This study evaluated the use of hydrolysed rubber seed meal as a locally grown alternative feed ingredient to reduce the use of fishmeal within formulated feeds for tilapia. Five experimental diets were prepared by formulating different inclusion levels of hydrolysed rubber seed meal (0%, 25%, 50%, 75% and 100% of plant‐based protein contribution). Dried and defatted rubber seed meal was hydrolysed by adding rumen digesta liquid to the ingredient. For digestibility measurement, chromic oxide was added to each diet as an inert marker. The digestibility trial was conducted over a 14‐day period by collecting faecal matter from the rearing tanks. In addition, a growth trial was conducted over a period of 50 days using four replicates per dietary treatment. In both experiments, all fish were fed three times daily to apparent satiation. At the end of growth trial, the inclusion level of hydrolysed rubber seed meal up to 50% of plant‐based protein contribution did not give any significant difference compared to the control diet in terms of feed efficiency, specific growth rate and protein retention parameters. In the digestibility study, fish fed diets containing hydrolysed rubber seed meal up to a 50% of plant‐based protein inclusion level did not give any significant difference compared to the control diet in terms of protein and dry matter apparent digestibility. Based on the result of this growth and digestibility study, up to a 50% of plant‐based protein contribution of hydrolysed rubber seed meal can be used within formulated tilapia feeds without any adverse effect.
Produksi udang vannamei merupakan kegiatan yang menjadi primadona dalam bidang budidaya perikanan yang berbasis in land aquaculture. Kabupaten Lombok Utara mempunyai luas wilayah daratan 809.53 Km2 yang terdiri dari wilayah khusus (hutan lindung, kawasan margasatwa, dan lain-lain) seluas 361,86 Km2 (44,30%) dan sisanya daratan rata untuk lahan pertanian seluas 447,67 Km255,30%. Luas wilayah perairan Lombok Utara adalah 594,71 Km2 dengan panjang pantai 127 Km. Ditinjau dari luas daratan yang cukup luas sehingga diharapkan mampu menyumbangkan kebutuhan produksi akan udang vannamei yang selama ini hanya mengandalkan pemeliharaan di pesisir pantai. Pengabdian kepada masyarakat ini bertujuan memberi pemahaman dan meningkatkan kemampuan masyarakat yang baik dalam mengelola budidaya udang vannamei terutama menggunakan ruang atau lahan yang terbatas dan jauh dari pesisir pantai dan mengoptimalkan sumber air tawar yang ada. Metode yang digunakan dalam pengabdian ini adalah Focus Group Discusion (FGD) dengan memberikan informasi atau wawasan kepada pembudidaya terkait pengembangan teknologi budidaya udang salinitas rendah di kolam bundar dengan metode Bioflok serta pendampingan dan praktik. Penyuluhan mengenai pengembangan teknologi budidaya udang salinitas rendah di kolam bundar dengan sistem bioflok juga membantu pembudidaya dalam membudidayakan udang di air salinitas rendah menggunakan teknologi modern yaitu sistem bioflok yang dapat memperbaiki dan meningkatkan mutu kuantitas produksi budidaya mitra usaha Muda Mandiri di Desa Tanjung Kecamatan Tanjung, Lombok Utara, Nusa Tenggara Barat. Penyuluhan mengenai pengembangan teknologi budidaya udang salinitas rendah di kolam bundar dengan sistem bioflok juga dapat membantu pembudidaya memperbaiki dan meningkatkan mutu kualitas produk budidaya mitra usaha Muda Mandiri di Desa Tanjung Kecamatan Tanjung, Lombok Utara, Nusa Tenggara Barat.
A major drawback associated with an expression of a high-level Plasmodium Lactate Dehydrogenase (pLDH) using Escherichia coli is the low solubility due to the formation of an inclusion body (IB). This study aimed to develop a suitable protocol for enhancing the solubility of pLDH expressed in E. coli. Firstly, a pLDH-encoding gene was amplified from the blood sample of malaria-infected patients and ligated into pBlueScript II KS+ for sequencing. Afterward, the pLDH gene was digested and cloned into pColdTF for expression. The recombinant plasmid was transformed into the E. coli BL21 (DE3) RIPL Codon Plus Strain. Then, the bacterial host was initially cultured at 37°C until reaching optical density (OD) at 600 nm: 0.5. Thereafter, the growth temperature was lowered to 15°C, followed by the addition of 0.1 mM IPTG into the culture medium for inducing pLDH expression. Thereafter, the bacterial hosts were cultured in a cold-stirred bioreactor (15°C). The result showed that a combination of the low culture conditions (15°C) and a low amount of IPTG increased the solubility of pLDH. This result suggests that this protocol can be a convenient method for generating high-quality recombinant protein using the E. coli system.
