Electro-acupuncture promotes the proliferation of neural stem cells and the survival of neurons by downregulating miR-449a in rat with spinal cord injury

Zhu, Yi; Wu, Ya‐Fen; Zhang, Rong

doi:10.17179/excli2017-123

Cited by 12 publications

(5 citation statements)

References 42 publications

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“…Acupuncture protects the nerves and reduces apoptosis of neurons and oligodendrocytes, thus improving functional recovery after SCI ( Cai and Shen, 2018 ). In addition, electroacupuncture can inhibit spinal cord neuronal apoptosis by increasing the Bcl-2 expression and inhibiting caspase-3 and Bax ( Zhao et al, 2008 ; Shi et al, 2016 ; Liu and Wu, 2017 ; Zhu et al, 2017 ).…”

Section: Mechanism Of Acupuncture Therapy In Spinal Cord Injurymentioning

confidence: 99%

“…The upregulation of exosomal miR-146b, NeuroD1, the activation of the Notch pathway, and the downregulation of the PTEN expression are associated with acupuncture-induced improvement of neurological injury after ischemic stroke ( Tao et al, 2014 ; Zhao et al, 2015 ; Sha et al, 2019 ; Zhang et al, 2020 ). In contrast, the potential signals of the acupuncture-induced NSC regulation in the SCI model include Wnt/β-catenin ( Zhang et al, 2017a ), ERK ( Xu et al, 2019 ), miR-449a ( Zhu et al, 2017 ), and Notch pathway ( Wang et al, 2021b ). Third, electroacupuncture reinforces the survival and synaptogenesis of transplanted NSC-derived neural network scaffolds as a neuronal relay bridging two severed ends of the injured spinal cord ( Jin et al, 2019 ).…”

Section: Mechanism Of Acupuncture Therapy In Spinal Cord Injurymentioning

confidence: 99%

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Mechanism Underlying Acupuncture Therapy in Spinal Cord Injury: A Narrative Overview of Preclinical Studies

Jiang¹,

Sun²,

Chen³

2022

Front. Pharmacol.

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Spinal cord injury (SCI) results from various pathogenic factors that destroy the normal structure and function of the spinal cord, subsequently causing sensory, motor, and autonomic nerve dysfunction. SCI is one of the most common causes of disability and death globally. It leads to severe physical and mental injury to patients and causes a substantial economic burden on families and the society. The pathological changes and underlying mechanisms within SCI involve oxidative stress, apoptosis, inflammation, etc. As a traditional therapy, acupuncture has a positive effect promoting the recovery of SCI. Acupuncture-induced neuroprotection includes several mechanisms such as reducing oxidative stress, inhibiting the inflammatory response and neuronal apoptosis, alleviating glial scar formation, promoting neural stem cell differentiation, and improving microcirculation within the injured area. Therefore, the recent studies exploring the mechanism of acupuncture therapy in SCI will help provide a theoretical basis for applying acupuncture and seeking a better treatment target and acupuncture approach for SCI patients.

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Section: Mechanism Of Acupuncture Therapy In Spinal Cord Injurymentioning

confidence: 99%

Section: Mechanism Of Acupuncture Therapy In Spinal Cord Injurymentioning

confidence: 99%

Mechanism Underlying Acupuncture Therapy in Spinal Cord Injury: A Narrative Overview of Preclinical Studies

Jiang¹,

Sun²,

Chen³

2022

Front. Pharmacol.

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“…As important regulators of normal nervous functions, miRNAs are closely related to morphological and functional changes in the nervous system [ 31 ]. Following a SCI, the homeostasis of many miRNAs is disturbed, and the aberrantly dysregulated miRNAs can either alleviate or aggravate conditions after an SCI, as dictated by their downstream target genes [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, autophagy can limit neuronal death by selectively reducing the abundance of proapoptotic proteins in the cytosol [ 4 ]. Moreover, it has been revealed that EA can contribute to functional recovery by attenuating SCI-induced Bax and Caspase-3 upregulation in injured spinal cords [ 13 , 31 ]. In this study, our data show that an EA-enhanced autophagy flux was accompanied by inhibition in apoptosis, manifested as a gradual decrease in Bax and Caspase-3 levels.…”

Section: Discussionmentioning

confidence: 99%

Electroacupuncture-Modulated MiR-106b-5p Expression Enhances Autophagy by Targeting Beclin-1 to Promote Motor Function Recovery After Spinal Cord Injury in Rats

