Effects of 2-phenoxyethanol Anaesthesia on Haematological Profile on Common Carp (Cyprinus carpio) and Rainbow Trout (Oncorhynchus mykiss)

Velíšek, Josef; Svobodová, Z.; Piačková, Veronika

doi:10.2754/avb200776030487

Cited by 17 publications

(16 citation statements)

References 11 publications

(6 reference statements)

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“…Rapid induction was coupled with longer recovery period in the present study. Previous studies on clove oil (Roubach et al 2005;Velisek et al 2005a, b;Hajek et al 2006), 2-phenoxyethanol (King et al 2005;Velisek et al 2007) showed increase in recovery period parallel to decrease in induction time.…”

Section: Discussionmentioning

confidence: 90%

Efficacy of clove solution on blood sampling and hematological study in Beluga, Huso huso (L.)

Hoseini

Ghelichpour

2011

Fish Physiol Biochem

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Two experiments were conducted to evaluate the efficacy of clove solution on anesthesia and hematological parameters in Beluga, Huso huso (L.). In the experiment 1, fish were exposed to concentrations of 300, 500, 700, 1,800 and 3,000 ppm clove solution and induction (stage 3 and 4) as well as recovery period lengths were recorded. These concentrations were led to induction and recovery time between 42-365 and 125-235 s, respectively, in dose-dependent manner. There was negative correlation between induction and recovery time (R(2) = 0.92). In the experiment 2, fish were exposed to each aforementioned concentrations and let to reach stage 4 anesthesia and then blood-sampled. Control group was sampled without anesthesia. Hematological studies between groups showed no significant changes in white blood cell (WBC) and differential leukocyte count (P > 0.05). Hematocrit (Hct), hemoglobin (Hb), red blood cell (RBC) and mean corpuscular hemoglobin concentration levels were significantly high in concentrations of 300-700 ppm compared to control, 1,800 and 3,000 ppm. Control and 500-3,000 ppm groups have similar mean corpuscular volume values and significantly higher than 300 ppm. Lower values of mean corpuscular hemoglobin were related to control, 3,000 and 1,800 ppm and higher values were observed in the others. Strong positive correlation were observed between induction periods and Hct, Hb and RBC levels (R(2) = 0.095, 0.99 and 0.92, respectively). Results suggested that anesthesia with low concentrations of clove solution over a long period is stressful compared to high concentrations over short period.

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Section: Discussionmentioning

confidence: 90%

Efficacy of clove solution on blood sampling and hematological study in Beluga, Huso huso (L.)

Hoseini

Ghelichpour

2011

Fish Physiol Biochem

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“…As fish differ in their response to the same anesthetic, use of different agents characterized with various concentrations seems to be necessary (Velíšek et al 2007a). Here, induction and recovery times upon applying foregoing concentrations are shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The efficacy and anesthetic effects of 2-PE have been proved for different fish species including silver carp (McCarter 1992), platy fish (Guo et al 1992), goldfish (Kaiser and Vine 1998), kutum (Javadi Moosavi et al 2014), rainbow trout (Velíšek and Svobodova 2004b), common carp (Velíšek and Svobodova 2004a;Velíšek et al 2007a) and great sturgeon ). Nevertheless, before using 2-PE for any fish in large scales, it would be better to calibrate it in a laboratory scale.…”

Section: Introductionmentioning

confidence: 99%

Analysis of primary and secondary stress responses in bighead carp (Hypophthalmichthys nobilis) by anesthetization with 2-phenoxyethanol

Akbary

Pirbeigi

Jahanbakhshi

2016

Int. J. Environ. Sci. Technol.

