Efficacy of clove solution on blood sampling and hematological study in Beluga, Huso huso (L.)

Abstract

Two experiments were conducted to evaluate the efficacy of clove solution on anesthesia and hematological parameters in Beluga, Huso huso (L.). In the experiment 1, fish were exposed to concentrations of 300, 500, 700, 1,800 and 3,000 ppm clove solution and induction (stage 3 and 4) as well as recovery period lengths were recorded. These concentrations were led to induction and recovery time between 42-365 and 125-235 s, respectively, in dose-dependent manner. There was negative correlation between induction and recovery time (R(2) = 0.92). In the experiment 2, fish were exposed to each aforementioned concentrations and let to reach stage 4 anesthesia and then blood-sampled. Control group was sampled without anesthesia. Hematological studies between groups showed no significant changes in white blood cell (WBC) and differential leukocyte count (P > 0.05). Hematocrit (Hct), hemoglobin (Hb), red blood cell (RBC) and mean corpuscular hemoglobin concentration levels were significantly high in concentrations of 300-700 ppm compared to control, 1,800 and 3,000 ppm. Control and 500-3,000 ppm groups have similar mean corpuscular volume values and significantly higher than 300 ppm. Lower values of mean corpuscular hemoglobin were related to control, 3,000 and 1,800 ppm and higher values were observed in the others. Strong positive correlation were observed between induction periods and Hct, Hb and RBC levels (R(2) = 0.095, 0.99 and 0.92, respectively). Results suggested that anesthesia with low concentrations of clove solution over a long period is stressful compared to high concentrations over short period.

Use of eugenol as an anaesthetic for Geophagus brasiliensis juveniles

“…For Pangasius hypophthalmus weighing 2 to 20 g, the minimum concentration of eugenol to induce anesthesia in less than three minutes was 53.8 to 81.5 mg L -1 and the maximum concentration that the fish recover at least five minutes was 65.9 to 105.8 mg L -1 (HOSEINI et al, 2013). The time for a fast anesthesia, loss of equilibrium and deep anesthesia is important for blood collection, handling and surgery (HOSEINI et al, 2010;HOSEINI and GHELICHPOUR 2011;HOSEINI and NODEH 2011;FABIANI et al, 2013). In this study, the concentration of 80 mg L -1 is the most appropriate, though having had no significant difference between doses of 50 and 100 mg L -1 , because the times for achieve the different stages were more homogeneous.…”

“…Due to the fact that each one requires different anesthetic concentration to induce the desired anesthesia stage, and this concentration may vary between species and sizes classes (HOSEINI et al, 2013). It is necessary to know the optimal concentration for each species of fish, because the use of wrong concentrations may cause unwanted effects, such as an increased stress and mortality of animals exposed to the drug (HOSEINI et al, 2010;HOSEINI and GHELICHPOUR, 2011;HOSEINI and NODEH, 2011).…”

Anaesthetic efficacy and biochemical effects of 1,8-cineole in rainbow trout (Oncorhynchus mykiss , Walbaum, 1792)

“…Recently, other synthetic compounds such as menthol (Fac ßanha & Gomes, 2005;Kasai, Hososhima & Yun-Fei, 2014;Mazandarani & Hoseini, 2017;Saydmohammed & Pal, 2009;da Silva, Oliveira & Nero, 2016), myrcene (Taheri Mirghaed, Ghelichpour & Hoseini, 2016), linalool Taheri Mirghaed et al, 2016) and 1,8-Cineole (cineole) (Mazandarani & Hoseini, 2017) have been used for anaesthesia in different species (Colossoma macropomum, Macrobrachium rosenbergii, Danio rerio, Oryzias latipes, Carassius auratus, Astyanax altiparanae, Cyprinus carpio and Rhamdia quelen). (80.8% cineole) has anaes- There are several reports suggesting that different anaesthetics and anaesthesia protocols affect fish biochemical parameters (Holloway, Keene, Noakes & Moccia, 2004;Hoseini & Ghelichpour, 2012;Hoseini, Hosseini & Nodeh, 2011;Hoseini & Nodeh, 2013; Vel ı sek, Stara, Li, Silovska & Turek, 2011;Vel ı sek, Stejskal, Kou ril & Svobodov a, 2009;Zahl, Kiessling, Samuelsen & Olsen, 2010). ; but is found in Cuminum cyminum, Rosmarinus officinalis, L. alba, Ocmium gratissimum and C. galioides (Echeverrigaray et al, 2003;Ribeiro et al, 2016;Saccol et al, 2013;Gachkar et al, 2007).…”

“…Cineole is the major component of essential oil in plants of genus Eucalyptus sp. Stress markers, enzymes' activity and haematological parameters have been found to change following anaesthesia in several studies (Holloway et al, 2004;Hoseini & Ghelichpour, 2012;Hoseini & Nodeh, 2013;Hoseini et al, 2011;Shaluei, Hedayati, Jahanbakhshi & Baghfalaki, 2012;Vel ı sek et al, 2009Vel ı sek et al, , 2011. This compound has antioxidant and anti-inflammatory effects [reviewed by Guimarães, Quintans and Quintans-J unior (2013)Cineole has antinociceptive property and blocks nerve excitability (Khalil et al, 2004;Liapi et al, 2008), and its anaesthetic efficacy has been studied and confirmed in common carp fingerlings (Mazandarani & Hoseini, 2017).…”

Influence of clove oil and eugenol on muscle contraction of silkworm (<i>Bombyx mori</i>)

“…Both clove oil and eugenol have been used to anesthetize rainbow trout (Oncorhynchus mykiss) (4), red pacu (Piaractus brachypomus) (5), beluga (Huso huso) (6), nile tilapia (Oreochromis niloticus) (7), and flowerhorn fish (Amphilophus labiatus × Amphilophus trimaculatus) (8). Furthermore, eugenol can be used in other aquatic animals such as Indian shrimp (Fenneropenaeus indicus) (9), whiteleg shrimp (Litopenaeus vannamei) (10), and the amphibians as African clawed frog (Xenopus laevis) (11), as well as mammals like rat and mouse (12,13).…”