Effect of Polygonatum odoratum extract on human breast cancer MDA-MB-231 cell proliferation and apoptosis

Abstract

Abstract. Traditional Chinese medicine (TCM) is important in the provision of anti-tumor drugs. Recently, studies have shown that certain types of TCM agents are able to control the growth of tumors, enhance the body's immune function and enhance the therapeutic effect of chemotherapeutic drugs. In women, breast carcinoma is the most common tumor type and the second most common cause of death from cancer. Polygonatum odoratum (P. odoratum) is commonly used in TCM. The aim of the present study was to investigate the effects of P. odoratum extract on the proliferation and apoptosis of MDA-MB-231 breast cancer cells. Cell proliferation was assessed using MTT and colony formation assays. In addition, propidium iodide (PI)/Annexin V-FITC staining was used to investigate the apoptosis of MDA-MB-231 cells following treatment with P. odoratum extract. The protein expression levels of B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (Bax) were also detected using western blot analysis, while a JC-1 staining assay was used to assess the mitochondrial membrane potential (ΔΨm). The results of the MTT assay showed that the proliferation and colony formation of MDA-MB-231 cells were inhibited following treatment with the extract. Furthermore, the PI/Annexin-V staining showed that the apoptosis of MDA-MB-231 cells was enhanced by the extract, in a concentration-dependent manner. The extract also lowered the ΔΨm of MDA-MB-231 cells, upregulated the expression of Bax and inhibited the expression of Bcl-2. In conclusion, these results showed that the P. odoratum extract inhibited the proliferation and induced apoptosis of breast cancer MDA-MB-231 cells.

Investigation of Total Phenolic Content of Tilia dasystyla and Polygonatum orientale Desf Extracts and Their Cytotoxic Effect on the Osteogenic Sarcoma (Saos-2) Cancer Cell Line

“…This component showed cytotoxic effect on murine fibrosarcoma L929 and melanoma A375 cancer cells by interrupting of a mitochondria-mediated ROS-p38-p53 pathway and this caused the growth inhibition of cancer cell lines (19,20). P. odoratum extracts showed growth inhibition properties on human breast cancer MDA-MB-231 cells and induced apoptosis (9).…”

“…The rhizome of Polygonatum is used as an herbal medicine in traditional medicine. The anti-depressant, anti-inflammatory, anticancer, anti-fever, antioxidant, and antimicrobial activities of the Polygonatum rhizome have been shown in many studies (9,10). Variety of compounds such as polysaccharides, saponins, phytohormones, glycosides, flavonoids, and alkaloids have been isolated from Polygonatum rhizome.…”

Qiyusanlong decoction suppresses lung cancer in mice via Wnt/β-catenin pathway

“…Astragalus membranaceus (Huangqi) has a wide range of immunopotentiating effects and is widely used as an adjuvant medicine during cancer therapy (19). Polygonatum odoratum (yuzu) extracts induce apoptosis of MDA-MB-231 breast cancer cells (20) and A549 human lung cancer cells (21). Extracts of scolopendra (tianlong) subspinipes mutilans induce cell cycle arrest and apoptosis in A375 human melanoma cells (22).…”

Ethanol extract from Cnidium monnieri (L.) Cusson induces cell cycle arrest and apoptosis via regulation of the p53‑independent pathway in HepG2 and Hep3B hepatocellular carcinoma cells

“…Chinese medicinal herbs have been used for the treatment of various diseases in China and Southeast Asia (23). Also, many studies have focused on the anticancer effects of phytochemicals which is contained in natural plant (3,(7)(8)(9)(10). In the present study, we examined whether ethanol extracts from C. monnieri (CME)-induced apoptotic and cell cycle arrest effects were occurred by p53-dependent or p53-independent mechanism in HepG2 (wild-type p53) and Hep3B (p53-null) hepatocellular carcinoma cells.…”

“…A previous study reported that bioactive compounds from natural plant extracts play a crucial role as potential therapeutic agents (6). Many plant extracts induce anticancer effects such as anti-proliferative and anti-metastatic effects and cell cycle arrest in various cancer cell lines (7)(8)(9). Moreover, the anticancer activity of natural plant extracts has been investigated in HepG2 and Hep3B hepatocellular carcinoma cells (3,10).…”