Effect of vitamin E supplementation of sheep and goats fed diets supplemented with polyunsaturated fatty acids and low in Se

Abstract

Effect of vitamin E supplementation of sheep and goats fed diets supplemented with polyunsaturated fatty acids and low in Se There are situations of low supply of both micronutrients. As VitE is involved in ruminal biohydrogenation of polyunsaturated fatty acids (PUFA) and their protection against oxidation in metabolism, diets supplemented with PUFA may challenge VitE to an extent making recommended supplies insufficient. Twelve goats and sheep each were fed a diet supplemented with PUFA and characterised by low Se and limited VitE contents during the last 2 months of gestation and the first 2 months of lactation. The basal diet consisted of hay and concentrate. Six goats and sheep received extra VitE, while the control groups received no extra VitE. Blood and milk samples were taken. In addition, liver, heart muscle and spleen samples were obtained from the offspring after slaughtering at an age of 8 weeks. No significant changes were observed in serum Se and VitE. A significant increase in serum VitE concentrations between 2 and 4 weeks postpartum (pp) was evident in the supplemented kids. In 4, 6 and 8 weeks pp, the serum concentrations of VitE in the supplemented kids were significantly higher compared to the unsupplemented group. In the kids, VitE was higher in liver of the supplemented groups. There were no significant differences in response to extra VitE between sheep and goat. The kids responded to serum VitE different from that of lambs, as a significant difference was observed between supplemented and unsupplemented animals in the goat kids, but not the lambs. In conclusion, goats and sheep have to be viewed differently and may not be considered alike relating to VitE/Se metabolism and requirements, especially in young animals. received no extra VitE. Blood and milk samples were taken. In addition, liver, heart muscle and spleen samples were obtained from the offspring after slaughtering at an age of eight weeks. No significant changes were observed in serum Se and VitE. A significant increase in serum VitE concentrations between two and four weeks pp was evident in the supplemented kids. Four, six and eight weeks pp, the serum concentrations of VitE in the supplemented kids were significantly higher compared to the unsupplemented group. In the kids, VitE was higher in livers of the supplemented groups. There were no significant differences in the response to extra VitE between sheep and goat. The kids responded in serum VitE different from the lambs, since a significant difference was observed in between supplemented and unsupplemented animals in the goat kids, but not the lambs. In conclusion, goats and sheep have to be looked at differently and may not be considered alike relating to VitE/Selen metabolism and requirements, especially in young animals. Effect of vitamin E supplementation2

A Summary of New Findings on the Biological Effects of Selenium in Selected Animal Species—A Critical Review

“…The fluorometric method has been succesfully employed to analyse selenium in feed [ 280 , 281 ], excrements, blood and tissues [ 281 ]. The FIA-GF-AAS (flow injection analysis-graphite furnace-atomic absorption spectrometry) system coupled with a hydride generation has been utilised to detect selenium in plasma and spleen samples [ 282 ]. VG-ICP-MS (vapor generation-inductively coupled plasma-mass spectrometry) has also been used as an analytical tool to analyse selenium content in feed, milk and whole blood [ 275 ].…”

Perfil proteico e metabolismo oxidativo de cordeiros experimentalmente infectados pelo Haemonchus contortus e suplementados com selênio e vitamina E

“…Estudos demonstraram que o uso concomitante da vitamina E e do selênio resultou em melhora da resposta imune de pequenos ruminantes quando comparados à suplementação de apenas um dos elementos (FINCH & TURNER, 1996;MILAD et al, 2001;LIESEGANG et al, 2008 pelo H. contortus não foi suficiente para estimular a produção dessas proteínas. Avaliando o perfil oxidativo dos animais, pôde-se observar que os valores de TBARS e GSHPx curiosamente se comportaram de forma semelhante, ou seja, quanto maior foi a produção de TBARS, maior foi os valores de GSH-Px (correlação positiva alta: r=1).…”

Effect of milking frequency and α-tocopherol plus selenium supplementation on sheep milk lipid composition and oxidative stability

“…The AOX capacity of vitamin E (administered alone or in combination with Se) has been evaluated in dairy cattle and goats, examining the effects on milk production, composition, and oxidation (Focant et al, 1998;Tufarelli and Laudadio, 2011). Other studies have investigated lamb AOX status when dams were supplemented with vitamin E (Capper et al, 2005;Liesegang et al, 2008). As stated by Politis (2012), most of these studies provide evidence that vitamin E supplementation reduces milk oxidation, although opposite results have been also reported (Slots et al, 2007); hence, further investigation is warranted.…”