Qiyusanlong decoction suppresses lung cancer in mice via Wnt/β-catenin pathway

Abstract

Lung cancer is one of the most fatal cancers due to its high metastatic rate. Traditional Chinese medicine has been used in cancer patients for decades to improve quality of life and prolong survival time. The present study used a novel Qiyusanlong (QYSL) decoction composed of 10 kinds of Chinese medicine including astragalus membranaceus (Huangqi), polygonatumod oratum (yuzu), scolopendra (tianlong), pberetima (dilong), solanum nigrum (longkui), herbahedyotis (baihushecao), semen coicis (yiyiren), euphorbia helioscopia (zeqi), curcuma longa (eshu) and tendril-leaved fritillary bulb (chuanbei). The effects and function of the QYSL decoction remain to be elucidated. The present study established a mouse xenograft model using Lewis lung carcinoma cell injection and administered different doses of QYSL decoction to the mice. It was demonstrated that the chemotherapy drug Cisplatin (DDP) and QYSL decoction repressed lung tumor growth, and the inhibitory effect of DDP was more significant. Furthermore, QYSL decoction and DDP modulated the expression of regulatory proteins in the Wnt/β‑catenin pathway, including Wnt1, Wnt2, Wnt5a and glycogen synthase kinase 3β, detected by western blotting, and affected the signals of cluster of differentiation 44 variation 6 and Survivin in tumor tissues, examined via immunohistochemistry. The combination of QYSL decoction and DDP enhanced the inhibitory effect. These data demonstrated that the QYSL decoction repressed lung tumor development via the Wnt/β‑catenin pathway. The therapeutic effect of QYSL decoction alone was milder compared with DDP, however the combination of QYSL decoction and chemotherapy exhibited an increased the rapeutic effect compared with the treatments administered alone. These findings revealed the function of QYSL decoction as a lung cancer treatment and provided insight for a novel lung cancer therapy.

Qiyusanlong Formula Induces Autophagy in Non-Small-Cell Lung Cancer Cells and Xenografts through the mTOR Signaling Pathway

“…e QYSL formula has noticeable effects on the prevention and treatment of NSCLC [12,13]. Our previous studies showed that the anticancer properties of the QYSL formula may be associated with autophagy although the specific mechanism was not yet clear.…”

“…A549 cells were suspended to a concentration of 1 × 10 7 cells/mL, and approximately 2 × 10 6 cells per 200 μL DMEM were injected subcutaneously into the flanks of the nude mice. When the average tumor volume reached 50-100 mm 3 , the mice were randomly divided into four groups (n � 10): (a) model group in which mice received 0.1 mL/10 g physiological saline via intragastric administration daily for two weeks; (b) QYSL group in which mice received 80.48 g/kg QYSL via intragastric administration daily for two weeks, as described in a preliminary study of the effective dose [12]; (c) RAPA group in which mice received 2 mg/kg RAPA [19] via intraperitoneal injection daily for two weeks; and (d) combined group in which mice received 80.48 g/kg QYSL [12] via intragastric administration and 2 mg/kg RAPA [19] via intraperitoneal injection daily for two weeks. e tumor volumes were measured using an electronic digital caliper every two days.…”

“…e formula consists of ten Chinese medicinal herbs: Astragalus, Solanum nigrum, Gecko, Earthwormg, Euphorbia helioscopia, Hedyotis diffusa Willd, Curcuma zedoaria, Coix lacryma, Polygonatum, and Fritillaria cirrhosa [12]. e untargeted metabonomics method based on UPLC-QTOF/MS has been used to screen out 21 biomarkers that may contribute to the treatment of lung cancer with the QYSL formula; the biomarkers were found to be mainly involved in sphingolipid and glycerophospholipid metabolism and fatty acid degradation [13].…”

YYFZBJS ameliorates colorectal cancer progression in ApcMin/+ mice by remodeling gut microbiota and inhibiting regulatory T-cell generation

“…Recently, Semen Coicis, Herba Patriniae, and monkshood are found to have multiple pharmacological activities, including anti-cancer effect [ 28 – 30 ]. Notably, Yi-yi-ren and Bai-jiang-cao, the most abundant ingredients in the recipe, showed anti-proliferative efficacy in several human cancer cell lines, as well as a suppressive effect on the development of aberrant crypt force (ACF) in Azoxymethane (AOM) treated mice [ 31 ]. Our previous work demonstrated that some TCM inhibited the proliferation of CRC cells in vivo and in vitro [ 32 , 33 ].…”

A Network Pharmacology Exploring the Mechanism of a Chinese Medicine Pair for the Treatment of Gout

“…Combined with three databases, the related indicators of active ingredients in DH and SG were synthetically predicted. GeneCards database (Wang et al 2019) (http://www.genecards.org/) automatically integrates gene-centric data from 125 network sources, while BATMAN-TCM (http://bionet.ncpsb.org/batman-tcm) (Tong et al 2018) ranks potential drug-target combinations based on their similarities to known drug-target relations. STITCH database (http://stitch.embl.de/) (Tran et al 2017) integrates many experimental and manual evidence sources with data mining and interactive prediction.…”