Effects of nicotine on cultured cells suggest that it can influence the formation and resorption of bone

Yuhara, Sachiko; Kasagi, Satoshi; Inoue, Atsuto; Otsuka, Eri; Hirose, Shigehisa; Hagiwara, Hiromi

doi:10.1016/s0014-2999(99)00551-8

Cited by 91 publications

(80 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In a recent study our group demonstrated a significant increase in the TGF-β1 concentration in fracture haematoma and in serum of patients with long bone fracture. These results indicated the importance of this cytokine for fracture healing and confirmed other clinical and experimental studies [8][9][10][11][12][13][14][15][16][17]. Circulating TGF-β1 levels were found to be a predictor of delayed bone healing and non-union [11].…”

Section: Introductionsupporting

confidence: 86%

“…In an in vivo experiment, tobacco extract which did not contain nicotine significantly reduced the mechanical strength of healing femoral fractures in rats, while nicotine alone did not affect the mechanical properties [22]. Other studies have reported that nicotine stimulates osteoblastic proliferation, ALP activity, collagen and protein synthesis, and mineralization of the osteoblastic cells [16,23]. Higher levels of TGF-β1 in smoker's serum as found in our patients suggest the stimulating effect of nicotine on the osteoblasts.…”

Section: Discussionsupporting

confidence: 51%

“…Evidence exists that the local and systemic concentrations of different osteogenic growth factors are increased during fracture healing [8][9][10][11][12][13][14][15][16][17]. Among these factors TGF-β1 is known to be pivotal for the bone healing.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Is the expression of Transforming Growth Factor-Beta1 after fracture of long bones solely influenced by the healing process?

Kaiser

Thomas

Köttstorfer

et al. 2012

International Orthopaedics (SICOT)

View full text Add to dashboard Cite

Purpose Circulating TGF-β1 levels were found to be a predictor of delayed bone healing and non-union. We therefore aimed to investigate some factors that can influence the expression of TGF-β1. The correlation between the expression of TGF-β1 and the different socio-demographic parameters was analysed. Methods Fifty-one patients with long bone fractures were included in the study and divided into different groups according to their age, gender, cigarette smoking status, diabetes mellitus and regular alcohol intake. TGF-β1 levels were analysed in patient's serum and different groups were retrospectively compared. Results Significantly lower TFG-β1 serum concentrations were observed in non-smokers compared to smokers at week 8 after surgery. Significantly higher concentrations were found in male patients compared to females at week 24. Younger patients had significantly higher concentrations at week 24 after surgery compared to older patients. Concentrations were significantly higher in patients without diabetes compared to those with diabetes at six weeks after surgery. Patients with chronic alcohol abuse had significantly higher concentrations compared to those patients without chronic alcohol abuse. Conclusion TGF-β1 serum concentrations vary depending upon smoking status, age, gender, diabetes mellitus and chronic alcohol abuse at different times and therefore do not seem to be a reliable predictive marker as a single-pointin-time measurement for fracture healing.

show abstract

Section: Introductionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 51%

See 1 more Smart Citation

Is the expression of Transforming Growth Factor-Beta1 after fracture of long bones solely influenced by the healing process?

Kaiser

Thomas

Köttstorfer

et al. 2012

International Orthopaedics (SICOT)

View full text Add to dashboard Cite

show abstract

“…The level of alkaline phosphatase in blood is an indicator of bone formation 24 . In the present study, the low daily doses of nicotine did not cause significant alterations in the alkaline phosphatase levels.…”

Section: ■ Discussionmentioning

confidence: 99%

The effects of subcutaneous injection of nicotine on osseointegration of machined and anodized implants in rabbits

Linden

Bittencourt²,

Carli³

et al. 2018

Acta Cir. Bras.

View full text Add to dashboard Cite

Purpose: To evaluate the influence of subcutaneous injection nicotine in osseointegration process on different implant surfaces. Methods: Twenty-two male rabbits were distributed into two groups according to the subcutaneous injections: (1) nicotine 3 mg/day/kg and (2) 0.9 % NaCI 3 mL/day/kg, three times a day; subgroups were then designated-machined and anodized implants were placed in the right and left tibia bones, respectively. The animals were submitted euthanasia after periods of eight weeks to determine nicotine and cotinine levels, alkaline phosphatase and biomechanical analysis. Results: The plasmatic levels of nicotine and cotinine were 0.5 ± 0.28 ng/mL and 9.5 ± 6.51 ng/mL, respectively. The alkaline phosphatase analyses in blood levels in control group were observed 40.8 ± 11.88 UI/L and 40.75 ± 12.46 UI/L, for the surfaces machined and anodized, respectively. In the test group was observed levels 37.9 ± 4.84 UI/L, for both implant surfaces. No significant differences were observed between control and test groups and between the implant surfaces regarding alkaline phosphatase blood levels. For biomechanics, no significant differences were observed in control group between the machined (25±8.46 Ncm) or anodized (31.2 ± 6.76 Ncm) implants. However, the treatment with nicotine induced higher torque than control in both machined (38.3 ± 13.52 Ncm) and anodized (35.5 ± 14.17 Ncm) implants, with p = 0.0024 and p = 0.0121, respectively. Conclusion: Subcutaneous injection of nicotine following implant insertion didn't have effect on osseointegration, independently from the implant surface.

