2016
DOI: 10.1042/bsr20150154
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Abstract: SynopsisA successful prescription is presented for acetylcholinesterase physically adsorbed on to a mesoporous silicon surface, with a promising hydrolytic response towards acetylthiocholine iodide. The catalytic behaviour of the immobilized enzyme was assessed by spectrophotometric bioassay using neostigmine methyl sulfate as a standard acetycholinesterase inhibitor. The surface modification was studied through field emission SEM, Fourier transform IR spectroscopy, energy-dispersive X-ray spectroscopy, cathod… Show more

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Cited by 24 publications
(14 citation statements)
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References 63 publications
(77 reference statements)
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“…A. Similar pH values were found to be at pH 8 for AChE immobilized on mesoporous silicon surface , on SiO 2 hybrid membranes , on styrene–maleic anhydride copolymer , and acrylonitrile copolymer membranes .…”
Section: Resultssupporting
confidence: 61%
See 1 more Smart Citation
“…A. Similar pH values were found to be at pH 8 for AChE immobilized on mesoporous silicon surface , on SiO 2 hybrid membranes , on styrene–maleic anhydride copolymer , and acrylonitrile copolymer membranes .…”
Section: Resultssupporting
confidence: 61%
“…5A. Similar pH values were found to be at pH 8 for AChE immobilized on mesoporous silicon surface [31], on SiO 2 hybrid membranes [32], on styrene-maleic anhydride copolymer [33], and acrylonitrile copolymer membranes [7]. The influence of temperature on the activity of the free and immobilized AChE was investigated by varying the temperature from 20 to 40°C and the enzyme activity behaviors were demonstrated in Fig.…”
Section: Effect Of Ph and Temperature On Immobilized And Free Achesupporting
confidence: 62%
“…Studies have reported similar results for enzyme immobilized by physical adsorption methods (Chaudhari et al 2005;Saleem et al 2016). The change in the appearance of PS surface observed by SEM confirms the presence of enzyme molecules on PS film.…”
Section: Physical Adsorptionsupporting
confidence: 69%
“…[6]. Оптические свойства [7,8] пористого кремния были успешно использованы для изготовления датчиков и биосенсоров: например, мезопористый кремний использовался в качестве многоразового биокаталитического устройства [9]. Мезо-и наночастицы кремния биоразлагаемы и биосовместимы [10], являются отличными кандидатами для биосенсоров in vivo [11] и позволяют проводить in vitro диагностику раковых заболеваний на ранних стадиях [12].…”
Section: Introductionunclassified