ABSTRACKMeasurement of antioxidant activity of ethanol extract of Marunggai leaves (Moringa oleifera Lam) was conducted. The aim research to obtain antioxidant activity using FRAP (Ferric Reducing Antioxidant Power). Marunggai leaves (Moringa oleifera Lam.) ware extracted using maceration method by ethanol 96 %. Free radical activity absorbance was measured with a spectrophotometer at a wavelength of 720 nm and the total value of antioxidant activity was calculated based on the data absorbance. The calculations showed that Marunggai leaves (Moringa oleifera Lam.) have the antioxidant activity with the value 7,923 mg AAE/g extract.
AbstrakSusu kedelai dan tempe adalah pangan olahan yang digemari masyarakat Indonesia dan menjadi salah satu sumber protein nabati yang terbuat dari kedelai. Kedelai mengandung senyawa isoflavon glikosida yang bermanfaat untuk kesehatan sebagai pencegah penyakit kardiovaskuler, kanker, diabetes, hipertensi, osteoporosis, obesitas dan menopause. Isoflavon glikosida memiliki struktur yang lebih kompleks, sehingga perlu mengalami proses biokimiawi untuk menyederhanakan strukturnya agar mudah diserap oleh tubuh. Isoflavon glikosida akan disederhanakan menjadi bentuk aglikon selama proses fermentasi dan hidrolisis kimiawi. Genistein merupakan salah satu bentuk isoflavon aglikon yang banyak ditemui pada produk kacang-kacangan. Susu kedelai dan tempe merupakan produk olahan dari kedelai, perbedaannya adalah tempe telah mengalami proses fermentasi, sehingga diyakini memiliki kadar isoflavon aglikon yang tinggi. Penelitian ini bertujuan untuk menentukan kadar genistein pada ekstrak susu kedelai dan tempe dengan menggunakan spektrofotometri UV-Vis. Sampel diekstraksi dengan menggunakan etil asetat, sehingga diperoleh ekstrak kental, selanjutnya diukur kadar genistein dengan metode spektrofotometri UVVis, menggunakan baku standar. Hasil penelitian diperoleh kadar genistein pada ekstrak susu kedelai dan tempe yaitu masing-masing 0.613 dan 5.519% (b/b). Hal ini menunjukkan bahwa kadar isoflavon aglikon lebih tinggi pada tempe dibanding susu kedelai. Kata kunci: genistein, isoflavon aglikon, susu kedelai, tempe Abstract Soymilk and tempeh are a processed food that is popular with Indonesian society and becomes one of vegetable
Phenolic is a compound that having an aromatic ring with one or more hydroxyl groups. Phenolic compounds in foods can be grouped into simple phenols and folic acid. This study aimed to determine the total phenolic content of the leaf extract of nutmeg (Myristica fragrans Houtt). Leaves of nutmeg extracted using 70% ethanol, then the extract conducting qualitative analysis by reaction with FeCl3 as specific reagent. The result of this reaction is green solution, it means there are phenolic compound in the extract. The quantitative process of total phenolic was analyzed by UV-Vis spectrophotometry. A comparison used in this analysis is gallic acid; it is a standard reference in the determination of total phenolic content. Sample measured at a wavelength of 860.7 nm, which is the maximum wavelength of gallic acid. Total average content of phenolic is 183.56-mgGAE/g extract. The ethanol extract of nutmeg's leaves have high total phenolic content, which means it is potential as antioxidant.
Glucosamine (GlcN) has great application in the food, cosmetic, pharmaceutical, and biomaterial areas; it is typically produced by hydrolysis in the exoskeletons of marine animals, one of the types of mud crab (Scylla serrata Forskal). Mud crab shells contain chitin and chitosan compounds which can be further derivatives for the production of GlcN HCl. The purpose of this study is to isolate and determine the level of GlcN HCl by hydrolysis of chitin and chitosan from mud crab shell. GlcN HCl was characterized by Fourier Transform Infra-Red (FT-IR) spectrophotometry, and its quantity was analyzed by UV-Vis spectrophotometry. Both methods used GlcN HCl as a standard for comparison and calculation of match factor (MF). The results of this study showed that the samples contain GlcN HCl either chitin or chitosan. FT-IR spectrum of chitin sample appears at wave number 1033 cm-1 , 1532 cm-1 , 3415 cm-1 whereas for chitosan at 1033 cm-1 , 1532 cm-1 , 3356 cm-1. Both sample spectrums showed vibration corresponding to ether (-O-), ethyl (CH2) and amine group (NH). The MF value of sample chitin and chitosan is 933 and 974, respectively; it shows that the resulting spectra are identical with the standard. The GlcN HCl level of sample chitin and chitosan was 553 and 993 mg/g, respectively. It can be concluded that the waste of mud crab shell has high potential as a natural source of GlcN HCl.
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