Effect of Tyrosine Kinase Inhibitors on Tyrosine Phosphorylation and Motility Parameters in Human Sperm

Bajpai, Malini; Asin, Susana; Doncel, Gustavo F.

doi:10.1080/01485010390196715

Cited by 29 publications

(15 citation statements)

References 42 publications

(39 reference statements)

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“…It is well known that genistein is a tyrosine kinase inhibitor with an effect on sperm motility in a dose-dependent manner. Hence, Kumi-Diaka et al [46] reported that low doses of genistein did not influence mice sperm motility, but high concentrations of genistein interfered with percentage sperm motility, and it was confirmed in human spermatozoa [19,20], thereby showing that incubation of sperm with 400 µmol L -1 genistein decreases the sperm motility. In our study, we detected slight increase in motility (progressive and total) when genistein (10 µmol L -1 ) was present in the thawing media; however, there was a tendency to modify the pattern of movement (VCL, VAP, STR, BCF) when 1 µmol L -1 of genistein was added.…”

Section: Resultsmentioning

confidence: 94%

“…On the other hand, sperm protein tyrosine phosphorylation has been associated with capacitation, motility changes and hyperactivation. The inhibition of tyrosine kinase produces a decrease in the magnitude of motion parameters [19]. It is well known that genistein is a tyrosine kinase inhibitor with an effect on sperm motility in a dose-dependent manner.…”

Section: Resultsmentioning

confidence: 99%

“…In our study, we detected slight increase in motility (progressive and total) when genistein (10 µmol L -1 ) was present in the thawing media; however, there was a tendency to modify the pattern of movement (VCL, VAP, STR, BCF) when 1 µmol L -1 of genistein was added. This fact could be related to the inhibition of tyrosine kinase and capacitation process [19]. In the future, it is necessary to evaluate the different concentrations of genistein (in the range between 1 µmol L −1 and 10 µmol L −1 ) supplemented in the freezing and thawing medium, because it is important to know the concentration in which the decreased motility parameters are observed.…”

Section: Resultsmentioning

confidence: 99%

“…The possibility to modulate the capacitation process using exogenous molecules could increase the time of application of the cryopreserved spermatozoa with success. However, there is a lack of information about the capacity to modulate the capacitation and induce acrosome reaction, and the possible secondary damage of the use of these compounds on sperm functionality [19,20].…”

Section: Introductionmentioning

confidence: 99%

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Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

Martínez-Soto¹,

DiosHourcade²,

Gutiérrez‐Adán³

et al. 2010

Asian J Androl

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In this study, we evaluated the effects of genistein supplementation of the thawing extender on frozen-thawed human semen parameters. We analyzed the effect of supplementation on sperm motility, capacitation (membrane lipid disorder), reactive oxygen species (ROS) generation, chromatin condensation and DNA damage. Using this preliminary information, it maybe possible to improve the cryopreservation process and reduce the cellular damage. We have confirmed that the isoflavone genistein (10 mmol L -1 ) has antioxidant properties on the frozen-thawed spermatozoa. This results in a decreased ROS production that shows a slight improvement in the sperm motility, and decreases the membrane lipid disorder and DNA damage caused by cryopreservation. These results suggest an effect of genistein on sperm functionality that could be of interest for assisted reproduction treatments using frozenthawed human spermatozoa, but further studies will be necessary to confirm our findings and to evaluate the possible clinical applications.

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Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

Martínez-Soto¹,

DiosHourcade²,

Gutiérrez‐Adán³

et al. 2010

Asian J Androl

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“…Pada saat kapasitas dan motilitas spermatozoa, aktivitas protein tirosin kinase dapat dihambat dengan memberikan genistein (Bajpai, Asin, & Doncel, 2003). Shibayama et al, (2001) menyatakan bahwa genistein dengan dosis 10 µg, 100 µg dan 1000 µg yang diberikan pada tikus mampu menurunkan tingkat reseptor estrogen didalam sel Leydig, menurunkan motilitas spermatozoa serta menurunkan berat testis.…”

