Differentiation of poplar and willow clones using RAPD fingerprints

Lin, David; Hubbes, M.; Zsuffa, L.

doi:10.1093/treephys/14.10.1097

Cited by 36 publications

(30 citation statements)

References 15 publications

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“…This is the first report of the application of microsatellite DNA markers for hybrid poplar (P. x canadensis) cultivar fingerprinting and identification. Also, with the exception of 'I-214' (Castiglione et al 1993;Lin et al 1994), this is the first report for the application of RAPD markers for differentiation of the P. x canadensis cultivars studied here. Also, the RAPD markers that we used were more informative than those previously used for poplar clone fingerprinting (Castiglione et al 1993;Lin et al 1994;Sigurdsson et al 1995).…”

Section: Discussionmentioning

confidence: 74%

“…However, this method of clonal identification is difficult, ambiguous, time consuming and subjective. From the late 1980s, allozyme and RAPD markers have been successfully used for identification of clones and determination of their interrelationships in a number of poplar species (Rajora 1988(Rajora , 1989aRajora and Zsuffa 1989;Rajora and Dancik 1992;Castiglione et al 1993;Lin et al 1994;Sigurdsson et al 1995).…”

Section: Introductionmentioning

confidence: 99%

“…However, all cultivars could not be uniquely fingerprinted from their allozyme genotypes at nine polymorphic loci. RAPD markers have also been used to characterize a small number of a different set of P. x canadensis cultivars (Castiglione et al 1993;Lin et al 1994) other than those examined in our previous (Rajora and Zsuffa 1989) or this study. Allozymes are limited by the amount of polymorphism and RAPD markers by their dominant nature and technical inconsistency.…”

Section: Introductionmentioning

confidence: 99%

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Microsatellite DNA and RAPD fingerprinting, identification and genetic relationships of hybrid poplar (Populus x canadensis) cultivars

2003

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Microsatellite DNA markers of ten SSR loci and 248 RAPD loci (resolved by 26 RAPD primers) were used for DNA fingerprinting and differentiation of 17 widely grown Populus x canadensis syn. Populus x euramericana (interspecific Populus deltoides x Populus nigra hybrids) cultivars ("Baden 431", "Blanc du Poitou", "Canada Blanc", "Dorskamp 925", "Eugenei", "Gelrica", "Grandis", "Heidemij", "I-55/56", "I-132/56", "I-214", "Jacometti", "Ostia", "Regenerata", "Robusta", "Steckby" and "Zurich 03/3"), and determination of their genetic interrelationships. Informativeness of microsatellite and RAPD markers was also evaluated in comparison with allozyme markers for clone/cultivar identification in P. x canadensis. High microsatellite DNA and RAPD genetic diversity was observed in the sampled cultivars. All of the 17 P. x canadensis cultivars could be differentiated by their multilocus genotypes at four SSR loci, and were heterozygous for their parental species-specific alleles at the PTR6 SSR locus. Except for "Canada Blanc" and "Ostia", which had identical RAPD patterns, all cultivars could also be differentiated by RAPD fingerprints produced by each of the two RAPD primers, OPA07 and OPB15. For microsatellites, the mean number of alleles, polymorphic information content, observed heterozygosity, observed number of genotypes and the number of cultivars with unique genotypes per locus was 5.2, 0.64, 0.67, 5.7 and 2.2, respectively. For RAPD markers, the number of haplotypes per locus, and the number of cultivars with unique RAPD profiles per locus were 1.06 and 0.72, respectively. Overall, microsatellite DNA markers were the most informative for DNA fingerprinting of P. x canadensis cultivars. On the per locus basis, microsatellites were about six-times more informative than RAPD markers and about nine-times more informative than allozyme markers. However, on the per primer basis, RAPD markers were more informative. The UPGMA cluster plots separated the 17 cultivars into two major groups based on their microsatellite genotypic similarities, and into three major groups based on their RAPD fragment similarities. Both the microsatellite and RAPD data suggest that the cultivars "Baden 431", "Heidemij", "Robusta" and "Steckby" are genetically closely related. The inter-cultivar genetic relationships from microsatellite DNA and RAPD markers were consistent with those observed from allozyme markers, and were in general agreement with their speculated origin. Microsatellite DNA and RAPD markers could be used for clone and cultivar identification, varietal control and registration, and stock handling in P. x canadensis.

