Endophytic bacteria from Ethiopian coffee plants and their potential to antagonise Meloidogyne incognita

Mekete, Tesfamariam; Hallmann, Johannes; Kiewnick, Sebastian; Sikora, R. A.

doi:10.1163/156854108x398462

Cited by 58 publications

(31 citation statements)

References 25 publications

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“…untuk mengendalikan nematoda Meloidogyne sp. juga telah dilaporkan oleh beberapa peneliti (Mekete, Hallmann, Sebastian, & Sikora, 2009;Vetrivelkalai, Sivakumar, & Jonathan, 2010;Harni & Munif, 2012;Lamovsek, Urek, & Trdan, 2013;Munif, Hallmann, & Sikora, 2013). Mekete, Hallman, Sebastian, & Sikora (2009), melaporkan bakteri endofit Bacillus pumilus dan B. mycoides dapat menekan populasi dan jumlah puru Meloidogyne incognita pada tanaman kopi sebesar 33% dan 39%.…”

Section: Pendahuluanunclassified

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Pengaruh Formula Bionematisida Bakteri Endofit Bacillus sp. terhadap Infeksi Nematoda Meloidogyne sp. pada Tanaman Kopi

Harni

Samsudin

2015

JTIDP

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ABSTRAKNematoda puru akar (Meloidogyne sp.) merupakan salah satu pembatas produksi pada tanaman kopi. Pengendalian nematoda yang banyak dilakukan saat ini adalah menggunakan agens hayati seperti bakteri Bacillus sp. karena ramah lingkungan dan dapat meningkatkan pertumbuhan tanaman. Penelitian bertujuan mengetahui pengaruh bakteri endofit Bacillus sp. PG76 dalam bentuk formula molase, kompos, dan talc terhadap infeksi nematoda puru akar (Meloidogyne sp.) dan pertumbuhan tanaman kopi. Percobaan dilakukan di laboratorium dan rumah kaca Balai Penelitian Tanaman Industri dan Penyegar, Sukabumi mulai Desember 2013 sampai Mei 2014. Rancangan yang digunakan adalah rancangan acak lengkap 6 perlakuan dengan 10 ulangan. Perlakuannya adalah tiga formula bionematisida berupa molase, kompos, dan talc; nematisida kimia sebagai pembanding (karbofuran), kontrol positif (tanaman diinokulasi nematoda, tanpa formula), dan kontrol negatif (tanaman tanpa formula dan nematoda). Formula berisi bakteri endofit Bacillus sp. PG76 dengan kerapatan 10 9 cfu/ml. Pengujian formula dilakukan pada tanaman kopi berumur 6 bulan dengan konsentrasi 100 ml/pohon untuk molase, dan 100 g/pohon untuk kompos dan talc. Satu minggu setelah perlakuan formula, tanaman kopi diinokulasi dengan 500 ekor larva 2 Meloidogyne sp. Tiga bulan setelah perlakuan dilakukan pengamatan terhadap jumlah puru, populasi nematoda di dalam akar dan tanah serta pertumbuhan tanaman kopi. Hasil penelitian menunjukkan ketiga formula bionematisida Bacillus sp. PG76 (molase, kompos, dan talc) dapat menekan populasi nematoda Meloidogyne sp. pada tanaman kopi. Formula bionematisida terbaik adalah molase dan kompos dengan penekanan 74,0% dan 73,2%, sama efektifnya dengan nematisida kimia karbofuran (73,3%). Formula molase dan kompos juga dapat meningkatkan pertumbuhan tanaman kopi. Bionematisida mengandung Bacillus PG76 prospektif mengendalikan nematoda.

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Section: Pendahuluanunclassified

“…Perbanyakan nematoda Meloidogyne sp. dilakukan pada tanaman tomat (Mekete et al, 2009). Nematoda Meloidogyne sp.…”

Section: Perbanyakan Materi Tanaman Dan Nematodaunclassified

Pengaruh Formula Bionematisida Bakteri Endofit Bacillus sp. terhadap Infeksi Nematoda Meloidogyne sp. pada Tanaman Kopi

Harni

Samsudin

2015

JTIDP

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“…The densities of bacterial population obtained were similar to those previously reported. On coffee, the endophytic bacteria population densites varied from 5.2 x 10 2 to 2.07 x 10 6 cfu g -1 wet root (Mekete et al 2009). On sweet corn, the popu- (Hallmann et al 1997) and on potato was 10 3 cfu g -1 root (Krechel et al 2002).…”

Section: Exploration Of Endophytic Bacteriamentioning

confidence: 99%

“…temperature and rainfall), cultivation technique and soil amendment (Hallmann et al 1999;Garbeva et al 2004;Berg and Hallmann, 2006;Hallmann 2001;Zinniel et al 2002). Mekete et al (2009) reported that cultivation techniques greatly affect endophytic bacterial population on coffee plants. Endophytic bacterial population densities in semi-forest and forest coffee were higher than those observed in large-scale coffee plantations.…”

Section: Exploration Of Endophytic Bacteriamentioning

confidence: 99%

POTENTIAL USE OF ENDOPHYTIC BACTERIA TO CONTROL Pratylenchus brachyurus ON PATCHOULI

Harni

Supramana

Supriadi

2012

Indones. J. Agric. Sci.

