Diversidade genética de populações naturais de pariparoba [Pothomorphe umbellata (L.) Miq.] por RAPD

Abstract

* jsvalle@unipar.br RESUMO:O objetivo desse trabalho foi analisar a estrutura genética de populações de Pothomorphe umbellata (L.) Miq. com base em polimorfismos moleculares do tipo RAPD. Foram analisadas quatro populações naturais do estado de São Paulo (Jacareí, Jundiaí, Piquete e Ubatuba) e uma população do Paraná (Adrianópolis). Foram identificados 25 locos polimórficos (96,15%). Elevados índices de diversidade genética foram observados dentro das populações (Hs = 0,2220). Verificou-se que 65,33% da variabilidade genética total encontra-se dentro das populações e 34,67% entre as populações; índices estes, obtidos a partir do cálculo da divergência genética (G ST = 0,3467). Os resultados sugerem que essas populações possuem níveis elevados de variabilidade genética, a qual pode ser fortemente impactada pela ação humana. . Twenty-five polymorphic loci (96.15%) were identified. There were high levels of genetic diversity within populations (Hs = 0.2220). Of the total genetic variability, 65.33% is within populations and 34.67% among populations (G ST = 0.3467). Results suggest that these populations have high levels of genetic variability, which can be strongly impacted by human action. Palavras

Analysis of genetic diversity of a native population of Myrcia lundiana Kiaersk. plants using ISSR markers

“…Owing to the importance of many medicinal plant species for popular use, or even for use in the pharmaceutical industry, many studies on the genetic diversity of native populations have been carried out. In studies with S. reticulata and P. umbellata, high genetic diversity was observed within native populations (Valle et al, 2013;Lima et al, 2015). Bandeira et al (2010) observed low genetic diversity between native populations of Jaborandi.…”

“…Knowledge on genetic variability and its organization in plant species, represent important steps for the exploitation, definition of genetic conservation strategies, and breeding programs (Setotaw et al, 2010). Owing to the importance of several medicinal species, either for popular use or for the pharmaceutical industry, several studies on genetic diversity in native populations have been carried out, such as those on Senna reticulata (Lima et al, 2015), Pilocarpus pennatifolius (Bandeira et al, 2010), Pothomorphe umbellata (Valle et al, 2013), and Annona crassiflora (Telles et al, 2003).…”

Genetic analysis of Apuleia leiocarpa as revealed by random amplified polymorphic DNA markers: prospects for population genetic studies

“…In Erythrina velutina, 106 bands were detected . Analysis of Pothomorphe umbellata was based on only 25 bands (Valle et al, 2013). Although no data from sufficiency analyses were available, those studies revealed high rates if polymorphism, at 87.9, 85.7, and 96.1%, respectively.…”