Electro-acupuncture upregulates CGRP expression after rat spinal cord transection

Li, Wen Jie; Li, Shu Min; Ding, Ying; He, Bing; Keegan, Jack; Dong, Hongxin; Ruan, Jing Wen; Zeng, Yuan

doi:10.1016/j.neuint.2012.10.002

Cited by 29 publications

(29 citation statements)

References 35 publications

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“…As it is well known that dorsal root ganglion neurons project to the spinal cord, where morphological plasticity is present after dorsal root ganglion removal [49]and could also explain the neurotrophin action, it is possible that the recovery of motor function observed in the present study could be due to the induction NGF, BDNF and NT-3 expression by EA. Moreover, it has been demonstrated that EA can promote the differentiation of mesenchymal stem cells and the regeneration of nerve fibers in injured spinal cord through induction of endogenous NT-3 and that the combination of EA and mesenchymal stem cellstransplantation can partially improve the function of paralyzed hind limbs [50].…”

Section: Discussionmentioning

confidence: 57%

Electro-Acupuncture at GV.4 Improves Functional Recovery in paralyzed rats after a Traumatic Spinal Cord Injury

O¹,

H²,

C³

et al. 2016

acupunct electrother res

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In the present study, the effect of electro-acupuncture (EA) on the oxidative stress, the spinal cord tissue preservation and the recovery of motor function was evaluated after a traumatic spinal cord injury (TSCI). Long Evans rats were randomized into five groups: 1. Sham; 2. TSCI without treatment; 3. TSCI + EA (acupoint GV.4); 4. TSCI + EA (acupoint GV.26) and 5.TSCI + EA (GV.4 + GV.26). The EA was performed with an Electro-Acupunctoscope, AWQ-104L Digital, wave dense-dispersed, current intensity 2.5mA and frequency 2-100Hz for 30 minutes. The biochemical results showed a significant increase in the hydroxyl radical concentration in group 2 (3.1±1.4nmol) compared with groups 1 (1.8±0.5nmol) and 4 (2.4±1.1nmol) (p<0.05), whereas in group 4 (4.8±1.8nmol), there was a significant increase in lipid peroxidation when compared with group 1 (1.7±0.5nmol) (p<0.05). The BBB motor function score in the paralyzed hind limbs (normal BBB=21points) was greater in groups 3 (15.2 points) and 5 (13.5 points) in comparison with groups 2 (11.4 points) and 4 (9.3 points) (p<0.05). The quantity of preserved spinal cord tissue was greater in group 3 (6582.7±20µm 2 ) than in groups 2 (5262.4±20µm 2 ), 4 (3995.6±26µm 2 ) and 5 (4266.7±22µm 2 ). Although EA in GV.26 decreases hydroxyl radical concentration (50%), it significantly increases lipid peroxidation (45%), while stimulation of GV.4 decreases oxidative stress (15%), preserves spinal cord tissue (25%) and improves recovery of motor function 356 kARATAY, L.; UzkESER, z. 1 in the hind limbs of rats with paralysis (18.1%) compared with untreated group. These findings suggest that EA in GV.4 may be a therapeutic alternative on TSCI.

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Section: Discussionmentioning

confidence: 57%

Electro-Acupuncture at GV.4 Improves Functional Recovery in paralyzed rats after a Traumatic Spinal Cord Injury

O¹,

H²,

C³

et al. 2016

acupunct electrother res

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“…EA can induce the regeneration of CGRP-positive nerve fibers in SCI rats (Yan et al, 2011[38]). EA can also increase the expression of CGRP in rat spinal cord transaction, and thereby improve the survival of damaged neurons and prevent the loss of neurons (Li et al, 2012[24]). Nevertheless, the molecular mechanism underlying the therapeutic effect of EA on SCI has not been fully clarified.…”

Section: Introductionmentioning

confidence: 99%

Electro-acupuncture promotes the proliferation of neural stem cells and the survival of neurons by downregulating miR-449a in rat with spinal cord injury

Zhu

Zhang³

2017

EXCLI Journal; 16:Doc363; ISSN 1611-2156

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“…10 Solá et al found that EA at the Jaji points at thoracic levels 7 and 8 significantly reduced SCI caused by myocardial ischaemiareperfusion, possibly by the upregulation of Bcl-2 protein expression and downregulation of Bax protein expression, thus increasing the Bcl-2/Bax ratio and thereby inhibiting myocardial apoptosis. 11 In addition, Bleicken et al 12 and Li et al 13 found that acupuncture significantly reduced neuronal apoptosis and promoted nerve repair and regeneration, playing a neuroprotective role in cerebral ischaemia. Collectively, the previous studies suggest that EA has a regulatory role in neuronal apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Anti-Apoptotic Signal Transduction Mechanism of Electroacupuncture in Acute Spinal Cord Injury

Quan¹,

Chen²,

Du³

et al. 2014

Acupunct Med

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Spinal cord injury (SCI) can be caused by a variety of pathogenic factors. In China, acupuncture is widely used to treat SCI. We previously found that acupuncture can reduce apoptosis and promote repair after SCI. However, the antiapoptotic mechanisms by which acupuncture exerts its effects on SCI remain unclear. Our aim was to investigate the role of the PI3K/Akt and extracellular signal-regulated kinases (ERK)1/2 signalling pathways in acupuncture treatment of acute SCI. Eighty pure-bred New Zealand rabbits were randomly divided into the following five groups (n=16 per group): control; model; elongated needle electroacupuncture (EA); EA+LY294002; and EA+PD98059. We established a spinal cord contusion model of SCI in all experimental groups except controls, in which only a laminectomy was performed. After SCI, three of the groups received EA once daily for 3 days. One hour before SCI, the two drug groups received LY294002 (Akt inhibitor; 10 μg, 20 μL) or PD98059 (ERK inhibitor; 3 μg, 20 μL) via intrathecal injection. At 48 h after SCI, animals were killed and spinal cord tissue samples were collected for transferase dUTP nick end labelling (TUNEL) assays, immunohistochemistry and western blot assays. EA significantly increased p-Akt and p-ERK1/2 expression, reduced cytochrome c and caspase-3 expression and inhibited neuronal apoptosis in the injured spinal cord segment. The opposite effects were seen after using Akt and ERK inhibitors. Acupuncture promotes the repair of SCI, possibly by activation of the PI3K/Akt and ERK1/2 signalling pathways and by inhibition of the mitochondrial apoptotic pathway.

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Electro-acupuncture upregulates CGRP expression after rat spinal cord transection

Cited by 29 publications

References 35 publications

Electro-Acupuncture at GV.4 Improves Functional Recovery in paralyzed rats after a Traumatic Spinal Cord Injury

Electro-Acupuncture at GV.4 Improves Functional Recovery in paralyzed rats after a Traumatic Spinal Cord Injury

Electro-acupuncture promotes the proliferation of neural stem cells and the survival of neurons by downregulating miR-449a in rat with spinal cord injury

Anti-Apoptotic Signal Transduction Mechanism of Electroacupuncture in Acute Spinal Cord Injury

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