Analysis of genetic diversity of a native population of Myrcia lundiana Kiaersk. plants using ISSR markers

Abstract

Myrcia lundiana Kiaersk. is a tree of the family Myrtaceae found in tropical and subtropical areas of the southern hemisphere that produces essential oil. The aim of this study was to characterize the genetic diversity of M. lundiana plants from a native population of Parque Nacional de Itabaiana, using inter-simple sequence repeat molecular markers. Thirty-five primers were tested, 20 of which were polymorphic, resulting in 135 polymorphic and informative bands. Results of the cluster analysis, obtained using the unweighted pair group method with arithmetic mean, grouped plants into three clusters: Cluster I - MLU001, MLU002, MLU003, MLU004, MLU005, MLU006, MLU018, MLU019, MLU020, MLU021, MLU022; MLU008, MLU011, MLU012, MLU014, MLU015, MLU017, MLU026, and MLU028; Cluster II - MLU007, MLU009, MLU010, MLU013, and MLU016; and Cluster III - MLU023, MLU024, MLU025, and MLU027. Jaccard similarity coefficients for pair-wise comparisons of plants ranged between 0.15 and 0.87. MLU014 and MLU015 presented low genetic diversity, with a similarity index of 0.87. Conversely, MLU007 and MLU019 presented high diversity, with a similarity index of 0.15. According to the structure analysis, three distinct clusters were formed. Genetic diversity of M. lundiana plants was intermediate, and expansion of its genetic diversity is necessary. MLU026 and MLU028 are the most suitable for selection in breeding programs, since they clearly represent all of the diversity present in these plants. Moreover, these results provide important information on the existing genetic variability, highlighting the importance of Parque Nacional de Itabaiana for the conservation of this species.

Research Article Assessment of genetic diversity of a native population of Eplingiella fruticosa: a plant with therapeutic potential.

“…This is because the genetic content is little influenced by the environment and can provide useful information for the management of the species and the development of strategies for the conservation of genetic resources (Celestino et al, 2015;Baruah et al, 2017). Since it is an aromatic species with medicinal potential and found in areas of intense anthropic activity, it is necessary to obtain information on the genetic diversity of natural populations of E. fruticosa, which may guide subsequent stages of conservation and use of this species (Alves et al, 2016;Brito et al, 2016).…”

Research Article Variability of <i>Myrciaria</i> <i>dubia</i> genotypes (Myrtaceae) in native populations of Roraima state

“…Studies by Goulão and Oliveira (2001) describe that the use of ISSR markers are useful in the identification of cultivars of fruit species that usually present a long juvenile period due to high reproducibility, providing advantages over other methods. The ISSR markers were effective in determining the genetic variability of M. dubia, in the same way as in other studies applied to the family Myrtaceae, as described by Oliveira et al (2014) with Psidium guajava L. (guava), Alves et al (2016) with Myrcia ludiana (Sw.) and Cruz et al (2016) with Plinia cauliflora (Brazilian grape tree). This emphasizes that the use of molecular biology techniques in the evaluation of little studied species, such as M. dubia, allows the selection of genotypes with greater divergence for new studies of backcrossing of the species in a short period of time (Gois et al, 2014).…”

Genetic diversity of cambui trees (Myrciaria floribunda (West ex Willd) O. Berg) differentiated by the color of the fruit

“…), Cruz et al (2016) used 18 primers, which resulted in 462 polymorphic bands (99.65%). In the study of genetic diversity among 28 individuals of canela-de-tabuleiro (Myrcia lundiana Kiaersk) originated from the Itabaiana National Park, Alves et al (2016) tested 35 primers that generated 135 polymorphic bands (93.75%). Evaluating the genetic distance between accessions of guava and araçazeiros from the genus Psidium, Oliveira et al (2014) obtained 216 polymorphic bands with the use of 17 primers.…”