&lt;i&gt;In vitro&lt;/i&gt; Cytotoxic and Antioxidant Activity of Leaf Extracts of Mangrove Plant, &lt;i&gt;Phoenix paludosa&lt;/i&gt; Roxb

Samarakoon, Sameera Ranganath; Shanmuganathan, Chanthirika; Tennekoon, Kamani Hemamala; Piyathilaka, Poorna; Thabrew, Ira

doi:10.4314/tjpr.v15i1.18

Cited by 17 publications

(7 citation statements)

References 14 publications

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“…Flavonoid content was also high in ethanolic leaf extract of C. rotang (458.85 mg/g) when compared with methanolic leaf extract of C. rotang (2.68 mg/g) [18]. The flavonoid content in methanolic extract of leaves of Acorus calamus was 22 mg/g, whereas in 70% ethanolic extract of A. calamus it was reported to be 1.48 mg/g [19], and in methanolic extract of leaf of Phoenix paludosa was 9 mg/g [20]. The total phenol and flavonoid contents in ethanolic leaf extract of C. rotang (49.04 mg/g and 458.85 mg/g) were found to be more than the total phenol and flavonoid contents in methanolic leaf extract of Colocasia esculenta (27 mg/g and 11 mg/g), respectively [21].…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Studies and High-Performance Thin-Layer Chromatography Analysis of Calamus Rotang Linn Leaf Extracts

Kpj

2018

Asian J Pharm Clin Res

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Objective: The present study was aimed at phytochemical screening, quantification, and high-performance thin-layer chromatography (HPTLC) analysis of hexane, chloroform and ethanol leaf extracts of Calamus rotang.Methods: Leaf extracts were prepared according to the polarity of the solvents, i.e., hexane, chloroform, and ethanol. Preliminary phytochemical screening involved the qualitative methods to detect the presence of alkaloids, phenols, flavonoids, saponins, steroids, etc. Quantitative estimation of alkaloids using boldine as standard, phenols using gallic acid as standard, and flavonoids using quercetin as standard were done. HPTLC analysis was done with all three extracts along with quercetin and rutin standards using mobile phase for flavonoids, i.e., 90:10 ratio of chloroform and methanol solvents.Results: Phytochemical screening showed the presence of phenols, flavonoids, alkaloids, etc. Hence, quantification was done for these phytochemicals. Alkaloids were present significantly more in hexane leaf extract, i.e., 2.54±0.216mg boldine equivalents/g. Phenols were present significantly more in ethanolic leaf extract, i.e., 49.04±0.364 mg gallic acid equivalents)/g. Flavonoids were present in significant amount in ethanolic leaf extract, i.e., 458.85±5.74 mg quercetin equivalents/g. HPTLC analysis of hexane, chloroform, and ethanolic extracts showed the presence of flavonoids such as quercetin, rutin, and some unknown flavonoid compounds.Conclusion: Ethanolic leaf extract showed a high amount of phenols and flavonoids. Hence, the extract can be further exploited further for in vitro and in vivo research work.

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Section: Discussionmentioning

confidence: 99%

Phytochemical Studies and High-Performance Thin-Layer Chromatography Analysis of Calamus Rotang Linn Leaf Extracts

Kpj

2018

Asian J Pharm Clin Res

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“…Were determined in the in-vitro cytotoxic activity of the methanol crude extract (MCE) and its active fractions against MCF-7 cell lines were determined using sulphorhodamine assay (SRB assay) as previously described by Samarakoon et al [10];however, a slight modification was done in the procedure. Briefly, cells were trypsinised and inoculated (5x10 3 cells/well) into 96-multiwell plates then incubated for 24 hours.…”

Section: Anti-proliferative Effect Of Methanol Extract Of Acacia Seyal Gummentioning

confidence: 99%

Active Fractions of Methanol Crude Obtained from Acacia Seyal Gum and their Antiproliferative Effects against Human Breast Cancer Cell Lines

Elnour¹,

Mirghani²,

Kabbashi³

et al. 2020

GJSFR

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Background: This study is on Acacia seyal gum, which is an exudate from Talha (Acacia seyal) tree. It provides a rich source of prebiotic that is used traditionally in folk medicine. Aims: The anti-proliferative effect (APE) of Acacia seyal gum (ASG) and Prebio-T-commercial (PTC) samples on human breast cancer (MCF-7) cell lines, and their antioxidant activities (AA) were investigated. Methods: The methanol crude extracts of both Acacia seyal gum and Prebio-T- commercial were fractioned into acetone and methanol, respectively. The anti-proliferative effect on human breast cancer cell lines for each fraction was examined using sulphorhodamine assay (SRB assay). Methanol crude extracts and their active compositions were analysed carefully using Gas chromatography-mass spectrometry technique.

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“…to ABTS is measured in the ABTS assay (Alam et al, ). DPPH and ABTS assays have been used in our recent studies to determine antioxidant effects of several plant extracts (Ediriweera, Tennekoon, Samarakoon, et al, ; Samarakoon et al, ). The reduction of a ferric complex to ferrous ions by potential antioxidants is the main principle of the ferric reducing‐antioxidant power assay (Alam et al, ).…”

Section: Assays For Oxidative Stressmentioning

confidence: 99%

In vitro assays and techniques utilized in anticancer drug discovery

Ediriweera

Tennekoon

Samarakoon

2018

J of Applied Toxicology

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Development of a cancer is a multistep process and six major hallmarks of cancer that are known to control malignant transformation have been described. Anticancer drug development is a tedious process, requiring a number of in vitro, in vivo and clinical studies. In vitro assays provide an initial platform for cancer drug discovery approaches. A wide range of in vitro assays/techniques have been developed to evaluate each hallmark feature of cancer and selection of a particular in vitro assay or technique mainly depends on the specific research question (s) to be examined. In the present review, we have described some commonly utilized in vitro assays and techniques used to examine cell viability/proliferation, apoptosis, cellular senescence, invasion and migration, oxidative stress and antioxidant effects, gene and protein expression, angiogenesis and genomic alterations in cancer drug discovery. Additionally, uses of modern techniques such as high throughput screening, high content screening and reporter gene assays in cancer drug discovery have also been described.

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<i>In vitro</i> Cytotoxic and Antioxidant Activity of Leaf Extracts of Mangrove Plant, <i>Phoenix paludosa</i> Roxb

Cited by 17 publications

References 14 publications

Phytochemical Studies and High-Performance Thin-Layer Chromatography Analysis of Calamus Rotang Linn Leaf Extracts

Phytochemical Studies and High-Performance Thin-Layer Chromatography Analysis of Calamus Rotang Linn Leaf Extracts

Active Fractions of Methanol Crude Obtained from Acacia Seyal Gum and their Antiproliferative Effects against Human Breast Cancer Cell Lines

In vitro assays and techniques utilized in anticancer drug discovery

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