The competence of adult plants of mate (Ilex paraguariensis St. Hil.) to undergo adventitious rooting is essential to their vegetative rescue by cuttings. This study aimed to evaluate the adventitious rooting competence of adult mate plants, and then rescue these plants by making cuttings from epicormic shoots induced in different trunk positions. The percent survival and rooting of cuttings from epicormic shoots induced by girdling in seven adult plants were evaluated. The two stock plants whose cuttings had the highest rooting competence were further pruned and girdled to evaluate the effect of the position of epicormic shoots on their adventitious rooting competence. The potential success of rescuing of the same adult plants was also studied with cuttings of epicormic shoots induced by coppicing. The shoots were sectioned in single-bud cuttings, either treated with 3,000 mg L-1 indolebutyric acid or untreated, and then cultivated in a humid chamber for 60 days, and then the percent survival and rooting of cuttings was evaluated. In conclusion, we found that it is possible to rescue adult mate plants by cuttings from epicormic shoots induced by pruning, girdling, or coppicing. The adventitious rooting competence of cuttings differs among adult mate plants, and depends on the position of the epicormic shoots on the trunk from which epicormic shoots are collected.
Resumo Entende-se a ciclagem como o movimento dos nutrientes entre os diversos compartimentos (atmosfera-plantaanimal-solo) do sistema de produção agropecuária. A associação do sistema integração lavoura-pecuária com plantio direto possibilita alta entrada de resíduo vegetal pelo uso de culturas e pastagens tanto gramíneas como leguminosas as quais permitem a disponibilização mais rápida e intensa ou lenta e gradual de nutrientes ao sistema, principalmente na camada superficial. Estudos mostram que 60% a 70% do nitrogênio encontrado na biomassa vegetal é reciclado e novamente absorvido pelas plantas no cultivo. A quantidade de ciclagem de nutrientes pelo animal é definida pela intensidade de pastejo. Em geral, quanto mais animais por unidade de área maior será a ciclagem de nutrientes, porém haverá menor fixação de nutrientes em produto de orígem animal por unidade de forragem ingerida, e ambas as situações são afetadas pela intensidade de pastejo que pode contribuir com o retorno de nutrientes pelas fezes por bovino de 500kg de peso vivo ao sistema, com valores estimados de 22
The objective of this work was to evaluate different solvents and times in the ultrasound-assisted extraction of polyphenols, in order to quantify the total phenolic and flavonoid contents in and the antioxidant activity of mate (Ilex paraguariensis) leaves. The experiment was carried out in a completely randomized design, in a 8x3 (solvent x time of extraction) factorial arrangement. The solvent extractors were distilled water and aqueous solutions of ethanol, acetone, and methanol, acidified or not with hydrochloric acid, and the time of ultrasound-assisted extractions were 15, 30, and 45 min. All extractions were finished with a mechanical agitation of 30 min. Samples treated with acidified hydroalcoholic solvents resulted in higher values of total phenolics and flavonoids and antioxidant activity. The extraction using acidified hydroethanol solvent was not affected by the time in the ultrasound. The contents of total phenolics and flavonoids were highly correlated with each other and with the antioxidant activity, especially when it was determined by the ferric-reducing antioxidant power method. Acidified hydroethanol solvent in ultrasound for 15 min is the most suitable extraction condition for the quantification of the contents of total phenolics and flavonoids in and the antioxidant activity of mate leaves.
Schinus terebinthifolius (Anacardiaceae), popularly known as red aroeira, occurs naturally in Argentina, Paraguay, Uruguay, and Brazil. It has medicinal properties and is one of the species most sought by avifauna, so it is useful in heterogeneous reforestation intended for the recomposition of degraded areas of permanent preservation. Information about chromosomes, from simple chromosome counting to detailed molecular cytogenetics, is relevant in systematic and evolutionary studies. This study aimed to perform chromosomal characterization of 22 accessions of S. terebinthifolius native to Rio Grande do Sul, aiming to verify the intraspecific variability in this species, besides the conservation and sustainable use of this species. For the determination of chromosome number, tissues of root tips were subjected to a cold pretreatment (± 4°C) for 18 h. Afterwards, the roots were fixed in Carnoy 3:1 (ethanol/acetic acid) for 24 h at room temperature and stored under alcohol (70%) under cooling. The slides were prepared by the squashing technique and stained with 2% acetic orcein. The chromosome number of S. terebinthifolius found in 22 accessions was 2n = 28, and these studied accessions do not exhibit intraspecific variability.
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