐day period by collecting faecal matter from the rearing tanks. In addition, a growth trial was conducted over a period of 50 days using four replicates per dietary treatment. In both experiments, all fish were fed three times daily to apparent satiation. At the end of growth trial, the inclusion level of hydrolysed rubber seed meal up to 50% of plant‐based protein contribution did not give any significant difference compared to the control diet in terms of feed efficiency, specific growth rate and protein retention parameters. In the digestibility study, fish fed diets containing hydrolysed rubber seed meal up to a 50% of plant‐based protein inclusion level did not give any significant difference compared to the control diet in terms of protein and dry matter apparent digestibility. Based on the result of this growth and digestibility study, up to a 50% of plant‐based protein contribution of hydrolysed rubber seed meal can be used within formulated tilapia feeds without any adverse effect.

Influence of replacing fish meal with enzymatic hydrolysates of defatted silkworm pupa (Bombyx moriL.) on growth performance, body composition and non-specific immunity of juvenile mirror carp (Cyprinus carpio var. specularis)

“…Enzyme hydrolysis of protein promotes the improvement or modification of the physicochemical, functional and sensory properties of the native protein without affecting its nutritional value (Chalamaiah, Rao, Rao, & Jyothirmayi, 2010). Extensive studies have indicated that protein hydrolysates could be used as a high-quality protein source and have been widely used in aquatic feed including crucian carp (Dan, Wen, Shao, & Xu, 2010;Liu, Zhan, & Wang, 2007), carp (Feng, Yang, Zhou, Jiang, & Liu, 2008), shrimp (Leng, Wang, Zhou, & Liu, 2006), Juvenile Red Sea Bream (Bui, Khosravi, Fournier, Herault, & Lee, 2014) and tilapia (Suprayudi et al, 2017). In this study, no significant differences (p > .05) in most investigated parameters concerning fish growth performance including FBW, WG, SGR, FCR and PER were detected among EHDSP0, EHDSP25 and EHDSP50 groups.…”

“…Finally, it can promote the growth of fish by enhancing the ability of absorbing nutrients. Suprayudi et al (2017) found that only up to 50% hydrolysed rubber seed meal as a dietary protein source for tilapia can be used without any adverse effect. Furthermore, several studies pointed out that dietary inclusion of enzymatic protein hydrolysates at higher level can cause negative effects (Espe, Sveier, Hogoy, & Lied, 1999;Hevrøy et al, 2005).…”

Evaluation of rubber seed oil as lipid source in red tilapia ( Oreochromis sp.) diet

“…In tilapia fed with these feed, there were no significant differences in protein and lipid retention compared with controls (CTRL). In accordance with this, Suprayudi et al () and Fawole et al () had demonstrated that HCN reduction in feed ingredients will maintain protein and lipid retention rates in tilapia and rohu. This is due to the lower antinutritional factors that block the metabolic process and retain nutrients.…”

“…This is seen from no significant differences in the case of specific growth rates of tilapia between treatments that include heated RSO feed (CPO: PRSO and PRSO). Previous research using the rubber seed‐based feed which has reduced the HCN levels has a similar specific growth rate and not significantly different from the control feed relatively (Deng et al, ; Fawole et al, ; Suprayudi et al, , ). The condition confirmed that these previous study results are in accordance with this current study.…”

“…Lipid and lipoprotein serum levels of red tilapia that were fed by heated RSO feed (CPO:PRSO and PRSO) were not significantly different from controls (CTRL). Previously, studies that were demonstrated by Suprayudi et al () showed that the use of rubber‐based feed with further processing showed an increase in cholesterol level. Several studies in terrestrial animals have shown that an increase in cholesterol can be triggered by high cholesterol in feed ingredients (Kaushik et al, ; Lim & Lee, ; Refstie, Svihus, Shearer, & Storebakken, ) However, there is still not clear do the high cholesterol, HDL and LDL have the similar risk in cardiovascular effect in animals as the same as in humans.…”