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Section: Discussionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microsatellite DNA and RAPD fingerprinting, identification and genetic relationships of hybrid poplar (Populus x canadensis) cultivars

2003

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“…A great amount of useful information for genetic characterization and identification of poplars has accumulated, coming from the use of both allozyme polymorphism [28,30] or DNA markers: RAPDs [4,23,29,31] AFLPs, [1,6,8,15,40], microsatellites (SSRs) [3,6,10,14,30] and ISSRs [20].…”

Section: Introductionmentioning

confidence: 99%

Développement de marqueurs microsatellites pour l’identification et la certification des clones commerciaux de peuplier en Espagne

et al. 2008

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-In order to protect the legitimate interests of the poplar breeders, growers and industries, we have developed a reliable and affordable certification tool for commercial poplars in Spain. We have carried out a genetic description of the 28 commercial clones of the current Spanish National Catalogue using 10 microsatellite molecular markers (SSR). The results clearly show that in the Spanish National Catalogue there are labelling mistakes including synonymies, supported by a combined probability of identity (PI) of 1.18 × 10 −9 . We have also demonstrate that it is possible to identify all the different clones (genotypes) with only three microsatellites, that show a combined probability of identity of 2.4 × 10 −4 , and we have optimised the protocols to reduce the time and the economical costs of the analysis. This SSR-based tool could be incorporated in the current certification protocols, to avoid duplication of accessions and identification mistakes.commercial poplar clone / microsatellite / identification / Spanish national catalogue Résumé -Développement de marqueurs microsatellites pour l'identification et la certification des clones commerciaux de peuplier en Espagne. Dans le but de protéger les intérêts légitimes des cultivateurs, des sélectionneurs et des industriels de la populiculture, nous avons mis au point un outil de certification fiable et accessible pour les clones de peupliers commerciaux en Espagne. Nous avons fait une description génétique des 28 clones commerciaux de l'actuel catalogue national espagnol en utilisant 10 marqueurs moléculaires microsatellites (SSR). Les résultats obtenus indiquent clairement que ce catalogue contient des erreurs d'identification, dont des clones synonymes, soutenues par une probabilité combinée d'identité (PI) de 1.18 × 10 −9 . Nous avons également démontré qu'il est possible d'identifier tous les génotypes différents avec 3 microsatellites, qui montrent une probabilité combinée d'identité de 2.4 × 10 −4 , et nous avons optimisé les protocoles afin de réduire le temps et le coût de l'analyse. Cet outil fondé sur les SSR pourrait être intégré dans les protocoles actuels de certification afin d'éviter la duplication des matériaux et les erreurs d'identification.clone commercial de peuplier / microsatellite / identification / catalogue national espagnol

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“…Since the late 1980s, several molecular marker approaches have been successfully used in a number of poplar species for the fingerprinting and identification of clones and the determination of their interrelationship. Allozyme [10,12,27] and randomly amplified polymorphic DNA (RAPD) [5,17,31] analyses were initially used for this purpose because of their simplicity and relatively low cost. However, the small numbers of polymorphism present in allozyme and lack of reproducibility of RAPD limit the usefulness of these markers.…”

Section: Introductionmentioning

confidence: 99%

ISSR and AFLP identification and genetic relationships of Chinese elite accessions from the genusPopulus

Gao¹,

Zhang²,

Liwang³

et al. 2006

Ann. For. Sci.

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-Inter-simple sequence repeat polymorphism (ISSR) and amplified fragment length polymorphism (AFLP) analysis techniques were used in this study for the genetic fingerprinting and identification of 28 important Chinese poplar accessions. After fingerprinting, the genetic relationships among the accessions were determined. Each of three ISSR primers and four AFLP primer pairs produced fingerprint profiles that were unique to each of the accessions studied, and thus could be used solely for their identification. In general, the molecular data separated accessions from different poplar sections, and also distinguished between native and exotic accessions. In conclusion, both ISSR and AFLP could be applied to identify large numbers of poplar accessions, and could also be used to rapidly determine the genetic relationships among them. Furthermore, it is useful to conduct comparative studies with different marker systems when investigating the genetic relationships of poplar accessions.

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Differentiation of poplar and willow clones using RAPD fingerprints

Cited by 36 publications

References 15 publications

Microsatellite DNA and RAPD fingerprinting, identification and genetic relationships of hybrid poplar (Populus x canadensis) cultivars

Microsatellite DNA and RAPD fingerprinting, identification and genetic relationships of hybrid poplar (Populus x canadensis) cultivars

Développement de marqueurs microsatellites pour l’identification et la certification des clones commerciaux de peuplier en Espagne

ISSR and AFLP identification and genetic relationships of Chinese elite accessions from the genusPopulus

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