Effect of Zwitterionic Polymers on Wound Healing

Abstract

The effect of a thin film of a zwitterionic random copolymer composed of carboxybetaine [1-carboxy-N,Ndimethyl-N-(2 -methacryloyloxyethyl)methanaminium inner salt] (CMB) and n-butyl methacrylate (BMA), poly(CMB-r-BMA) (CMB, 30 mol%), on the healing of a full-thickness excisional and incisional wound in hairless rats was examined. The poly(CMB-r-BMA) film significantly enhanced wound closure and complete healing of a full-thickness excisional wound compared with the effect of the poly(n-butyl methacrylate) (PBMA) and the poly(ethylene terephthalate) (PET) films. However, the poly(CMB-r-BMA) film did not enhance healing of a fullthickness incisional wound in hairless rats. The amount of proteins adsorbed and that of neutrophiles adhered onto the poly(CMB-r-BMA) film were significantly smaller than those onto the PBMA and PET films. The results suggested that various cells and growth factors in the wound exudate are utilized effectively by covering an excisional wound with the poly(CMB-r-BMA) film, resulting in acceleration of healing. In addition, the poly(CMB-r-BMA) film significantly enhanced healing of a full-thickness excisional wound in hairless rats compared with the effect of Tegaderm ® as wound dressings. The poly(CMB-r-BMA) film has potential as a new wound dressing.

Temperature-responsive polymer-brush constructed on a glass substrate by atom transfer radical polymerization

“…The non-specific adsorption of bovine serum albumin (BSA), human immunoglobulin G (IgG) and bovine plasma fibrinogen (BPF) to the glass substrate was evaluated using bicinchoninic acid (BCA) method (see Supporting information) [50].…”

Double network hydrogels from polyzwitterions: high mechanical strength and excellent anti-biofouling properties

“…Anti-biofouling materials, which resist protein adsorption or cell adhesion, have widespread demand for various biomedical applications such as contact lens 1 , endoscope 2 , artificial organs implants 3 , plasters for wound healing 4 , and biosensors. 5 Various hydrophilic anti-biofouling materials, such as polyethylene glycol (PEG) 6,7 , zwitterionic polymers of phosphobetaine (PB) 8 , sulfobetaine (SB) 9 , and carboxybetaine (CB) 10 have been presented.…”

Preparation of anti-fouling silicone elastomers by covalent immobilization of carboxybetaine

“…Subsequently, the films were sonicated for 120 min in PBS to detach any protein adsorbed onto the native PDMS and b-PDMS surfaces. Then, the solution was collected, and the amount of protein was determined by the Micro BCA (bicinchoninic acid) Protocol (Micro BCA Protein Assay Kit, Sangon Biotech Co., Ltd., Shanghai, China) (26)(27). Afterwards, the amount of adsorbed protein was calculated according to the standard protein curve, which was measured with different concentrations of a protein standard.…”