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Camellia sinensis Linn is a well-known medical herb that grows in various parts of the world. In the current study, the antimicrobial activity of methanol extract from C. sinensis leaves against Lactococcus garvieae and Aeromanas hydrophila isolated from rainbow trout (Oncorhynchus mykiss) was investigated. The growth inhibitory effects of green tea extract was determined by disc diffusion method (3 times on different days), minimum inhibitory concentration (MIC) using serial dilution and minimum bactericidal concentration (MBC). For the L. garvieae and A. hydrophila, the zone diameter inhibition (ZDI) of extract ranged 2.30 -16.5 mm. The highest ZDI (16.50 ± 1.12 mm) for L. garvieae was observed at 100 mg•ml −1 and for A. hydrophila (16.20 ± 0.95 mm) at 250 mg•ml −1 concentration of green tea extract (P < 0.05). At 20, 30, 40 and 100 mg•ml −1 concentrations of extract, ZDI values of A. hydrophila and L. garvieae showed significant difference (P < 0.05). The lowest MIC value for the extract was 0.8 mg•ml −1 against both L. garvieae and A. hydrophila. From the results of the present study, it can be concluded that methanol extract of C. sinenses leaves could be effective for the inhibition of A. hydrophila and L. garvieae in rainbow trout.

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How to cite this paper: Fereidouni, M.S., Akbary, P. and Soltanian, S. (2015) AbstractThis study was carried out to investigate the effect of different concentrations of garlic (Allium sativum L.) extract in fish diet on survival rate, digestive enzymes and some biochemical parameters of Mugil cephalus larvae. Three hundred and sixty fish with mean weight 0.45 g were randomly divided into equal four groups; each one contained 3 replicates. Fish was fed with diets containing 0% (control), 0.5%, 1% and 3% concentrations of garlic extract (GE) in diet. 30 days after feeding, survival rate, different biochemical (larvae extract total protein, albumin, globulin, glucose, cholesterol and triglyceride) parameters and digestive enzyme activity (lipase, protease and amylase) were evaluated. There was a significant increase in survival rate of all groups fed with GE as compared to the control diet (P < 0.05). The administration of A. sativum in all levels significantly decreased (P < 0.05) the content of cholesterol, triglyceride and glucose in larvae extract. Also total protein, albumin and globulin levels had significantly increased in all groups fed GE (P < 0.05). The highest total protein (2.13 ± 0.12 g/dL), albumin (0.37 ± 0 g/dL), globulin (1.76 ± 0.12 g/dL), amylase (9.25 ± 0.14 U/mg protein), protease (4.20 ± 0.08 U/mg protein) and lipase (2.62 ± 0.14 U/mg protein) and the lowest serum triglyceride (22.78 ± 0.20 mg/dL), glucose (8.76 ± 0.09 mg/dL), cholesterol (3.69 ± 0.07 mg/dL) levels were observed in fish fed 3% GE in diet. Garlic inclusion in fish diet at 3% concentration is therefore beneficial for use in aquaculture to improve the general health and digestive enzyme activity of M. cephalus larvae.

Serum biochemical parameters are important aspects in the management of endangered species. The values of these parameters can be used for confirming the maturity and for monitoring any changes in the quality of waters and related soils. The aim of this research was to determine the reference values of biochemical factors in Acantopagrus latus and Epinephelus coioides. Blood samples were collected from captured fish from coastal waters of Chabahar (Ramin waterfront). Serum levels of glucose, Blood Urea Nitrogen (BUN), cholesterol (CHO), triglyceride, total protein, albumin, calcium (Ca 2+ ), phosphorus (P), sodium (Na + ), bilirubin and potassium (K + ) were measured. The results showed that the levels of albumin and bilirubin in E. coioides were significantly higher than A. latus and there were significant differences in all of the serum values (except P and glucose) between A. latus and E. coioides (P < 0.05). It can be concluded that the values of blood biochemical parameters may be affected by physiological factors such as the species of fish.

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