Section: Hasil Dan Pembahasanunclassified

Determination of Isoflavon Aglicone in Extract of Soymilk and Tempeh

2017

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AbstrakSusu kedelai dan tempe adalah pangan olahan yang digemari masyarakat Indonesia dan menjadi salah satu sumber protein nabati yang terbuat dari kedelai. Kedelai mengandung senyawa isoflavon glikosida yang bermanfaat untuk kesehatan sebagai pencegah penyakit kardiovaskuler, kanker, diabetes, hipertensi, osteoporosis, obesitas dan menopause. Isoflavon glikosida memiliki struktur yang lebih kompleks, sehingga perlu mengalami proses biokimiawi untuk menyederhanakan strukturnya agar mudah diserap oleh tubuh. Isoflavon glikosida akan disederhanakan menjadi bentuk aglikon selama proses fermentasi dan hidrolisis kimiawi. Genistein merupakan salah satu bentuk isoflavon aglikon yang banyak ditemui pada produk kacang-kacangan. Susu kedelai dan tempe merupakan produk olahan dari kedelai, perbedaannya adalah tempe telah mengalami proses fermentasi, sehingga diyakini memiliki kadar isoflavon aglikon yang tinggi. Penelitian ini bertujuan untuk menentukan kadar genistein pada ekstrak susu kedelai dan tempe dengan menggunakan spektrofotometri UV-Vis. Sampel diekstraksi dengan menggunakan etil asetat, sehingga diperoleh ekstrak kental, selanjutnya diukur kadar genistein dengan metode spektrofotometri UVVis, menggunakan baku standar. Hasil penelitian diperoleh kadar genistein pada ekstrak susu kedelai dan tempe yaitu masing-masing 0.613 dan 5.519% (b/b). Hal ini menunjukkan bahwa kadar isoflavon aglikon lebih tinggi pada tempe dibanding susu kedelai. Kata kunci: genistein, isoflavon aglikon, susu kedelai, tempe Abstract Soymilk and tempeh are a processed food that is popular with Indonesian society and becomes one of vegetable

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NTP‐CERHR expert panel report on the reproductive and developmental toxicity of genistein

Rozman

Bhatia

Calafat

et al. 2006

Birth Defects Research Pt B

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This report is prepared according to the Guidelines for CERHR Panel Members established by NTP/NIEHS. The guidelines are available on the CERHR web site (http://cerhr.niehs.nih.gov/). The format for Expert Panel Reports includes synopses of studies reviewed, followed by an evaluation of the Strengths/Weaknesses and Utility (Adequacy) of the study for CERHR evaluation. Statements and conclusions made under Strengths/Weaknesses and Utility evaluations are those of the Expert Panel and are prepared according to the NTP/NIEHS guidelines. In addition, the Panel often makes comments or notes limitations in the synopses of the study. Bold, square brackets are used to enclose such statements. As discussed in the guidelines, square brackets are used to enclose key items of information not provided in a publication, limitations noted in the study, conclusions that dif fer from those of the authors, and conversions or analyses of data conducted by the Panel. NIH Public Access PREFACEThe National Toxicology Program (NTP) and the National Institute of Environmental Health Sciences (NIEHS) established the NTP Center for the Evaluation of Risks to Human Reproduction (CERHR) in June 1998. The purpose of the Center is to provide timely, unbiased, scientifically sound evaluations of human and experimental evidence for adverse effects on reproduction and development caused by agents to which humans may be exposed.Genistein was selected for expert panel evaluation because of public concern for the possible health effects of human exposures. Genistein is a phytoestrogen found in some legumes, especially soybeans. Phytoestrogens are non-steroidal, estrogenic compounds that occur naturally in many plants. In plants, nearly all genistein is bound to a sugar molecule and this genistein-sugar complex is called genistin. Genistein and genistin are found in many food products, especially soy-based foods such as tofu, soy milk, and soy infant formula, and in some over-the-counter dietary supplements.To obtain information about genistein for the CERHR evaluation, the PubMed (Medline) and Toxline databases were searched through February 2006 with genistein and its CAS RN (446-72-0), soy, soya, and relevant keywords. References were also identified from databases such as REPROTOX ® , HSDB, IRIS, and DART and from the bibliographies of reports being reviewed.This evaluation results from the effort of a 14-member panel of government and nongovernment scientists that culminated in a public expert panel meeting held March [15][16][17] 2006. This report is a product of the expert panel and is intended to (1) interpret the strength of scientific evidence that genistein is a reproductive or developmental toxicant based on data from in vitro, animal, or human studies, (2) assess the extent of human exposures to include the general public, occupational groups, and other sub-populations, (3) provide objective and scientifically thorough assessments of the scientific evidence that adverse reproductive/ developmental health effects may be associated wi...

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Effect of Tyrosine Kinase Inhibitors on Tyrosine Phosphorylation and Motility Parameters in Human Sperm

Cited by 29 publications

References 42 publications

Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

Determination of Isoflavon Aglicone in Extract of Soymilk and Tempeh

NTP‐CERHR expert panel report on the reproductive and developmental toxicity of genistein

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