show abstract

“…São relatadas modificações citoplasmáticas, degeneração e inatividade dos fibroblastos [15][16][17] , além da diminuição do número destas células e da sua diferenciação 13,15,16,[18][19][20] . Estes efeitos foram também descritos nos osteoblastos, afetando a reconstituição após fraturas 21 . Consequentemente deficiências na síntese do colágeno são relatadas 15,17,18,22,23 .…”

unclassified

A influência da nicotina na densidade de colágeno em cicatrizes cutâneas, em ratos

Biondo-Simões

Tetilla

Biondo-Simões

et al. 2009

Rev. Col. Bras. Cir.

View full text Add to dashboard Cite

A influência da nicotina na densidade de colágeno em cicatrizes A influência da nicotina na densidade de colágeno em cicatrizes A influência da nicotina na densidade de colágeno em cicatrizes A influência da nicotina na densidade de colágeno em cicatrizes A influência da nicotina na densidade de colágeno em cicatrizes cutâneas, em ratos cutâneas, em ratos cutâneas, em ratos cutâneas, em ratos cutâneas, em ratosThe influence of nicotine on the density of collagen in cutaneous scars in rats The influence of nicotine on the density of collagen in cutaneous scars in rats The influence of nicotine on the density of collagen in cutaneous scars in rats The influence of nicotine on the density of collagen in cutaneous scars in rats The influence of nicotine on the density of collagen in cutaneous scars in rats Objetivo: Objetivo: Estudar a reação inflamatória e a deposição de colágeno na cicatrização de feridas cutâneas sob a influência da nicotina. Métodos: Métodos: Métodos: Métodos: Métodos: Analisaram-se as cicatrizes de feridas abdominais de ratos tratados com nicotina, 2 mg/kg/d, comparando-as às de ratos controle. O tratamento foi iniciado sete dias antes do ato operatório e mantido por sete ou 14 dias, no pós-operatório. Os cortes histológicos foram corados pela hematoxilina e eosina e neles por meio de escores estabelecidos, reconheceu-se a intensidade e o tipo da reação inflamatória. Cortes histológicos corados pelo Sirius Supra red F3BA permitiram reconhecer a densidade do colágeno. Resultados: Resultados: Resultados: Resultados: Resultados: Não houve diferença quanto à intensidade da reação inflamatória na análise de sete dias (p=0,165) e nem na de 14 dias (p=0,684). Pôde-se verificar que não existiu diferença significante na densidade de colágeno tipo I, na avaliação feita com sete dias (p=0,912) e com 14 dias (p=0,211). O grupo controle mostrava mais colágeno tipo III com sete dias (p=0,004), mas aos 14 não havia diferença significante (p=0,720). A quantificação do colágeno total, embora fosse maior no grupo controle, não o foi de forma significante em nenhum dos tempos estudados (p=0,103 aos sete e p=0,549 aos 14 dias). Conclusão:Conclusão: Conclusão: Conclusão: Conclusão: Não houve, nas cicatrizes dos animais tratados com nicotina, em relação aos controles, diferença quanto à intensidade do processo inflamatório, nem quanto à densidade do colágeno. . Vários estudos experimentais, realizados em diferentes condições, descreveram efeito inibitório da nicotina sobre a migração e diferenciação dos queratinócitos 11,12 , levando ao atraso da reepitelização 13 . Fang e Svoboda, estudando fibroblastos humanos, cultivados com diferentes concentrações de nicotina, observaram que existia inibição da diferenciação dos fibroblastos a miofibroblastos e atribuíram a isto a diminuição da contração das feridas 14 . São relatadas modificações citoplasmáticas, degeneração e inatividade dos fibroblastos [15][16][17] , além da diminuição do número destas células e da sua diferenciação 13,15,16,[18][19][20] . Estes efeitos for...

show abstract

Effects of nicotine on cultured cells suggest that it can influence the formation and resorption of bone

Cited by 91 publications

References 28 publications

Is the expression of Transforming Growth Factor-Beta1 after fracture of long bones solely influenced by the healing process?

Is the expression of Transforming Growth Factor-Beta1 after fracture of long bones solely influenced by the healing process?

The effects of subcutaneous injection of nicotine on osseointegration of machined and anodized implants in rabbits

A influência da nicotina na densidade de colágeno em cicatrizes cutâneas, em ratos

Contact Info

Product

Resources

About