Enzyme Immobilization on Porous Silicon Surfaces

Létant, Sonia E.; Hart, Bradley R.; Kane, Staci R.; Hadi, Masood Z.; Shields, Sharon J.; Reynolds, John G.

doi:10.1002/adma.200306173

Cited by 114 publications

(94 citation statements)

References 25 publications

(25 reference statements)

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“…During the experiment the following were used: acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7, acetylcholinesterase from human erythrocytes), acetylthiocholine iodide, 5,5 -dithio-bis(2-nitrobenzoic acid), neostigmine methyl sulfate and MgCl 2 , purchased from Sigma-Aldrich, NaCl (Daejung Chemical and Metals Co., Ltd), ethanol, water (Samchun Chemicals), HF (48 %, w/w; Merck) and boron-doped p-type silicon wafers with a resistivity of [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] cm and thickness 500-550 μm (obtained from Cree Co.). The photoluminescence spectra and relative photoluminescence intensities were measured on an FS-2 fluorescence spectrometer (Scinco) and LabRam HR-800 spectrometer (Horiba Jobin Yvon) with a He/Cd laser source (325 nm).…”

Section: Materials and Instrumentationmentioning

confidence: 99%

“…The silicon wafer (boron-doped p-type silicon wafers with resistivity [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] cm and thickness 500-550 μm) was cut into chips sized 1×1 cm 2 and degreased with an ultrasonic bath of acetone for 5 min, and then rinsed in deionized water. After drying with nitrogen gas, silver paste was simply sputtered on to the back of the wafer to provide a back ohmic contact for anodization.…”

Section: Preparation Of Porous Silicon Surfacementioning

confidence: 99%

“…The unique features of porous silicon make this material a frontline candidate for enzyme immobilization [3][4][5][6][7][8][9], drug delivery [10,11], energyharvesting devices [12][13][14][15][16][17] and biosensing [18,19], due to its large internal surface area, prodigious pore volume, biodegradability, and tunable pore geometry via variation of both porosity and crystallite size [20][21][22][23][24]. The biodegradation of porous silicon results in the formation of orthosilicic acid, which can easily be absorbed from the gastrointestinal tract and excreted from the body [25].…”

Section: Introductionmentioning

confidence: 99%

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Acetylcholinesterase immobilization and characterization, and comparison of the activity of the porous silicon-immobilized enzyme with its free counterpart

et al. 2016

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SynopsisA successful prescription is presented for acetylcholinesterase physically adsorbed on to a mesoporous silicon surface, with a promising hydrolytic response towards acetylthiocholine iodide. The catalytic behaviour of the immobilized enzyme was assessed by spectrophotometric bioassay using neostigmine methyl sulfate as a standard acetycholinesterase inhibitor. The surface modification was studied through field emission SEM, Fourier transform IR spectroscopy, energy-dispersive X-ray spectroscopy, cathode luminescence and X-ray photoelectron spectroscopy analysis, photoluminescence measurement and spectrophotometric bioassay. The porous silicon-immobilized enzyme not only yielded greater enzyme stability, but also significantly improved the native photoluminescence at room temperature of the bare porous silicon architecture. The results indicated the promising catalytic behaviour of immobilized enzyme compared with that of its free counterpart, with a greater stability, and that it aided reusability and easy separation from the reaction mixture. The porous silicon-immobilized enzyme was found to retain 50 % of its activity, promising thermal stability up to 90 • C, reusability for up to three cycles, pH stability over a broad pH of 4-9 and a shelf-life of 44 days, with an optimal hydrolytic response towards acetylthiocholine iodide at variable drug concentrations. On the basis of these findings, it was believed that the porous silicon-immobilized enzyme could be exploited as a reusable biocatalyst and for screening of acetylcholinesterase inhibitors from crude plant extracts and synthesized organic compounds. Moreover, the immobilized enzyme could offer a great deal as a viable biocatalyst in bioprocessing for the chemical and pharmaceutical industries, and bioremediation to enhance productivity and robustness.

