Effects of different formulations on viability and medium-term storage of Metarhizium anisopliae conidia

Alves, R. T.; Bateman, Roy; Gunn, Jane; Prior, C.; Leather, Simon R.

doi:10.1590/s1519-566x2002000100013

Cited by 38 publications

(25 citation statements)

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“…3 [18] Matrix The diffractograms of the polymer nanocomposite and respective starting materials are shown in Figure 1. The same diffractograms profiles were obtained for all the matrix formulations (blank), and the formulation with the highest bentonite concentration (AB4) was chosen to illustrate.…”

Section: Fungal Suspensionsmentioning

confidence: 99%

Polymer/Layered Silicate Nanocomposite as Matrix for Bioinsecticide Formulation

Batista

Souza

Santos-Magalhães

et al. 2014

Macromolecular Symposia

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Summary: Due to current encouragement to the use of bioinsecticides for pest control and the susceptibility of biological agents to external factors, we investigated the use of a polymer nanocomposite (PLN, polymer/ layered silicate nanocomposite) as matrix to encapsulate an entomopathogenic fungus active against pest insects of palm trees. The beads were formed by extrusion and the following variables were assessed: fungus conidial concentration (series 1: 10 7 ; series 2: 10 8 and series 3: 10 9 conidia/mL) and nanolayered silicate concentration (0; 0.5; 1; 2 and 4%). The matrix was evaluated by X-ray powder diffraction and Fourier transform infrared spectroscopy and the following characteristics of the products were assessed: percent of encapsulated conidia, size distribution and polydispersity index, swelling index, formulation's in vitro ability to release conidia and stability under different storage temperatures. PLN, whose interactions could be visualized by FTIR, proved to be a potential matrix for this fungus, because, while composed by natural substances non-toxic to the environment, it succeeded to encapsulate high amounts of conidia (series 2). A barrier effect with bentonite increase was also demonstrated by increased fungus germination time and thermal stability.

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Section: Fungal Suspensionsmentioning

confidence: 99%

Polymer/Layered Silicate Nanocomposite as Matrix for Bioinsecticide Formulation

Batista

Souza

Santos-Magalhães

et al. 2014

Macromolecular Symposia

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“…Biocompatibility is typically tested explicitly with potential surfactants, but there are general guidelines that can narrow one's search. Aerial conidia are generally much more tolerant of surfactants than blastospores, submerged conidia, or hyphal formulations and many successful examples of their use are available in the literature (Daoust et al 1983;Alves et al 2002;Akbar et al 2005;Faria and Wraight 2007;Jin et al 2008). Blastospores, submerged conidia, and hyphae lack the hydrophobic properties of aerial conidia, which allows surfactants to interact directly with the outer membrane of the cell.…”

Section: Formulations Adjuvants Adherence and Interactionsmentioning

confidence: 99%

Ecological considerations in producing and formulating fungal entomopathogens for use in insect biocontrol

2009

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Insect pests persist in a wide-variety of agricultural, arboreal and urban environments. Effective control with fungal entomopathogens using inundation biocontrol requires an understanding of the ecology of the target insect, fungal pathogen, and the insect-pathogen interaction. Historically, the development of production and formulation processes for biocontrol fungi has primarily focused on reducing costs by maximizing the yield of infective propagules, increasing storage stability, and improving product form for ease of application. These goals are critical for commercialization but are often in conflict with environmental and ecological considerations. Critical parameters for selecting a fungal pathogen for use in inundation biocontrol include the cost-effective production of a stable, infective propagule that is suited for use in the environment where the insect must be controlled. Production processes can be manipulated nutritionally and environmentally to produce efficacious propagules or to direct fungal differentiation to propagule forms that may be better suited for use in specific environments. Formulation development must also consider ecological and environmental factors to maximize biocontrol efficacy. A basic understanding of the surface chemistries of the fungal propagule and insect, the interactions between a fungal propagule and the insect cuticle that lead to infection, and the impact of the environment on this interaction can aid in the development of effective formulations.

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“…However, they must be compatible with the microorganism used in the formulation. One of the first steps in the development of a mico-insecticide formulation is to evaluate the products' effect on the fungus, to select compatible components (ALVES et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Compatibility of Metarhizium anisopliae with liposoluble photoprotectants and protective effect evaluation against solar radiation

Mochi¹,

Monteiro²,

Pietro³

et al. 2017

Biosci. J.

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ABSTRACT:The aim of this study was to analyze the compatibility of the Metarhizium anisopliae JAB 68 isolate with liposoluble photoprotectants and the photoprotection conferred to its conidia. The fungus was cultivated in a medium with the photoprotectants Neo Heliopan ® BB, Neo Heliopan ® E 1000, Eusolex ® 6007 and Neo Heliopan ® AV in various concentrations. Vegetative growth, sporulation and germination were assessed to determine compatibility. Then, conidia were suspended in oil containing the compatible photoprotectants, in their respective concentrations, and exposed to 0-7 hours of light from a solar simulator operating at 1,000 W m -2 . Neo Heliopan BB ® and Neo Heliopan ® E 1000 were compatible with M. anisopliae at 2% and 1%, respectively. Eusolex ® 6007 and Neo Heliopan ® AV were compatible up to 8% a 7%, respectively. Conidia exposed to radiation from the solar simulator, with photoprotectant-free oil as its carrier, presented germination of 78.12% after five hours. When adding any photoprotectant to the conidia oily suspension, the germination percentage was always higher than that obtained in the control. Neo Heliopan ® BB at 1 and 2% and Neo Heliopan ® AV at 0.5% increased the germination. After 7 hours of exposure the germination percentages were 93.77, 94.55 and 98.82%, respectively. Eusolex ® 6007 was not as efficient as previous products, but in the same exposure period was able to protect the conidia (88.60% germination) when used at a 1% concentration. Adding photoprotectants in oily formulations of M. anisopliae protects against the harmful effects of UV radiation contributing to the fungus efficiency in the field.

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Effects of different formulations on viability and medium-term storage of Metarhizium anisopliae conidia

Cited by 38 publications

References 0 publications

Polymer/Layered Silicate Nanocomposite as Matrix for Bioinsecticide Formulation

Polymer/Layered Silicate Nanocomposite as Matrix for Bioinsecticide Formulation

Ecological considerations in producing and formulating fungal entomopathogens for use in insect biocontrol

Compatibility of Metarhizium anisopliae with liposoluble photoprotectants and protective effect evaluation against solar radiation

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