Evaluation of antioxidant, in vitro cytotoxicity of micropropagated and naturally grown plants of Leptadenia reticulata (Retz.) Wight & Arn.-an endangered medicinal plant

Abstract

The strong cytotoxicity of ethyl acetate extract revealed anti carcinogenic potential of the plant which supports its traditional use as medicine. The present investigation is new to literature till date and will provide better scientific basis for future pharmacological, in vivo studies and novel source of pure bioactive compounds having anti cancer properties in this plant.

<em>Leptadenia reticulata</em> (Retz.) Wight (Jivanti): Botanical, Agronomical, Phytochemical, Pharmacological, and Biotechnological Aspects

“…Similarly, the DPPH free radical scavenging activity study showed the highest antioxidant potential in the ethyl acetate extract of L. reticulata with IC50 value of 267.13 μg/mL followed by the methanolic extract of L. reticulata with IC50 value of 510.15 μg/mL. Similarly, hydrogen peroxide scavenging and FeCl3 reducing method also recorded the highest antioxidant potential in ethyl acetate extract of L. reticulata with IC50 value of 234.1 μg/mL and 406.4 μg/mL, respectively [69]. In another study, antioxidant enzyme activity of in vitro regenerated and field-grown plants of L. reticulata were evaluated [72].…”

“…The ethyl acetate fraction and methanol fraction of L. reticulata were shown to exhibit a dose-dependent lipid peroxidation inhibition activity. The highest inhibitory activity (71.2%) was observed in ethyl acetate fraction followed by methanol fraction with 58.18% [69].…”

“…They observed that the extract of L. reticulata had a significant inhibitory effect on the proliferationof tumor cell. In vitro study conducted by Mohanty et al, [69] showed that the ethyl acetate extract of naturally grown L. reticulata was effective in inhibiting MCF-7, HT-29, and L6 cells with IC50 values of 21 μg/mL, 26 μg/mL, and 22 μg/mL, respectively. The ethyl acetate extract of micropropagated L. reticulata exhibited cytotoxicity against MCF-7, HT-29, and L6 cells with IC50 values of 20 μg/mL, 30 μg/mL, and 18 μg/mL, respectively.…”

In vivo hypoglycemic, antinociceptive and in vitro antioxidant activities of methanolic bark extract of Crataeva nurvala

“…Phytoconstitutes characterized in the current experiment are subject to be beneficial in pharmaceutical sciences [31]. The knowledge of this initial investigation can be anticipated as an interpretation in the exploration of a novel and financially valued drug molecules [32,33].…”

<i>In vitro</i> Cytotoxic and Antioxidant Activity of Leaf Extracts of Mangrove Plant, <i>Phoenix paludosa</i> Roxb

“…Different plant extracts exhibit different cytotoxic properties to different cancer cell lines which may be due to the presence of different cytotoxic phytochemicals and their different mode of action to different cancer cell lines [13]. Despite available reports on anti-oxidant, analgesic, antitumor, as a growth effector and anti-diarrheal properties [14] of this plant, this is the first comparative study of this plant on cytotoxicities in four different cancer cell lines and two normal cell lines.…”