Electrochemical Sensor and Biosensor Strategies for ROS/RNS Detection in Biological Systems

Abstract

International audienceThis review summarises the main electrochemical sensors and biosensors developed for assessing the reactive oxygen (ROS) and nitrogen (RNS) species of interest in clinical and physiological analysis. Among these species, a special attention is focused on sensors for the detection of the highly reactive superoxide radicals O2.−, the very stable hydrogen peroxide H2O2, as well as the well-known RNS nitric oxide (NO.). The different methods adopted for the detection of each species are commented, considering both sensor and biosensors detection strategies

Seaweed polysaccharides and their potential biomedical applications

“…The biological activities of carrageenan have been tested for the treatment of respiratory weakness, pendamic H1N1 influenza strain, dengue, Hepatitis A, and African swine fever viruses . Superoxidase dismutase biosensor which is modulated by the κ‐carragenan gel membrane have been reported use to test scavenging properties of commercial drugs and also in the fabrication of microbial time temperature indicator .…”

Oxygen-tolerant proton reduction catalysis: much O2 about nothing?

“…This technique requires a disk electrode, consisting of the catalyst to be studied, encircled by an electrode ring, which is typically Pt. 36 ROS detection can also be achieved through the measurement of a unique spectroscopic signal, such as the distinct UV/vis peak of H 2 O 2 37 and mass-spectrometry allows the quantification of 18 O 2 reduction to H 2 18 O. Alternatively, spectroscopic probes can be used, which can specifically determine nM concentrations of a given ROS. 20 By holding the ring at oxidizing potentials with a bipotentiostat, it is possible to detect products from O 2 and H + reduction through their unique redox potentials.…”

Macroporous mesh of nanoporous gold in electrochemical monitoring of superoxide release from skeletal muscle cells

“…Biosensors are useful in numerous applications in medicine, including biomolecular sensing, monitoring cellular interactions with their microenvironment, and in assessing free radicals such as reactive oxygen and reactive nitrogen species (ROS and RNS) (Calas-Blanchard et al 2014;Ingebrandt 2015;Qiu et al 2014;Zhao et al 2011). Detection of antioxidants, free radicals, and particularly ROS have recently attracted much attention because of their diverse physiological and pathological effects (Halliwell 2006).…”