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Section: Materials and Instrumentationmentioning

confidence: 99%

Section: Preparation Of Porous Silicon Surfacementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Acetylcholinesterase immobilization and characterization, and comparison of the activity of the porous silicon-immobilized enzyme with its free counterpart

et al. 2016

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“…Silicon-based particles have been long approved for medical use, 20 by employing SiO 2 matrices as delivery systems [21][22][23][24][25][26][27] for protein, enzymes, drugs, and genes. CeO 2 (ceria) NPs on the other hand were recently studied for their pharmacological potential 28 as drug delivery carriers by exploiting their multienzyme antioxidant mimetic properties.…”

Section: Introductionmentioning

confidence: 99%

Assessing the axonal translocation of CeO2 and SiO2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study

Kastrinaki

Samsouris

Kosmidis

et al. 2015

IJN

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The axonal translocation of two commonly used nanoparticles in medicine, namely CeO 2 and SiO 2 , is investigated. The study was conducted on frog sciatic nerve fibers in an ex vivo preparation. Nanoparticles were applied at the proximal end of the excised nerve. A nerve stimulation protocol was followed for over 35 hours. Nerve vitality curve comparison between control and exposed nerves showed that CeO 2 has no neurotoxic effect at the concentrations tested. After exposure, specimens were fixed and then screen scanned every 1 mm along their length for nanoparticle presence by means of Fourier transform infrared microscopy. We demonstrated that both nanoparticles translocate within the nerve by formation of narrow bands in the Fourier transform infrared spectrum. For the CeO 2 , we also demonstrated that the translocation depends on both axonal integrity and electrical activity. The speed of translocation for the two species was estimated in the range of 0.45–0.58 mm/h, close to slow axonal transportation rate. Transmission electron microscopy provided direct evidence for the presence of SiO 2 in the treated nerves.

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“…[1][2][3][4] It is of great importance to improve the biocompatibility of the silicon microdevices and to develop silicon surfaces with controllable cell adhesion. Immobilization of biomolecules on silicon substrates can be achieved via many methods such as adsorption, entrapment, covalent binding, etc.…”

Section: Introductionmentioning

confidence: 99%

Covalent Immobilization of Glucose Oxidase on Well-Defined Polymer-Silicon Wafer Hybrids via Surface-Initiated Raft-Mediated Process

Xia

Liu

et al. 2013

J. Chil. Chem. Soc.

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A surface modification technique was developed for the functionalization of silicon surface with glucose oxidase (GOD). The silicon surface was first graft copolymerized with glycidyl methacrylate (GMA) via surface-initiated reversible addition-fragmentation chain-transfer (RAFT)-mediated process. GOD was then covalently immobilized through the ring-opening reaction between the amine groups of the GOD and the epoxide groups of the grafted GMA polymer chains. X-ray photoelectron spectroscopy (XPS) was used to characterize the surface-modified surface after each modification stage. Increasing the thickness of the polymer layer and the immobilization time could allow a great amount of GOD to be immobilized on the silicon surface. The GOD-functionalized silicon hybrids are promising candidates for the silicon-based glucose biosensors.

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Enzyme Immobilization on Porous Silicon Surfaces

Cited by 114 publications

References 25 publications

Acetylcholinesterase immobilization and characterization, and comparison of the activity of the porous silicon-immobilized enzyme with its free counterpart

Acetylcholinesterase immobilization and characterization, and comparison of the activity of the porous silicon-immobilized enzyme with its free counterpart

Assessing the axonal translocation of CeO2 and SiO2 nanoparticles in the sciatic nerve fibers of the frog: an ex vivo electrophysiological study

Covalent Immobilization of Glucose Oxidase on Well-Defined Polymer-Silicon Wafer Hybrids via Surface-Initiated Raft-Mediated Process

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