Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

Martínez-Soto, Juan Carlos; DiosHourcade, Juan de; Gutiérrez‐Adán, Alfonso; Landeras, J.; Gadea, J.

doi:10.1038/aja.2009.92

Cited by 59 publications

(27 citation statements)

References 58 publications

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“…Those results confirm the antioxidative influence of genistein and its ability of protect DNA [29]. These authors conclude that their observations can be useful in preparing semen for IVF procedures [28]. Although in our study genistein did not significantly alter the level of mitochondrial superoxide anion, it cannot be excluded that those changes actually occurred.…”

Section: Discussionsupporting

confidence: 44%

“…However, other reports indicate that genistein seems to affect spermatozoa in a dose-depend manner, and probably the reaction differs between species. For example, in a study by Martinez-Soto et al [28], genistein was hypothesized to possess antioxidant properties and to be able to protect spermatozoa DNA, endangered by damage resulting from oxidative stress. Next, to check the changes in cell quality, these authors analyzed the influence of supplementation of medium normally used for cryopreservation of spermatozoa, modified by adding genistein at a final concentration of 10 µmol/L.…”

Section: Discussionmentioning

confidence: 99%

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17β-estradiol and xenoestrogens reveal synergistic effect on mitochondria of human sperm

Skibinska

Jendraszak²,

Borysiak³

et al. 2016

Ginekol Pol

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Objectives: The aim of the study was to investigate the influence of 17β-estradiol (main endogenous estrogen) and selected xenoestrogens (genistein, bisphenol-A), individually and in combination, on the mitochondrial function of human spermatozoa. In natural environment, human beings are exposed to multiple xenoestrogens, so their impact is combined with endogenous steroids. Material and methods:The effects of ligands on human spermatozoa were assessed regarding the following phenomena: spermatozoa vitality (propidium iodide staining), phosphatidylserine membrane translocation (staining with annexin V marked with fluorescein), mitochondrial membrane potential (using JC-1 fluorochrome), and production of superoxide anion in mitochondria (using MitoSOX RED dye).Results: Two-hour incubation of spermatozoa with 17β-estradiol , genistein, and bisphenol-A neither altered cell vitality nor stimulated phosphatidylserine membrane translocation. Incubation of spermatozoa with 17β-estradiol or bisphenol-A separately, as well as incubation with the three ligands simultaneously, resulted in altered mitochondrial membrane potential. Spermatozoa incubation with the three ligands significantly increased the mitochondrial superoxide anion level. Conclusions:It seems safe to conclude that human spermatozoa mitochondria are target cell structures for both, 17β-estradiol and xenoestrogens. The reaction to the 17β-estradiol and xenoestrogens mixture suggests a synergistic mechanism of action. Xenoestrogens may increase the sensitivity of spermatozoa to 17β-estradiol.

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Section: Discussionsupporting

confidence: 44%

Section: Discussionmentioning

confidence: 99%

17β-estradiol and xenoestrogens reveal synergistic effect on mitochondria of human sperm

Skibinska

Jendraszak²,

Borysiak³

et al. 2016

Ginekol Pol

View full text Add to dashboard Cite

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“…This includes, antioxidant enzymes acting at the extracellular level, such as catalase [Li et al 2010] and SOD [Forouzanfar et al 2013;Roca et al 2005]; non-enzymatic antioxidants counteracting ROS generation at cell membrane, such as vitamin E [Kalthur et al 2011]; and non-enzymatic antioxidants increasing intracellular antioxidant capacity, such as glutathione (GSH) [Gadea et al 2011] or genistein [Martinez-Soto et al 2010].…”

Section: Discussionmentioning

confidence: 99%

Addition of Tempol in semen cryopreservation medium improves the post-thaw sperm function

Bateni¹,

Azadi²,

Tavalaee³

et al. 2014

Systems Biology in Reproductive Medicine

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Despite extensive research carried out for optimization and commercialization of sperm cryopreservation media, percentage of motility and viability remain low following cryopreservation. These adverse effects have been partially ascribed to reactive oxygen species (ROS) production during cryopreservation. Therefore, we proposed that addition of a cell permeable antioxidant like Tempol, with superoxide dismutase (SOD) mimetic action, may overcome these effects in an optimized commercially available cryo-protective medium. Therefore, semen samples were cryopreserved in the presence or absence of Tempol. A concentration of 5 μM Tempol was defined as optimal since it significantly improved motility and viability post thawing and reduced DNA fragmented sperm. In addition, percentage of ROS positive sperm was reduced. These effects of Tempol can be attributed to cell permeability characteristic and ability to reduce superoxide production both at intra- and extra-cellular levels. Tempol may hold the potential for clinical applications.

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“…[10][11][12][13][14] A beneficial effect of the in vitro supplementation of different natural or chemically synthesized antioxidant compounds on human spermatozoa has been observed; however, their effect has also been shown to decrease at inadequate concentrations and, even worse, to behave as pro-oxidants. 11,12,15,16 Herein, we discuss the protective role of exogenous gangliosides against ROS-induced changes based on our studies of human spermatozoa, leukocytes and cell-free systems. Of particular interest are differences between the effects of various types of gangliosides on the lipid peroxidation level, superoxide anion production [17][18][19][20] and H 2 O 2 and cryopreservation-induced DNA damage of human spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

Gavella¹,

Lipovac²

2013

Asian J Androl

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This article summarizes the available evidence on the efficacy of gangliosides to reduce the degree of reactive oxygen species (ROS)-mediated damage. The antioxidative efficacy of exogenous gangliosides in protecting different cells encouraged us to examine their ability to protect human spermatozoa. Gangliosides are sialic acid-containing glycosphingolipids with strong amphiphilic character due to the bulky headgroup made of several sugar rings with sialic acid residues and the double-tailed hydrophobic lipid moiety. The amphiphilicity of gangliosides allows them to exist as micelles in aqueous media when they are present at a concentration above their critical micellar concentration. The protective effect of ganglioside micelles on spermatozoa is believed to stem from their ability to scavenge free radicals and prevent their damaging effects. In our study, we particularly focused our attention on the protective effect of ganglioside micelles on DNA in human spermatozoa exposed to cryopreservation. The results indicate that ganglioside micelles can modulate the hydrophobic properties of the sperm membrane to increase tolerance to DNA fragmentation, thus protecting the DNA from cryopreservation-induced damage. Further actions of ganglioside micelles, which were documented by biochemical and biophysical studies, included (i) the modulation of superoxide anion generation by increasing the diffusion barrier for membrane events responsible for signal translocation to the interior of the cell; (ii) the inhibition of iron-catalysed hydroxyl radical formation due to the iron chelation potential of gangliosides; and (iii) inhibition of hydrogen peroxide diffusion across the sperm membrane.

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Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

Cited by 59 publications

References 58 publications

17β-estradiol and xenoestrogens reveal synergistic effect on mitochondria of human sperm

17β-estradiol and xenoestrogens reveal synergistic effect on mitochondria of human sperm

Addition of Tempol in semen cryopreservation medium improves the post-thaw sperm function

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

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