Guo¹,

Chen²,

Yang³

et al. 2023

Neurospine

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Objective: Electroacupuncture (EA) has a definite effect on the treatment of spinal cord injuries (SCIs), but its underlying molecular mechanism remains unclear. Meanwhile, MiR106b-5p is an autophagy- and apoptosis-related microribonucleic acid, but whether it regulates the progression of autophagy and apoptosis in SCIs is yet undetermined. As such, this study aimed to elucidate the involvement of miR-106b-5p in the EA treatment of an SCI.Methods: The miR-106b-5p level was detected by quantitative real-time polymerase chain reaction. In vitro, SH-SY5Y cells were transfected with miR-106b-5p mimics or inhibitors to regulate the miR-106b-5p expression, while <i>in vivo</i>, SCI rats were treated with EA for 7 days at the bilateral Zusanli (ST36) and Jiaji (EX-B2) acupoints. The motor function was evaluated using the Basso-Beattie-Bresnahan (BBB) criteria. Further, autophagic vacuoles, pathological damage, and neuronal cell morphology were observed by transmission electron microscopy, as well as by hematoxylin and eosin and Nissl staining, respectively.Results: The miR-106b-5p level, which can interact directly with Beclin-1 by influencing its expression, as well as the expressions of P62, Caspase-3, and Bax, was upregulated after an SCI, but it decreased after EA. Moreover, the ratio of LC3-II to LC3-I was upregulated after EA. EA can enhance autophagy, reduce neuronal apoptosis, and minimize motor dysfunction and histopathological deficits after an SCI. More importantly, however, all the above effects induced by EA can be reversed after an injection of miR-106-5p agomir to produce an overexpression of miR-106b-5p.Conclusion: EA treatment could downregulate miR-106b-5p to alleviate SCI-mediated injuries by promoting autophagy and inhibiting apoptosis.

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“…miR-449a, located on chromosome 5q11, has been reported to be dysregulated in many diseases and adjust cell apoptosis, proliferation, autophagy and inflammation by influencing downstream genes expression [ 63 ]. miR-449a could induce inflammatory factors release in the spinal cord of spinal cord injury rats [ 64 ]. Also, miR-449a played a crucial role in the progression of pulmonary fibrogenesis [ 63 ].…”

Section: Discussionmentioning

confidence: 99%

Integrative analysis links autophagy to intrauterine adhesion and establishes autophagy-related circRNA-miRNA-mRNA regulatory network

Peng,

Zhu,

Wang

et al. 2023

Aging

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Background: Intrauterine adhesion (IUA) is a troublesome complication characterized with endometrial fibrosis after endometrial trauma. Increasing number of investigations focused on autophagy and noncoding RNA in the pathogenesis of uterine adhesion, but the underlying mechanism needs to be further studied. Methods: mRNA expression profile and miRNA expression profile were obtained from Gene Expression Omnibus database. The autophagy related genes were low. Venn diagram was used to set the intersection of autophagy genes and DEGs to obtain ARDEGs. Circbank was used to select hub autophagy-related circRNAs based on ARDEMs. Then, the differentially expressed autophagy-related genes, miRNAs and circRNAs were analyzed by functional enrichment analysis, and protein-protein interaction network analysis. Finally, the expression levels of hub circRNAs and hub miRNAs were validated through RT-PCR of clinical intrauterine adhesion samples. In vitro experiments were investigated to explore the effect of hub ARCs on cell autophagy, myofibroblast transformation and collagen deposition. Results: 11 autophagy-related differentially expressed genes (ARDEGs) and 41 differentially expressed miRNA (ARDEMs) compared between normal tissues and IUA were identified. Subsequently, the autophagy-related miRNA-mRNA network was constructed and hub ARDEMs were selected. Furthermore, the autophagy-related circRNA-miRNA-mRNA network was established. According to the ranking of number of regulated ARDEMs, hsa-circ-0047959, hsa-circ-0032438, hsa-circ-0047301 were regarded as the hub ARCs. In comparison of normal endometrial tissue, all three hub ARCs were upregulated in IUA tissue. All hub ARDEMs were downregulated except has-miR-320c. Conclusions: In the current study, we firstly constructed autophagy-related circRNA-miRNA-mRNA regulatory network and identified hub ARCs and ARDEMs had not been reported in IUA.

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Electro-acupuncture promotes the proliferation of neural stem cells and the survival of neurons by downregulating miR-449a in rat with spinal cord injury

Cited by 12 publications

References 42 publications

Mechanism Underlying Acupuncture Therapy in Spinal Cord Injury: A Narrative Overview of Preclinical Studies

Mechanism Underlying Acupuncture Therapy in Spinal Cord Injury: A Narrative Overview of Preclinical Studies

Electroacupuncture-Modulated MiR-106b-5p Expression Enhances Autophagy by Targeting Beclin-1 to Promote Motor Function Recovery After Spinal Cord Injury in Rats

Integrative analysis links autophagy to intrauterine adhesion and establishes autophagy-related circRNA-miRNA-mRNA regulatory network

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