Two advanced lines: BR8693-8-4-2-1 and BR8693-17-6-2-2, along with BRRI dhan49 (S. Ck) and BRRI dhan33 (R. Ck) as checks were tested at farmers’ field in eight locations such as West Byde (BRRI Gazipur), Khulna (Dumuria), Habiganj (Sadar), Chapainawabganj (Gomostapur), Rangpur (Sadar), Feni (Dagonbhuyan), Chattagram (Hathazari) and Barishal (Sadar) during the season of Transplanting Aman 2018. The trial was replicated three times in each location. The unit plot size was 17.94 m2 (4.6m x 3.9m). Seeding emergence for eight locations varied from 11-17 July 2018. Seedling age varied from 25-30 days among the locations. Seedlings were transplanted at 20 cm x 15 cm spacing. NPKS and Zn fertilizers were applied at the rate of 0, 15, 50, 12 and 3.6 kg ha-1, respectively. All fertilizers except urea were applied as basal whereas, urea was applied in 3 equal splits at 10, 25 and 40 days after transplanting (DAT) Other standard management practices were followed as and when necessary. No insecticide was used because the two advanced lines were insect resistant and diseases were not controlled (to identify susceptibility and tolerance level of lines). Date of seeding, transplanting, flowering and maturity, lodging tolerance, pest and disease incidence, phenotypic acceptance at vegetative and ripening stage, yield and yield components were recorded. Among the two advanced lines, BR8693-8-4-2-1 line was statistically significant from BR8693-17-6-2-2, BRRI Dhan49 and BRRI Dhan33 respectively and the disease reaction, farmers’ opinion, and special character of insect resistant, BR8693-8-4-2-1 was found to be superior for further variety trial. The main purpose of the experiment is to recommended insect resistant rice genotype for proposed variety trail. For yield estimation, 9 m2 sample area from each plot was harvested at maturity and grain yields were adjusted to 14% moisture content.
Pakan merupakan salah satu faktor yang menentukan keberhasilan budidaya ternak unggas dan udang. Permasalahan utama yang dihadapi oleh budidaya kedua komiditas tersebut adalah tingginya biaya pakan. Solusi yang ditawarkan untuk mengatasi permasalahan tersebut adalah optimalisasi penggunaan bahan lokal yang didukung oleh penggunaan sinbiotik. Sinbiotik merupakan kombinasi antara probiotik dan prebiotik yang dapat memberikan manfaat bagi peningkatan kecernaan pakan serta keseimbangan mikroorganisme saluran pencernaan ternak unggas dan udang untuk kesehatan. Tujuan dari kegiatan pengabdian ini adalah untuk meningkatkan pemahaman dan keterampilan peternak unggas dan udang dalam memproduksi sinbiotik sebagai campuran pakan berbahan baku lokal. Kegiatan pengabdian ini dilaksanakan di Desa Pijot Kecamatan Keruak Kabupaten Lombok Timur Provinsi Nusa Tenggara Barat. Metode yang digunakan pada kegiatan pengabdian ini adalah pelatihan pembuatan sinbiotik dan uji palatabilitas sinbiotik pada ternak unggas dan udang. Pelatihan pembuatan probiotik menyangkut penentuan jumlahdan jenis bahan baku sinbiotik, pencampuran probiotik dan prebiotik, pembuatan pellet (pelleting) dan proses pengeringan. Untuk mengetahui tingkat palatabilitas pada unggas dan udang, sinbiotik yang sudah dihasilkan diberikan ke ternak unggas dan udang. Penggunaan sinbiotik pada campuran pakan ternak unggas dan udang dapat mengurangi biaya pakan sehingga peternak mendapatkan keuntungan yang lebih tinggi. Kesimpulan dari kegiatan pengabdian ini adalah peternak dapat menerima inovasi dengan baik, mampu mempersiapkan bahan baku dan melaksanakan proses pembuatan sinbiotik.
