Effect of Papaya Seed Extract (Carica papaya Linn.) on Glucose Transporter 4 (GLUT 4) Expression of Skeletal Muscle Tissue in Diabetic Mice Induced by High Fructose Diet

Wulansari, Devyani Diah; Basori, Achmad; Suhartati, Suhartati

doi:10.22146/tradmedj.27926

Cited by 4 publications

(7 citation statements)

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“…Papaya seed extract is rich in flavonoids and phenolic acid having important role as antioxidants and anti-hyperglycemia. Previous studies have shown that papaya seed extract can increase GLUT4 expression in hyperglycemic mice so that it can increase glucose uptake into insulin sensitive tissue [9]. The results of this study indicated that papaya extract at a dose of 200 and 300 mg/kg BW were able to improve the atrophy of skeletal muscle cells that occurs in hyperglycemia conditions.…”

Section: Resultsmentioning

confidence: 50%

“…Prolonged fructose induction can cause desensitization and downregulation of insulin receptors so that it can interfere Tabel 1. Body weight and fasting blood glucose level in the diabetic and the treatment rat group at the initial and final of study with intracellular GLUT4 translocation into the plasma membrane of skeletal muscle cells which subsequently causes a decrease in glucose uptake by insulin-sensitive tissues such as skeletal muscles, causing hyperglycemia conditions [9,14]. In this study, the results showed that in hyper-glycemic conditions, the area of skeletal muscle cells in rat gastrocnemius was smaller (Figure 3b) than those of normal rat (Figure 3a).…”

Section: Resultsmentioning

confidence: 99%

“…Papaya seed extract is thought to reduce the rate of glucose absorption in the intestine, increase the use of glucose in the peripheral, stimulate the ability of pancreatic β cells to produce insulin or improve pancreatic β cells [8]. Papaya seed extract has also been investigated to increase GLUT 4 expression in the skeletal muscle cells of diabetic rats, thereby increasing glucose uptake into cells [9]. With increased expression of GLUT 4 in muscle cells it is expected to prevent atrophy.…”

Section: Introductionmentioning

confidence: 99%

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Ameliorative Effect of Carica papaya Seed Extract on Diabetic Rat Model with Muscle Atrophy

Wulansari

Wulandari

Risthanti

et al. 2019

MPI

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This study aims to prove that papaya seed extract has the potential to improve muscle cell atrophy in diabetic conditions. Type 2 diabetes mellitus (DM) animal model were made with conditions similar to type 2 DM in humans i.e. with long-term (56 days) high sugar (fructose) consumption. Then, the next 14 days were treated with papaya seed extract at the dose of 100, 200, and 300 mg/kg BW orally. Body weight and blood glucose levels were monitored throughout the study period. At the end of the study, histopathological examination of gastrocnemius skeletal muscle tissue was carried out using hematoxylin-eosin staining and measuring myocyte cell area. Results showed that there is a correlation between blood glucose levels with the area of muscle cells which explains that the decrease in blood glucose levels is in line with the increase in the area of muscle fiber cells. In the diabetic group and the treatment group the dose of 100 mg/kg BW has a smaller area, whereas in the treatment group the dose of 200 and 300 mg/kg BW has an area close to the area of the muscle cell in the normal group. This is supported by the results of measurements of the area of muscle fiber cells observed through cross sections and measured at the end of this study. The treatment group at the dose of 200 and 300 mg/kg BW did not have significant difference (P>0.05) compared to the normal group. It can be concluded that papaya seed extract therapy at the dose of 200 and 300 mg/kg BW on diabetic rats can reduce the fasting blood glucose levels so that it can ameliorate cell atrophy in the diabetic conditions.

