Effects of selenium supplementation on the oxidative state of acute heat stress‐exposed quails

Abstract

This study aimed to evaluate the effect of heat stress (HS) and selenium supplementation on markers of stress, meat quality and gene expression. For this, meat quails of 42 days of age were fed a diet that either met [0.33 mg/kg, nutritional demand for selenium (SS)] or did not meet [0.11 mg/kg, selenium deficient (SD)] the nutritional demands for selenium during the 7 days of evaluation. In addition, the animals were kept at either a thermal comfort temperature (25 °C) or exposed to HS (38 °C for 24 h). Glutathione synthetase (GSS), glutathione reductase (GSR) and uncoupling protein (UCP) gene expression were influenced by the interaction between temperature and diet. Animals subjected to HS and fed the SS diet exhibited the highest GSS and GSR gene expression. In terms of UCP gene expression, the lowest values were observed in HS animals on the SD diet. Glutathione peroxidase 7 (GPX7) gene expression, body temperature (BT) and creatine kinase (CK) activity were influenced by both selenium supplementation and HS. Aspartate aminotransferase (AST), alanine aminotransferase (ALT) activity and creatinine content all were influenced by the diet/environment interaction. The highest AST activity, ALT activity and creatinine levels were observed in animals that were both on the SD diet and exposed to HS. HS animals also exhibited an increased heterophil/lymphocyte ratio and lower triiodothyronine (T3) hormone levels than birds that remained at the comfortable temperature. Animals subjected to HS and fed with selenium supplemented diet showed better results regarding gene expression and, thus, better results for the activities of enzymes used as stress markers, which could be due to the higher antioxidant capacity provided by the action of the studied genes.

Effects of dietary supplemental methionine source and betaine replacement on the growth performance and activity of mitochondrial respiratory chain enzymes in normal and heat‐stressed broiler chickens

“…In agreement with these results, Yang et al () inferred that Met restriction reduces Cox I activity in porcine liver mitochondria without affecting other complexes. As stated by Del Vesco et al (), ambient temperature and Met supplementation affect H 2 O 2 production such that in heat‐stressed quails and those fed a diet void of Met supplementation, a higher rate of H 2 O 2 production was observed. They suggested that under heat stress conditions where H 2 O 2 production was the highest, Met supplementation could attenuate ROS‐induced damage, possibly due to the increased antioxidant activity of GSH and glutathione peroxidase.…”

Polyphenols as Potential Attenuators of Heat Stress in Poultry Production

“…Pamok et al [38] reported that after four days of acute heat stress (38 ± 2 °C), the GSH-Px activity was increased together with serum malondialdehyde (MDA), which can reflect the degree of oxidative damage in poultry. Vesco et al [39] also reported that acute heat stress (38 °C for 24 h) increased the expression of the GSH-Px gene. However, chronic heat stress can break the antioxidant enzyme system and cause the ROS accumulation in the body to induce oxidative stress by decreasing the activity of CAT, SOD, and GSH-Px.…”

“…Glutathione peroxidase is a selenium-dependent enzyme, meaning more selenium is needed when the body is under heat stress. Lack of selenium will reduce the activity of GSH-Px [39].…”

A comparison of two sources of methionine supplemented at different levels on heat shock protein 70 expression and oxidative stress product of Peking ducks subjected to heat stress

“…Exposure to abiotic or biotic stress generates excessive concentrations of ROS, such as superoxide , hydrogen peroxide (H 2 O 2 ) and hydroxyl radicals (OH − ) (Murphy, ). Similarly, HS disturbed redox homoeostasis by enhancing the production of radical and causing oxidative damage of lipids, proteins and DNA (Del Vesco, Gasparino, Zancanela, Grieser, Guimaraes et al., ; Mujahid, ; Schrauwen, Schrauwen‐Hinderling, Hoeks, & Hesselink, ). MDA and protein carbonylation represented the oxidative damage metabolites of lipid and protein respectively.…”