Microsatellite DNA and RAPD fingerprinting, identification and genetic relationships of hybrid poplar (Populus x canadensis) cultivars

Rajora, Om P.; Rahman, Muhammad Habibur

doi:10.1007/s00122-002-1082-2

Cited by 109 publications

(75 citation statements)

References 24 publications

(54 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A great amount of useful information for genetic characterization and identification of poplars has accumulated, coming from the use of both allozyme polymorphism [28,30] or DNA markers: RAPDs [4,23,29,31] AFLPs, [1,6,8,15,40], microsatellites (SSRs) [3,6,10,14,30] and ISSRs [20].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Développement de marqueurs microsatellites pour l’identification et la certification des clones commerciaux de peuplier en Espagne

et al. 2008

View full text Add to dashboard Cite

-In order to protect the legitimate interests of the poplar breeders, growers and industries, we have developed a reliable and affordable certification tool for commercial poplars in Spain. We have carried out a genetic description of the 28 commercial clones of the current Spanish National Catalogue using 10 microsatellite molecular markers (SSR). The results clearly show that in the Spanish National Catalogue there are labelling mistakes including synonymies, supported by a combined probability of identity (PI) of 1.18 × 10 −9 . We have also demonstrate that it is possible to identify all the different clones (genotypes) with only three microsatellites, that show a combined probability of identity of 2.4 × 10 −4 , and we have optimised the protocols to reduce the time and the economical costs of the analysis. This SSR-based tool could be incorporated in the current certification protocols, to avoid duplication of accessions and identification mistakes.commercial poplar clone / microsatellite / identification / Spanish national catalogue Résumé -Développement de marqueurs microsatellites pour l'identification et la certification des clones commerciaux de peuplier en Espagne. Dans le but de protéger les intérêts légitimes des cultivateurs, des sélectionneurs et des industriels de la populiculture, nous avons mis au point un outil de certification fiable et accessible pour les clones de peupliers commerciaux en Espagne. Nous avons fait une description génétique des 28 clones commerciaux de l'actuel catalogue national espagnol en utilisant 10 marqueurs moléculaires microsatellites (SSR). Les résultats obtenus indiquent clairement que ce catalogue contient des erreurs d'identification, dont des clones synonymes, soutenues par une probabilité combinée d'identité (PI) de 1.18 × 10 −9 . Nous avons également démontré qu'il est possible d'identifier tous les génotypes différents avec 3 microsatellites, qui montrent une probabilité combinée d'identité de 2.4 × 10 −4 , et nous avons optimisé les protocoles afin de réduire le temps et le coût de l'analyse. Cet outil fondé sur les SSR pourrait être intégré dans les protocoles actuels de certification afin d'éviter la duplication des matériaux et les erreurs d'identification.clone commercial de peuplier / microsatellite / identification / catalogue national espagnol

show abstract

Section: Introductionmentioning

confidence: 99%

“…As many of them have shown their usefulness on different poplar species or hybrids, it is advisable to use them for the molecular description of commercial poplars, most of which are interspecific hybrids [3,15,29,40].…”

Section: Introductionmentioning

confidence: 99%

Développement de marqueurs microsatellites pour l’identification et la certification des clones commerciaux de peuplier en Espagne

et al. 2008

View full text Add to dashboard Cite

show abstract

“…In Europe, poplar species frequently used in breeding programmes in order to produce hybrids are the European black poplar (Populus nigra L.) and the North American cottonwoods P. deltoides and P. trichocarpa. The unit of cultivation and breeding in poplars is a clone, and individual cultivars are normally represented by a single clone [73]. Poplar plantations usually represent one single cultivar or clone in order to minimise the variability in growth and wood quality within the plantation.…”

Section: Historical Context Of Poplar Cultivationmentioning

confidence: 99%

Natural hybridization between cultivated poplars and their wild relatives: evidence and consequences for native poplar populations

Broeck

Villar²,

Bockstaele

et al. 2005

Ann. For. Sci.

View full text Add to dashboard Cite

-It is recognized that introgressive hybridization and gene flow from domesticated species into their wild relatives can have a profound effect on the persistence and evolution of wild populations. Here, we review published literature and recent data concerning introgressive hybridization involving numerous species of the genus Populus. First, we briefly refer to some concepts and terminology before reviewing examples of natural and anthropogenic hybridization. Second, we examine whether natural genetic barriers could limit introgressive hybridization. Threat and possible consequences of anthropogenic hybridization are discussed in order to finally suggest conservation strategies for native poplar populations.

show abstract

“…Microsatellite DNA, or simple sequence repeat (SSR) markers, is an ideal tool for cultivar identification due to their hypervariability and reproducibility (Sefc et al 1998, Testolin et al 2000, Rajora and Rahman 2003, Manel et al 2005. Such markers are codominant and are useful for evaluating genetic diversity and genetic relationships among individuals and populations (Wunsch and Hormaza 2002).…”

Section: Introductionmentioning

confidence: 99%

“…For example, individual cultivars of poplar are normally represented by a single clone (Rajora and Rahman 2003), whereas a certain native cultivar of sugi in the Kyushu region sometimes comprises several different clones (Miyajima 1989). Nangouhi also consists of several different clones (Uchida et al 1993, Ieiri andMiyajima 2000).…”

Section: Introductionmentioning

confidence: 99%

Genetic relationships and origin of commercial clones of Nangouhi, a vegetatively propagated cultivar of hinoki cypress (Chamaecyparis obtusa)

et al. 2008

View full text Add to dashboard Cite

Nangouhi, a vegetatively propagated cultivar of hinoki cypress (Chamaecyparis obtusa), includes several vegetatively propagated clones with commercial uses. The genetic diversity, relationships, and origin of Nangouhi were evaluated using ten highly polymorphic microsatellite markers and compared with those of natural hinoki populations. In terms of their genetics, Nangouhi clones appeared to be more closely related to each other than to natural populations. Parentage analysis indicated that clone N14, which is commonly found in the grounds of old shrines and temples, is a parent of 11 of the other clones, of which N6 and N13 had genotypes identical to N14 at eight and seven loci, respectively. These clones could have been produced by crossing N14 and genetically related individuals. Assignment tests were used to determine the genetic origin of Nangouhi clones using 25 natural hinoki populations as a reference. The possible sources of most of the clones were the Hikosan population in Kyushu and the Besshiyama population in Shikoku; however, several clones could not be assigned to any natural population. Crosses between Nangouhi and genetically unrelated plus tree clones and recurrent selection from the possible source populations are recommended for future breeding of Nangouhi.

show abstract

Microsatellite DNA and RAPD fingerprinting, identification and genetic relationships of hybrid poplar (Populus x canadensis) cultivars

Cited by 109 publications

References 24 publications

Développement de marqueurs microsatellites pour l’identification et la certification des clones commerciaux de peuplier en Espagne

Développement de marqueurs microsatellites pour l’identification et la certification des clones commerciaux de peuplier en Espagne

Natural hybridization between cultivated poplars and their wild relatives: evidence and consequences for native poplar populations

Genetic relationships and origin of commercial clones of Nangouhi, a vegetatively propagated cultivar of hinoki cypress (Chamaecyparis obtusa)

Contact Info

Product

Resources

About