We have developed a new DH mapping population for oilseed rape, named TNDH, using genetically and phenotypically diverse parental lines. We used the population in the construction of a high stringency genetic linkage map, consisting of 277 loci, for use in quantitative genetic analysis. A proportion of the markers had been used previously in the construction of linkage maps for Brassica species, thus permitting the alignment of maps. The map includes 68 newly developed Sequence Tagged Site (STS) markers targeted to the homologues of defined genes of A. thaliana. The use of these markers permits the alignment of our linkage map with the A. thaliana genome sequence. An additional 74 loci (31 newly developed STS markers and 43 loci defined by SSR and RFLP markers that had previously been used in published linkage maps) were added to the map. These markers increased the resolution of alignment of the newly constructed linkage map with existing Brassica linkage maps and the A. thaliana genome sequence. We conducted field trials with the TNDH population at two sites, and over 2 years, and identified reproducible QTL for seed oil content and erucic acid content. The results provide new insights into the genetic control of seed oil and erucic acid content in oilseed rape, and demonstrate the utility of the linkage map and population.
SummaryThe aim of this study was to examine the effects of different sources and levels of | INTRODUCTIONBirds were vulnerable to high temperatures during summer because of the insufficient heat dissipation after a meal, especially possible for waterfowl (e.g., duck). Heat stress (HS) badly effects on both immune function and production (Humphrey, 2006). Further, HS results in negative effects on growth performance and the relevant mechanisms were explored to mitigate the negative effects (Quinteirofilho et al., 2010;Quinteiro-Filho et al., 2012;Sandhu, Mirza, Afzal, & Mukhtar, 2012;Song et al., 2013). On the cellular level, high temperature promotes proteolysis and induces the production of excessive free radicals which caused oxidative stress damaging different organs of the body (Del Vesco et al., 2015), such as the liver and intestine. | MATERIALS AND METHODSThe study was conducted in a research farm (Hebei, China), and all procedures described here were approved by the Institutional Animal Care and Use Committee of the China Agricultural University (Beijing, China). | Animals and dietsA total of 720 four-day-old Peking ducks were randomly allotted to Table 1. And, the highest, the lowest and the comfortable temperature during study are shown in Table 2. In addition, mortality was recorded daily during the entire trial. And, mortality was almost no. | Sample collectionSix birds per treatment were randomly selected at 16 and 35 day age and euthanized by CO 2 inhalation and decapitation. Liver samples (~2 g) were collected and separated into two portions. Tissue samples were snap frozen immediately in liquid nitrogen and then stored at −80°C and −40°C until further qRT-PCR and protein carbonylation content analyses respectively. Tissue samples (~5-8 mm) were taken
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