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The main objective of the present study was to shed light on the effects of 2-phenoxyethanol on possible primary (cortisol level) and secondary (hematological indices and glucose level) stress responses and changes in the activity of metabolic enzymes (AST, ALT and ALP) in bighead carp (Hypophthalmichthys nobilis). The fish were exposed to 0.1, 0.3, 0.5, 0.7 and 0.9 ml L -1 of 2-phenoxyethanol. 2-Phenoxyethanol induction and recovery times were 115-595 and 29.66-179.3 s, respectively. At a concentration of 0.1 ml L -1 , the anesthetic failed to induce deep anesthesia. The lowest effective concentration of 2-phenoxyethanol was determined to be 0.7 ml L -1 , whereas 0.9 ml L -1 was found to be the most effective one. Data showed that RBC, WBC, hemoglobin and hematocrit values were significantly high in some treatments compared to the control. MCV and MCH contents decreased significantly. MCHC, ALP, AST and ALT did not vary significantly. Plasma glucose and cortisol levels were significantly high compared to the control (in some doses). Moreover, Na ? , K ? , Ca 2? , Mg 2? and Cl -did not vary significantly. Findings suggest that blood parameters were affected by anesthetizing bighead with 2-phenoxyethanol at 0.9 ml L -1 to the lowest extent.

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“…No observable effect of the treatment on post-stripping mortality in the present study is consistent with earlier results of Mikolajczyk et al (2008) and Randák (2002). The used anaesthetic concentration of 0.30 ml·l -1 2-phenoxyethanol is considered safe for rainbow trout (Velíšek and Svobodová, 2004;Velíšek et al 2007), thus this anaesthetic may be assumed as safe of for grayling. Evidently, intramuscular injection had no effect on the post-stripping mortality of the females used.…”

Section: Discussionmentioning

confidence: 99%

The effect of selected ovulation-inducing preparations on post-stripping mortality and reproductive indicators of farmed European grayling (Thymallus thymallus L.)

et al. 2013

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An experiment on the effects of hormonal preparations (Gonazon™ and Supergestran containing gonadotropin-releasing hormone) on the course of reproduction of farmed grayling (Thymallus thymallus L.) broodstock and their post-stripping mortality was performed at the hatchery in spring 2009. Four-year-old marked grayling females (n = 80) were randomly divided into four groups. The fish were intramuscularly injected with Supergestran at a dose of 30 μg·kg -1 body weight (Group 1), with Gonazon TM at a dose of 30 μg·kg -1 (Group 2) and with saline 0.9% NaCl (Group 3), while fish of Group 4 were left without treatment (control group). No effect of treatment on the total number of ovulated females (70-80%) was found at the end of the stripping period. Slightly higher (nonsignificant) percentage of ovulated fish in the first stripping time (3 days post injection) was observed in fish treated with Gonazon. The 30-days post-ovulatory mortality remained unaffected by hormone treatments in all groups. Significant differences (P < 0.001) were found in the fertilization rate of egg samples from the first stripping time. The highest fertilization rate (98.6%) was found in fish treated with Supergestran, lower fertilization rates (61% and 65%) in fish treated with saline and control, respectively, and the lowest fertilization rate (39%) in fish treated with Gonazon. Other reproductive indicators remained unaffected by treatment in all groups. Based on our results, Supergestran is the most suitable preparation for the enhancing of artificial reproduction efficiency of farmed European grayling in fishery practice. The study brings important information about artificial reproduction of grayling broodstocks reared in controlled conditions.

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Effects of 2-phenoxyethanol Anaesthesia on Haematological Profile on Common Carp (Cyprinus carpio) and Rainbow Trout (Oncorhynchus mykiss)

Cited by 17 publications

References 11 publications

Efficacy of clove solution on blood sampling and hematological study in Beluga, Huso huso (L.)

Efficacy of clove solution on blood sampling and hematological study in Beluga, Huso huso (L.)

Analysis of primary and secondary stress responses in bighead carp (Hypophthalmichthys nobilis) by anesthetization with 2-phenoxyethanol

The effect of selected ovulation-inducing preparations on post-stripping mortality and reproductive indicators of farmed European grayling (Thymallus thymallus L.)

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