Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis

Tabata, Miyuki; Yang, Huiyong; Mannan, Fahmida; Katayama, Yurika; Goda, Tatsuro; Matsumoto, Akira; Seichi, Ayaka; Suzuki, Kumi; Miyahara, Yuji

doi:10.1109/transducers.2015.7181234

Cited by 3 publications

(1 citation statement)

References 11 publications

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“…In addition, IROF pH electrodes can also be used to determine the pH of biological samples and tissue culture media and for realtime monitoring of pH changes in culture media to more objectively and accurately assess the biological condition of cultures [66,67]. Tabata et al successfully monitored proton release during an amplification reaction in real-time, using a miniature pH electrode, and demonstrated the quantitative detection of nucleic acids [68]. Vanhoudt et al reduced a mmscale to the pm-scale by anodic polarization and protected the iridium pH electrode by cyclic voltammetry in 0.5 M sulfuric acid by placing silk in a capillary glass tube [41].…”

Section: Marine Organismsmentioning

confidence: 99%

pH electrodes based on iridium oxide films for marine monitoring

Zhou

Pan

et al. 2020

Trends in Environmental Analytical Chemistry

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The pH is an important parameter that affects the growth and development of marine organisms, environmental changes, and industrial and agricultural production processes. Nowadays, important trends in pH detection and analysis are higher stability, adaptation to extreme environmental conditions, miniaturization, portability, and digital intelligence. Several studies have focused on the application of the iridium oxide film (IROF) pH electrodes in water quality monitoring and physiological analysis. The central aim of this work was to review the preparation techniques of the IROF pH electrodes and to expand their application in the field of marine monitoring. The studied methods include electrochemical deposition, electrochemical growth, sputtering deposition, heat treatment, and novel preparation methods. The IROF pH electrodes prepared via these methods are more sensitive, have a wider pH measurement ranges, and can be miniaturized further than traditional glass and pH photometer. Hence, in environmental analysis, combining IROF pH electrodes with wireless technology for the physiological and biochemical analysis of marine organisms, seawater, and sediment pore water is an important development tendency.

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Section: Marine Organismsmentioning

confidence: 99%

pH electrodes based on iridium oxide films for marine monitoring

Zhou

Pan

et al. 2020

Trends in Environmental Analytical Chemistry

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A Reconfigurable, Pulse-shaping Potentiometric Readout System for Bio-Sensing Transistors

Shan

Yang

et al. 2019

2019 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)

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Iridium Oxide Enabled Sensors Applications

Dong

Sun

2021

Catalysts

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There have been numerous studies applying iridium oxides in different applications to explore their proton-change-based reactions since the 1980s. Iridium oxide can be fabricated directly by applying electrodeposition, sputter-coating method, or oxidation of iridium wire. Generally, there have been currently two approaches in applying iridium oxide to enable its sensing applications. One was to improve or create different electrolytes with (non-)electrodeposition method for better performance of Nernst Constant with the temperature-related system. The mechanism behind the scenes were summarized herein. The other was to change the structure of iridium oxide through different kinds of templates such as photolithography patterns, or template-assisted direct growth methods, etc. to improve the sensing performance. The detection targets varied widely from intracellular cell pH, glucose in an artificial sample or actual urine sample, and the hydrogen peroxide, glutamate or organophosphate pesticides, metal-ions, etc. This review paper has focused on the mechanism of electrodeposition of iridium oxide in aqueous conditions and the sensing applications towards different biomolecules compounds. Finally, we summarize future trends on Iridium oxide based sensing and predict future work that could be further explored.

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Electrochemical real-time monitoring of isothermal nucleic acid amplification for quantitative analysis

Cited by 3 publications

References 11 publications

pH electrodes based on iridium oxide films for marine monitoring

pH electrodes based on iridium oxide films for marine monitoring

A Reconfigurable, Pulse-shaping Potentiometric Readout System for Bio-Sensing Transistors

Iridium Oxide Enabled Sensors Applications

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