Biochar can play a key role in nutrient cycling, potentially affecting nitrogen retention when applied to soils. In this project, laboratory experiments were conducted to investigate the adsorption properties of bamboo charcoal (BC) and the influence of BC on nitrogen retention at different soil depths using multilayered soil columns. Results showed that BC could adsorb ammonium ion predominantly by cation exchange. Ammonium nitrogen (NH 4 + -N) concentrations in the leachate of the soil columns showed significant differences at different depths after ammonium chloride application to the columns depending on whether BC had been added. Addition of 0.5% BC to the surface soil layer retarded the downward transport of NH 4 + -N in the 70-day experiment, as indicated by measurements made during the first 7 days at 10 cm, and later, in the experimental period at 20 cm. In addition, application of BC reduced overall cumulative losses of NH 4 + -N via leaching at 20 cm by 15.2%. Data appeared to suggest that BC could be used as a potential nutrient-retaining additive in order to increase the utilization efficiency of chemical fertilizers. Nonetheless, the effect of BC addition on controlling soil nitrogen losses through leaching needs to be further assessed before large-scale applications to agricultural fields are implemented.
Three-dimensional (3D) cell culture plays an invaluable role in tumor biology by providing in vivo like microenviroment and responses to therapeutic agents. Among many established 3D scaffolds, hydrogels demonstrate a distinct property as matrics for 3D cell culture. Most of the existing pre-gel solutions are limited under physiological conditions such as undesirable pH or temperature. Here, we report a peptide hydrogel that shows superior physiological properties as an in vitro matrix for 3D cell culture. The 3D matrix can be accomplished by mixing a self-assembling peptide directly with a cell culture medium without any pH or temperature adjustment. Results of dynamic rheological studies showed that this hydrogel can be delivered multiple times via pipetting without permanently destroying the hydrogel architecture, indicating the deformability and remodeling ability of the hydrogel. Human epithelial cancer cells, MCF-7, are encapsulated homogeneously in the hydrogel matrix during hydrogelation. Compared with two-dimensional (2D) monolayer culture, cells residing in the hydrogel matrix grow as tumor-like clusters in 3D formation. Relevant parameters related to cell morphology, survival, proliferation, and apoptosis were analyzed using MCF-7 cells in 3D hydrogels. Interestingly, treatment of cisplatin, an anti-cancer drug, can cause a significant decrease of cell viability of MCF-7 clusters in hydrogels. The responses to cisplatin were dose- and time-dependent, indicating the potential usage of hydrogels for drug testing. Results of confocal microscopy and Western blotting showed that cells isolated from hydrogels are suitable for downstream proteomic analysis. The results provided evidence that this peptide hydrogel is a promising 3D cell culture material for drug testing.
The modern intelligent transportation system brings not only new opportunities for vehicular Internet of Things (IoT) services but also new challenges for vehicular ad-hoc networks (VANETs). Apart from enhanced network performance, a practical and reliable security scheme is needed to handle the trust management while preserving user privacy at the same time. The emerging 5G mobile communication system is viewed as a prominent technology for ultra-reliable, low-latency wireless communication services. Furthermore, incorporating software-defined network (SDN) architecture into the 5G-VANET enables global information gathering and network control. Hence, real-time IoT services on transportation monitoring and reporting can be well supported. Both pave the way for an innovative vehicular security scheme. This paper investigates the security and privacy issue in the transportation system and the vehicular IoT environment in SDN-enabled 5G-VANET. Due to the decentralized and immutable characteristics of blockchain, a blockchain-based security framework is designed to support the vehicular IoT services, i.e., real-time cloud-based video report and trust management on vehicular messages. This paper explicitly illustrates the SDN-enabled 5G-VANET model and the scheduling procedures of the blockchain-based framework. The numerical simulation results also show that malicious vehicular nodes or messages can be well detected while the overhead and impact on the network performance are acceptable for large-scale scenarios. Through case studies and theoretical analysis, we demonstrate our design substantially guarantees a secure and trustworthy vehicular IoT environment with user privacy preserved.
Background: Bone marrow mesenchymal stem cells (MSCs) are one of the potential tools for treatment of the spinal cord injury; however, the survival and differentiation of MSCs in an injured spinal cord still need to be improved. In the present study, we investigated whether Governor Vessel electro-acupuncture (EA) could efficiently promote bone marrow mesenchymal stem cells (MSCs) survival and differentiation, axonal regeneration and finally, functional recovery in the transected spinal cord.
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