RESUMO O objetivo deste trabalho foi avaliar o estabelecimento e crescimento in vitro de plantas de grápia (Apuleia leiocarpa(

O objetivo deste trabalho foi avaliar o efeito de épocas do ano e de clones na produtividade das minicepas e no enraizamento de miniestacas de erva-mate (Ilex paraguariensis A. St. - Hil.) tratadas ou não com ácido indolbutírico (AIB). Com esta finalidade, nas quatro épocas do ano realizou-se a coleta de brotos das minicepas de quatro clones de erva-mate, sendo que em cada coleta foi avaliada a porcentagem de sobrevivência das minicepas e o número de miniestacas. Posteriormente, os brotos foram seccionados em miniestacas de gema única, as quais foram ou não tratadas com 2000 mg L-1 de AIB. Aos 30 e 60 dias foram avaliadas as porcentagens de sobrevivência, enraizamento e calogênese, número e comprimento das raízes. As minicepas dos quatro clones de erva-mate apresentaram alta sobrevivência, além de produção constante de miniestacas dos clones 06SM17, 06SM12 e 06SM15 durante as quatro épocas do ano. O tratamento com AIB não incrementou no enraizamento das miniestacas de erva-mate. O processo rizogênico das miniestacas variou conforme o genótipo e a sazonalidade. O clone 06SM15 apresentou a maior capacidade rizogênica nas quatro épocas do ano. As melhores porcentagens de enraizamento foram verificadas nas épocas do outono e/ou inverno para os quatro genótipos avaliados.

The objective of this study was to evaluate the morphophysiological quality of yerba mate plantlets (Ilex paraguariensis A. St.-Hil.) produced by mini-cutting at four different times of year and cultivated in different containers. For the rooting of mini-cuttings, shoots of four yerba mate clones were collected four times during the year (April, July and October 2014 and January, 2015), cut into single-budded mini-cuttings and planted in equal proportions of pine bark-based commercial substrate, vermiculite and coarse sand. After 60 days of cultivation in a wet chamber, the rooted mini-cuttings were transferred to different containers: a 100 cm³ rigid polyethylene tube container and 500, 1500 and 3000 cm 3 polyethylene bags containing pine bark-based commercial substrate and underground soil (2:1 v/v). A completely randomized design was used in a 4 x 4 factorial scheme, with five replications ranging from 5 to 20 mini-cuttings per experimental unit. The plantlets were evaluated for survival percentage, shoot height, stem diameter, shoot height/stem diameter ratio and number of leaves at 30, 60, 90 and 120 days of cultivation. At 120 days of cultivation, 10 plantlets from each treatment were randomly picked to evaluate shoot and root dry mass, Dickson quality index, shoot height, surface area, total volume and number of root tips. The best combinations of shoot height, length, surface area, total volume and number of root tips and Dickson quality index were found in plantlets produced in July, 2014, followed by plantlets produced in October,2014 and January, 2015. The polyethylene bags allowed the production of yerba mate plantlets with satisfactory quality of shoot and roots, regardless of the volume. Yerba mate plantlets can be produced by mini-cutting from July to January in polyethylene bags. Key words: Ilex paraguariensis. Plantlet production. Polyethylene bags. Season of the year. Tube containers. ResumoO objetivo deste estudo foi avaliar a qualidade morfofisiológica de mudas de erva-mate (Ilex paraguariensis A. St.-Hil.) produzidas por miniestaquia em quatro épocas do ano e cultivadas em diferentes recipientes. Para o enraizamento das miniestacas, brotações de quatro clones de erva-mate foram coletadas em quatro épocas do ano (Abril, Julho e Outubro de 2014 e Janeiro de 2015), seccionadas em miniestacas de gema única e cultivadas em iguais proporções de substrato comercial à base de casca

This study aimed to evaluate the rooting capability of apical and nodal mini-cuttings of ipe-roxo (Handroanthus

The competence of adult plants of mate (Ilex paraguariensis St. Hil.) to undergo adventitious rooting is essential to their vegetative rescue by cuttings. This study aimed to evaluate the adventitious rooting competence of adult mate plants, and then rescue these plants by making cuttings from epicormic shoots induced in different trunk positions. The percent survival and rooting of cuttings from epicormic shoots induced by girdling in seven adult plants were evaluated. The two stock plants whose cuttings had the highest rooting competence were further pruned and girdled to evaluate the effect of the position of epicormic shoots on their adventitious rooting competence. The potential success of rescuing of the same adult plants was also studied with cuttings of epicormic shoots induced by coppicing. The shoots were sectioned in single-bud cuttings, either treated with 3,000 mg L-1 indolebutyric acid or untreated, and then cultivated in a humid chamber for 60 days, and then the percent survival and rooting of cuttings was evaluated. In conclusion, we found that it is possible to rescue adult mate plants by cuttings from epicormic shoots induced by pruning, girdling, or coppicing. The adventitious rooting competence of cuttings differs among adult mate plants, and depends on the position of the epicormic shoots on the trunk from which epicormic shoots are collected.

No abstract

The objective of this work was to evaluate different solvents and times in the ultrasound-assisted extraction of polyphenols, in order to quantify the total phenolic and flavonoid contents in and the antioxidant activity of mate (Ilex paraguariensis) leaves. The experiment was carried out in a completely randomized design, in a 8x3 (solvent x time of extraction) factorial arrangement. The solvent extractors were distilled water and aqueous solutions of ethanol, acetone, and methanol, acidified or not with hydrochloric acid, and the time of ultrasound-assisted extractions were 15, 30, and 45 min. All extractions were finished with a mechanical agitation of 30 min. Samples treated with acidified hydroalcoholic solvents resulted in higher values of total phenolics and flavonoids and antioxidant activity. The extraction using acidified hydroethanol solvent was not affected by the time in the ultrasound. The contents of total phenolics and flavonoids were highly correlated with each other and with the antioxidant activity, especially when it was determined by the ferric-reducing antioxidant power method. Acidified hydroethanol solvent in ultrasound for 15 min is the most suitable extraction condition for the quantification of the contents of total phenolics and flavonoids in and the antioxidant activity of mate leaves.

The objective of this work was to define an early selection strategy to identify Cabralea canjerana (Meliaceae) clones with high multiplication rate. A mini-garden of 109 clones of canjerana seedlings was established in a completely randomized design, in an acclimatized greenhouse. From seedlings, the mini-stumps and mini-cuttings were obtained. Mini-cuttings were collected at five different times, and the number of mini-cuttings per mini-stump, rooting percentage, and number of rooted mini-cuttings were quantified. The number of rooted mini-cuttings per mini-stump was the only trait that showed high correlation with the others. Five groups of clones based on the number of rooted mini-cuttings per mini-stump were separated using k-means clustering, and the genetic gain from selection and Pearson correlation were estimated. The selection of the two best groups in each evaluation period resulted in high genetic gains from selection for all evaluated traits. Early selection for the number of rooted mini-cuttings discarded 65% of the evaluated clones, which increases experimental precision in evaluations of traits associated with plantlet growth and quality. Early selection for the number of rooted mini-cuttings per mini-stump at different times allows the identification of Cabralea canjerana clones with high multiplication rate.