A Reconfigurable, Pulse-shaping Potentiometric Readout System for Bio-Sensing Transistors

Lu, Shao-Yung; Shan, Siang-Sin; Yang, Jiancheng; Chang, C. Y.; Ren, F.; Lin, Jenshan; Pearton, S. J.; Liao, Yu-Te

doi:10.1109/embc.2019.8857404

2019 41st Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC) 2019

DOI: 10.1109/embc.2019.8857404

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A Reconfigurable, Pulse-shaping Potentiometric Readout System for Bio-Sensing Transistors

Shao-Yung Lu

Siang-Sin Shan

Jiancheng Yang

et al.

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Cited by 5 publications

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“…10 A double-pulse gating method was implemented to eliminate undesirable screening effects and charge accumulation. 11 Various targeted microbes such as the Zika virus, cerebrospinal fluid, and cardiac troponin I have all been detected with the same approach. [12][13][14] The objective of this study was to determine the possible impact of certain microorganisms in disrupting the ability of a novel sensor to detect the SARS-CoV2 virus in artificial saliva, thereby causing an interference.…”

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confidence: 99%

Analytical Specificity and Microbial Interference Study of a 30-Second Quantitative SARS-CoV-2 Detection Biosensor System

Chiang

Chiu

Ren

et al. 2022

ECS J. Solid State Sci. Technol.

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The analytical specificity and microbial interference of a SARS-CoV-2 biosensor detection platform are elucidated. A cost-effective and highly sensitive detection system for the virus has been developed with the capability of producing quantitative results comparable with polymerase chain reaction (PCR) within 30 seconds. This could meet the demand for a fast diagnosis solution needed for the ongoing global pandemic. Disposable strips were biofunctionalized and immobilized with monoclonal SARS-CoV-2 antibodies. A printed circuit board embedded with a metal–oxide–semiconductor field-effect transistor (MOSFET) was also designed. The strips were connected to the gate electrode of the MOSFET, which received a synchronous pulse along with the drain electrode. The resulting waveform from the drain was then converted to digital readouts corresponding to virus or spike protein concentrations. We investigated 26 common organisms which are likely presented in the respiratory system along with 5 pathogens from the same genetic family of the SARS-CoV-2 virus for having cross-reaction or microbial interference, either of which would hinder the efficacy of the system. None of these organisms decreased the virus detection effectiveness of the sensor system.

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mentioning

confidence: 99%

Analytical Specificity and Microbial Interference Study of a 30-Second Quantitative SARS-CoV-2 Detection Biosensor System

Chiang

Chiu

Ren

et al. 2022

ECS J. Solid State Sci. Technol.

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A Review of CMOS Electrochemical Readout Interface Designs for Biomedical Assays

Shan

et al. 2021

IEEE Sensors J.

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Fast SARS-CoV-2 virus detection using disposable cartridge strips and a semiconductor-based biosensor platform

Xian

Luo

Xia

et al. 2021

Journal of Vacuum Science &Amp; Technology B, Nanotechnology and Microelectronics: Materials, Processing, Measurement, and Phen

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Detection of the SARS-CoV-2 spike protein and inactivated virus was achieved using disposable and biofunctionalized functional strips, which can be connected externally to a reusable printed circuit board for signal amplification with an embedded metal–oxide–semiconductor field-effect transistor (MOSFET). A series of chemical reactions was performed to immobilize both a monoclonal antibody and a polyclonal antibody onto the Au-plated electrode used as the sensing surface. An important step in the biofunctionalization, namely, the formation of Au-plated clusters on the sensor strips, was verified by scanning electron microscopy, as well as electrical measurements, to confirm successful binding of thiol groups on this Au surface. The functionalized sensor was externally connected to the gate electrode of the MOSFET, and synchronous pulses were applied to both the sensing strip and the drain contact of the MOSFET. The resulting changes in the dynamics of drain waveforms were converted into analog voltages and digital readouts, which correlate with the concentration of proteins and virus present in the tested solution. A broad range of protein concentrations from 1 fg/ml to 10 μg/ml and virus concentrations from 100 to 2500 PFU/ml were detectable for the sensor functionalized with both antibodies. The results show the potential of this approach for the development of a portable, low-cost, and disposable cartridge sensor system for point-of-care detection of viral diseases.

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A Reconfigurable, Pulse-shaping Potentiometric Readout System for Bio-Sensing Transistors

Cited by 5 publications

References 11 publications

Analytical Specificity and Microbial Interference Study of a 30-Second Quantitative SARS-CoV-2 Detection Biosensor System

Analytical Specificity and Microbial Interference Study of a 30-Second Quantitative SARS-CoV-2 Detection Biosensor System

A Review of CMOS Electrochemical Readout Interface Designs for Biomedical Assays

Fast SARS-CoV-2 virus detection using disposable cartridge strips and a semiconductor-based biosensor platform

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