Macroporous mesh of nanoporous gold in electrochemical monitoring of superoxide release from skeletal muscle cells

Sadeghian, Ramin Banan; Han, Jonghee; Ostrovidov, Serge; Salehi, Sahar; Bahraminejad, Behzad; Ahadian, Samad; Chen, Luyang; Khademhosseini, Ali

doi:10.1016/j.bios.2016.06.067

Cited by 26 publications

(21 citation statements)

References 34 publications

(30 reference statements)

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“…The two types of biosensors identified, optical and electrochemical, were biocompatible and reliable because cells were able to adhere, grow and proliferate and were able to monitor cell differentiation through the evaluation of ALP fluctuation, FAK, Src, GTPase, and PKA activities, Ca concentration, cadherin and BGP production (Carvalho et al, ; Chung et al, ; Desai et al, ; Kuo et al, ; Liao et al, , ; Tay et al, ; Wan et al, ; Wiraja et al, ; Xu et al, ; Yeo et al, ; Zhang et al, ; Zhao et al, ). Also, the impedance produced by cells during their differentiation, the production of ATP during stretching and superoxide concentration are also efficiently monitored (Bagnaninchi & Drummond, ; Banan Sadeghian et al, ; Chiu et al, ; Hecht et al, ; Hildebrandt et al, ; Ižák et al, ; Reitinger et al, ).…”

Section: Resultsmentioning

confidence: 99%

Biosensors for real‐time monitoring of physiological processes in the musculoskeletal system: A systematic review

Veronesi

Tschon

Visani

et al. 2019

Journal Cellular Physiology

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Biosensors are composed of (bio)receptors, transducers, and detection systems and are able to convert the biological stimulus into a measurable signal.This systematic review evaluates the current state of the art of innovation and research in this field, identifying the biosensors that in vitro monitor the musculoskeletal system cellular processes.Two databases found 20 in vitro studies, from January 1, 2008 to December 31, 2017, dealing with musculoskeletal system cells. The biosensors were divided into two groups based on the transduction mechanism: optical or electrochemical.The first group evaluated osteoblasts or mesenchymal stem cell (MSC) biocompatibility, viability, differentiation, alkaline phosphatase, enzyme, and protein detection.The second group detected cell impedance, ATP release, and superoxide concentration in tenocytes, osteoblasts, MSCs, and myoblasts.This review highlighted that the in vitro scenario is still at an early phase and limited for what concerns both the type of bioanalyte and for the type of system detector used.

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Section: Resultsmentioning

confidence: 99%

Biosensors for real‐time monitoring of physiological processes in the musculoskeletal system: A systematic review

Veronesi

Tschon

Visani

et al. 2019

Journal Cellular Physiology

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“…Fc in the hydrogel matrix also facilitated the redox-stable states in the electrochemical biosensor. Consequently, the developed electrochemical biosensor successfully detected O 2 via an electrochemical method, Sadeghian et al [107] developed an innovative working electrode with a 3D macroporous mesh of nanoporous gold, as shown in Figure 6c. Figure 6a,b shows the success of the synthesis protocol.…”

Section: Electrochemical Methods For Superoxide Radical (O •−mentioning

confidence: 99%

Detection Technologies for Reactive Oxygen Species: Fluorescence and Electrochemical Methods and Their Applications

et al. 2021

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Reactive oxygen species (ROS) have been found in plants, mammals, and natural environmental processes. The presence of ROS in mammals has been linked to the development of severe diseases, such as diabetes, cancer, tumors, and several neurodegenerative conditions. The most common ROS involved in human health are superoxide (O2•−), hydrogen peroxide (H2O2), and hydroxyl radicals (•OH). Organic and inorganic molecules have been integrated with various methods to detect and monitor ROS for understanding the effect of their presence and concentration on diseases caused by oxidative stress. Among several techniques, fluorescence and electrochemical methods have been recently developed and employed for the detection of ROS. This literature review intends to critically discuss the development of these techniques to date, as well as their application for in vitro and in vivo ROS detection regarding free-radical-related diseases. Moreover, important insights into and further steps for using fluorescence and electrochemical methods in the detection of ROS are presented.

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“…used cytochrome c‐functionalized NPG to modify the working electrodes for monitoring the release of superoxide from skeletal muscle cells. [ 70 ] The device shows significantly improved superoxide sensitivity of 7.29 nA nM −1 cm −2 and reduced detection level of 70 pM. [ 70 ] This sensitivity is higher than that of any similar enzyme‐based electrochemical superoxide sensor, and satisfies the requirements for superoxide monitoring at the cell level.…”

Section: Biosensorsmentioning

confidence: 99%

“…[ 70 ] The device shows significantly improved superoxide sensitivity of 7.29 nA nM −1 cm −2 and reduced detection level of 70 pM. [ 70 ] This sensitivity is higher than that of any similar enzyme‐based electrochemical superoxide sensor, and satisfies the requirements for superoxide monitoring at the cell level. Heme is the active center of cytochrome c. Therefore, other heme‐containing enzymes or proteins also have the ability to detect H 2 O 2 .…”

Section: Biosensorsmentioning

confidence: 99%

Nanoporous gold: A review and potentials in biotechnological and biomedical applications

Xiao

Wang

et al. 2021

Nano Select

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Nanoporous gold (NPG) is one of the representatives of emerging materials that combine the advantages of nano, porous, and metallic materials. In addition to the general characteristics of nanoporous materials, NPG also has many unique properties, such as excellent structural stability, adjustable pore size, high conductivity, remarkable biocompatibility, clean surface, easy recycle and reuse, and unique electrochemical catalytic activities. These performances endow NPG promising application potentials in the fields of catalysis, environmental monitoring, energy storage, biomedicine, etc. Herein, this review summarizes the recent advances of NPG over the past decade. Besides preparation and modification of NPG, special emphasis is placed on its applications in biotechnology and biomedicine: catalysis, biosensors, biofuel cells, and drug delivery. The significant and representative studies in each area are comprehensively reviewed and analyzed as a reference for researchers working in related areas. The main challenges and future opportunities of NPG in these areas are also discussed.

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Macroporous mesh of nanoporous gold in electrochemical monitoring of superoxide release from skeletal muscle cells

Cited by 26 publications

References 34 publications

Biosensors for real‐time monitoring of physiological processes in the musculoskeletal system: A systematic review

Biosensors for real‐time monitoring of physiological processes in the musculoskeletal system: A systematic review

Detection Technologies for Reactive Oxygen Species: Fluorescence and Electrochemical Methods and Their Applications

Nanoporous gold: A review and potentials in biotechnological and biomedical applications

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