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Pratylenchus brachyurus is an important parasitic nematode which significantly decreases quality and quantity of patchouli oil. One potential measure for controlling the nematode is by using endophytic bacteria. These bacteria also induce plant growth. This study aimed to evaluate the potential of endophytic bacteria to control P. brachyurus. The experiments were carried out in the Bacteriological Laboratory of the Plant Protection Department, Bogor Agricultural University, and the Laboratory and Greenhouse of the Indonesian Spice and Medicinal Crops Research Institute from April to December 2007. Endophytic bacteria were isolated from the roots of patchouli plants sampled from various locations in West Java. Antagonistic activity of the isolates were selected against P. brachyurus and their abilities to induce plant growth of patchouli plants. Isolates having ability to control P. brachyurus and promote plant growth were identified by molecular techniques using 16S rRNA universal primers. The results showed that a total of 257 isolates of endophytic bacteria were obtained from patchouli roots and their population density varied from 2.3 x 10 2 to 6.0 x 10 5 cfu g -1 fresh root. As many as 60 isolates (23.34%) were antagonistic against P. brachyurus causing 70-100% mortality of the namatode, 72 isolates (28.01%) stimulated plant growth, 32 isolates (12.47%) inhibited plant growth, and 93 isolates (36.18%) were neutral. Based on their antagonistic and plant growth enhancer characters, five isolates of the bacteria, namely Achromobacter xylosoxidans TT2, Alcaligenes faecalis NJ16, Pseudomonas putida EH11, Bacillus cereus MSK, and Bacillus subtilis NJ57 suppressed 74.0-81.6% nematode population and increased 46.97-86.79% plant growth. The study implies that the endophytic bacteria isolated from patchouly roots are good candidates for controlling P. brachyurus on patchouly plants.

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“…Similarly, B. pumilus reduced the gall formation and reproduction of the root-knot nematode (Akhtar and Siddiqui, 2008). Several studies have reported the nematicidal effect of B. pumilus on root-knot nematodes in different agricultural crops (Pinho et al, 2009;Mekete et al, 2009: Oliveira et al, 2007. However, information on active metabolites isolated form B. pumilus and its nematicidal activity have not yet been investigated.…”

Section: Introductionmentioning

confidence: 99%

Identification of 2-methylbutyric Acid as a Nematicidal Metabolite, and Biocontrol and Biofertilization Potentials of Bacillus pumilus L1

Lee¹,

Cho²,

Moon³

et al. 2016

Korean Journal of Soil Science and Fertilizer

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The present study described the isolation of 2-methylbutyric acid (2-MBA) produced from Bacillus pumilus L1, to subsequently investigate its nematicidal activity for the control of the root-knot nematode. The results showed that 2-MBA could be purified by chromatographic techniques and was identified using nuclear magnetic resonance and liquid chromatography-mass spectrometry. Crude extract and partially purified compounds had a significant effect on the inhibition of egg hatchability and second-stage juvenile (J2) mortality. A dose-dependent effect of 2-MBA was observed for J2 mortality and egg hatchability. Egg hatchability was 69.2%, 59.9%, 32.7%, and 0.0% at 125, 250, 500, and 1000 μg mL -1 of 2-MBA after 4 d of incubation, respectively. Meanwhile, J2 mortality was in the range of 24.4%-100.0% after 2 d of incubation, depending on the concentrations of 2-MBA used. A pot experiment also demonstrated that treatment of B. pumilus L1 culture caused a significant reduction in the number of galls, egg masses, and J2 population than that of the tap water (TW) control. However, as the B. pumilus L1 culture concentration was decreased, the efficacy of nematode control by treatment of B. pumilus L1 culture was reduced compared to that of TW. B. pumilus L1 inoculation at different concentrations also promoted cucumber plant growth. Therefore, our study demonstrated the potential of 2-MBA from B. pumilus L1 as a biocontrol agent against the root-knot nematode and a plant growth promoter for cucumber plants.

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Endophytic bacteria from Ethiopian coffee plants and their potential to antagonise Meloidogyne incognita

Cited by 58 publications

References 25 publications

Pengaruh Formula Bionematisida Bakteri Endofit Bacillus sp. terhadap Infeksi Nematoda Meloidogyne sp. pada Tanaman Kopi

Pengaruh Formula Bionematisida Bakteri Endofit Bacillus sp. terhadap Infeksi Nematoda Meloidogyne sp. pada Tanaman Kopi

POTENTIAL USE OF ENDOPHYTIC BACTERIA TO CONTROL Pratylenchus brachyurus ON PATCHOULI

Identification of 2-methylbutyric Acid as a Nematicidal Metabolite, and Biocontrol and Biofertilization Potentials of Bacillus pumilus L1

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