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Section: Resultsmentioning

confidence: 50%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ameliorative Effect of Carica papaya Seed Extract on Diabetic Rat Model with Muscle Atrophy

Wulansari

Wulandari

Risthanti

et al. 2019

MPI

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“…Rats were induced by administering 20% fructose solution orally at a dose of 1.86 grams/kg body weight since day 1 until day 60 days which is expected to induce insulin resistance (Wulansari 2017). Celery extract is also given orally on day 1 until day 60 in adjusted dose of treatment.…”

Section: Methodsmentioning

confidence: 99%

“…The tissue pieces then colored by soaking in lithium carbonate and rinsing with running water. because it is able to recognize several epitopes and cross reactivity can occur (Konrad et al, 2002;Wulansari, 2017). pieces are placed in 1% alcoholic acid for a few seconds before rinsing again with running water and soaked in eosin for 5 minutes.…”

Section: Calculation Of Homa-irmentioning

confidence: 99%

Effect of Celery Extract on Fructose Induced Insulin Resistance Rattus norvegicus

Sukarno

Mustika

Rejeki

2021

FMI

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Diabetes mellitus (DM) is one of the global health problems and in the top 4th ranks as the main cause of death in developing countries (IDF, 2015). The pathogenesis of type II DM involves abnormalities in insulin secretion and activity that leads to insulin resistance. This research aims to study the efficacy of celery (Apiumgraveolens) as a prevention of insulin resistance. In this study, the samples were 45 Wistar rats (Rattus norvegicus), male sex, aged 4-6 weeks, weight 150-175 grams,and had normal fasting blood glucose levels by tested before treatment. The experimental animals were divided into 5 groups, K1 was negative control group (insulin resistance by given 20% fructose 1,86 g/kg BW PO qDay);K2 was positive control group (those given 20% fructose 1,86 g/kg BW PO qDayand standard insulin resistance therapy metformin 500 mg/kg BW PO qDay); the K3 treatment group was given 20% fructose 1,86 g/kg BW PO qDay and celery extract 200 mg/kg BW PO qDay; the K4 treatment group was given fructose 20% 1,86 mg/kg BW PO qDay and celery extract 400 mg/kg BW PO qDay; and the K5 treatment group was given fructose 20% 1,86 mg/kg BW PO qDay and celery extract 600 mg/kg BW PO qDay. The treatment had been given every day for 60 days.Fasting blood glucose levels were measured using a Glucometer. Fasting blood insulin levels were measured using ELISA, HOMA-IR was calculated using a standardized formula, and GLUT4 protein expression was measured using immunohistochemistry. It the end of the intervention, there was a significant decreased in fasting blood glucose (FBG) in K4 group compared with K1 (p <0.05), insulin resistance in K1 was characterized by a higher HOMA-IR value compared to the therapy group, especially K4 and K5 (p <0.05). There was an increase in GLUT-4 expression on K4 and K5 compared with K1 (p <0.05). It can be concluded that celery extract has antihyperglycemia effect and furthermore it can prevent insulin resistance condition.

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GLUT4 as A Protein Target for T2DM Therapy with Natural Compounds

2023

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WHO reported 1.9 million death cases of diabetes patients in 2019. Diabetes is caused by damage in the pancreas which resulted in a lack of insulin or insulin resistance. Medication for T2DM mainly focuses on lowering blood glucose and treating affected organs. Current medications are still lacking, thus research is needed in finding novel medications to accommodate T2DM. This paper aims to present the current research on potential plant extract in increasing GLUT4 translocation in diabetes conditions. insulin resistance state affecting GLUT4 translocation which is important in affecting glucose uptake. Some research shows that plant extract proved to be potential in increasing the translocation of GLUT4 and helping lowering blood glucose levels.

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Effect of Papaya Seed Extract (Carica papaya Linn.) on Glucose Transporter 4 (GLUT 4) Expression of Skeletal Muscle Tissue in Diabetic Mice Induced by High Fructose Diet

Abstract: Ethnobotany surveys show that papaya seeds are widely used as herbs for the management of some diseases such as abdominal discomfort, pain, malaria, diabetes, obesity, and

Cited by 4 publications

References 2 publications

Ameliorative Effect of Carica papaya Seed Extract on Diabetic Rat Model with Muscle Atrophy

Ameliorative Effect of Carica papaya Seed Extract on Diabetic Rat Model with Muscle Atrophy

Effect of Celery Extract on Fructose Induced Insulin Resistance Rattus norvegicus

GLUT4 as A Protein Target for T2DM Therapy with Natural Compounds

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