Koi fish (Cyprinus carpio) is one of the potential freshwater ornamental fish commodities that can be cultivated. This freshwater ornamental fish has several advantages, including easy maintenance, ability to survive in low oxygen water, and ability to produce high-quality eggs. The purpose of this research is to assess the feasibility of the breeding activities conducted at BBI Batu Kumbung through economic analysis. The research method used in this study is "Descriptive Comparative Method". Based on the research findings the economic analysis conducted includes assumptions, Total Costs Rp. 39.304.000, Sales Revenue Per Cycle Rp. 64.200.000, Income Per Cycle Rp. 24.896.000, R/C Ratio 1.63, Break-Even Point Price Rp. 1.224 per fish, Break-Even Point Production 19.652 and Payback Period 6.3 months. Based on the business analysis, the production of koi fish seeds at BBI Batu Kumbung can be considered feasible and has the potential for development, as it can provide good profits with a good Return on Investment (ROI).
In Bangladesh, the bitter gourd, which is a vegetable, is a wonderful source of magnesium, folate, zinc, phosphorus, and vitamins B1, B2, and B3. It also contains a high level of dietary fiber. From December 2020 to April 2021, an experiment was carried out in the field laboratory of Bangladesh Agricultural University, Mymensingh, to determine how the polyhouse and the local environment affected the growth and yield of bitter gourd during the off-season. It was a factorial experiment using RCBD (Randomized Completely Block Design), in which Factor A represented the impact of a polyhouse and Factor B represented the influence of the environment on the development and yield of bitter gourd during the off-season. Results showed that photochemically active radiation decreased within the polyhouse compared to the open area outside. In terms of soil temperature, soil moisture, and air temperature, a reverse trend was seen. In comparison to an open field during the winter, the polyhouse had higher air, soil, and soil moisture temperatures. Compared to plants cultivated in open fields, bitter gourd plants planted in poly houses had longer stems, more branches, blooms, and higher reproductive efficiency, which led to more fruit setting plants-1 and a higher fruit yield. Additionally, the fruit size in the polyhouse was greater than it was in the open field situation. In comparison to open fields, the fruit output of bitter gourd was approximately 2.5 times higher in polyhouses, which will be helpful to meet up the nutritional demand in the off-season.
Field experiment was carried out in Moddhochar Jabbar of Noakhali, Bangladesh during Boro season (December-April) 2018 to investigate the ameliorative effect of application of gypsum and transplanting method on the yield of BRRI dhan28 (V1) and BINAdhan-10 (V2)under salinity stress. The experiment comprised 3 factors; Transplanting method, Variety and Gypsum. This variety was evaluated under two transplanting methods i.e., Furrow transplanting (T1) and Conventional transplanting (T2) and four levels of gypsum with control G0: control, G1: 50 kg ha-1,G2: 100 kg ha-1 and G3: 150 kg ha-1 .The experiment was laid out in a Latin Square Design (LSD) with three replications. Salinity level had significant negative effect on plant characters and yield of rice. Gypsum (@ 150 kg/ha) exerted positive significant effect on most of the plant parameters except number of non-effective tillers. Furrow transplanting method (T1) had positive significant effect on most of the plant parameters except panicle length and harvest index. Binadhan-10 shows better performance than BRRI dhan28 in case of yield and all yield contributing characters in the prevailing condition. The highest grain yield (6.63 t ha-1) found from T1V2G3 (combination of furrow transplanting method, Binadhan-10 and gypsum @ 150 kg/ha) and lowest (4.26 t ha-1) inT2V1G0 (combination of conventional transplanting method, BRRI dhan28 and gypsum @ 0 kg ha-1). It was calculated that the straw yield highest (8.14 t ha-1) in T1V2G2 (combination of furrow transplanting method, Binadhan-10 and gypsum @ 100 kg/ha) and lowest (5.61 t ha-1) in T2V1G0 (combination of conventional transplanting method, BRRI dhan28 and gypsum @ 0 kg/ha).
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