Diferenciação in vitro de células-tronco mesenquimais da medula óssea de cães em precursores osteogênicos

Lima, Silvia Amélia Ferreira; Wodewotzky, T. I.; Lima-Neto, J. F.; Beltrão-Braga, Patrícia Cristina Baleeiro; Landim-Alvarenga, F. C.

doi:10.1590/s0100-736x2012000500016

Cited by 7 publications

(9 citation statements)

References 33 publications

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“…Wistar rat MSCs were cultured in Dulbecco’s modified Eagle medium (high glucose; Gibco Laboratories, Grand Island, NY, USA) supplemented with 20% fetal bovine serum (Sigma-Aldrich, St Louis, MO, USA), 100 UI/ml penicillin, 100 μg/ml streptomycin solution (Gibco Laboratories) and 3 μg/ml amphotericin B (Gibco Laboratories) [ 22 , 23 ]. The experiments were performed according to the Brazilian College for Animal Experimentation (COBEA) guidelines and were approved by the University’s Ethics Committee in Experimental Animal Use (CEEA/FMB/UNESP, protocol number 811/2010).…”

Section: Methodsmentioning

confidence: 99%

A new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells

et al. 2014

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IntroductionThe optimization of an organic scaffold for specific types of applications and cells is vital to successful tissue engineering. In this study, we investigated the effects of a new fibrin sealant derived from snake venom as a scaffold for mesenchymal stem cells, to demonstrate the ability of cells to affect and detect the biological microenvironment.MethodsThe characterization of CD34, CD44 and CD90 expression on mesenchymal stem cells was performed by flow cytometry. In vitro growth and cell viability were evaluated by light and electron microscopy. Differentiation into osteogenic, adipogenic and chondrogenic lineages was induced.ResultsThe fibrin sealant did not affect cell adhesion, proliferation or differentiation and allowed the adherence and growth of mesenchymal stem cells on its surface. Hoechst 33342 and propidium iodide staining demonstrated the viability of mesenchymal stem cells in contact with the fibrin sealant and the ability of the biomaterial to maintain cell survival.ConclusionsThe new fibrin sealant is a three-dimensional scaffolding candidate that is capable of maintaining cell survival without interfering with differentiation, and might also be useful in drug delivery. Fibrin sealant has a low production cost, does not transmit infectious diseases from human blood and has properties of a suitable scaffold for stem cells because it permits the preparation of differentiated scaffolds that are suitable for every need.

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Section: Methodsmentioning

confidence: 99%

A new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells

et al. 2014

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“…However, it is still unclear whether this will result in a better outcome and hence whether it is the most recommended [28]. Dogs as experimental models of stem cell treatments have provided preclinical information relevant to treatments in both human medicine and veterinary medicine [29].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Therapeutic Potential of Canine Yolk Sac Cells in Dogs with Hips Dysplasia

Miglino

2019

BJSTR

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Currently, stem cell therapy has been a useful tool in regenerative medicine with high therapeutic potential due to the capacity for self-renewal and differentiation of these cells. In recent years, orthopedics has been seeking new methods of treatment to obtain the effect of repair of articular defects more effectively and without invasive procedures. Therefore, many studies involving cell therapy in order to improve the articular repair are being conducted. The aim of this study was to evaluate the therapy with yolk sac cells in dogs with hip dysplasia. For the evaluation of the treated animals, we rated the lameness score, muscular atrophy score, a questionnaire about quality of life, radiographic evaluation, synovial fluid analysis and blood count. Bilateral intra-articular applications of yolk sac cells were performed on days 0, 30 and 60, being held the last valuation on day 90. The results showed no significant statistical difference when observed the treated animals compared to the control group. However, observing the clinical improvement of these animals throughout the treatment was evident that cell therapy is beneficial to the animals.

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“…osteogenic, adipogenic and chondrogenic cell lines, as preconized by several authors (Lima et al 2012;Dominici et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Cochlear epithelial of dog fetuses: a new source of multipotent stem cells

et al. 2017

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Hearing loss caused by the damage of cochlea sensory cells or neurons is a common human disease, but also affects dogs and other animals. To test their progenitor nature as potential value for future therapies, we characterized cells derived from the cochlear epithelium in dog fetuses. In total, 8 fetuses of 35-40 days of gestation, derived from castration campaigns, were investigated. Cells were analysed by the MTT colorimetric assay and in regard to cell cycle, differentiation capacities, immunophenotypes and qPCR analysis. In culture, cells had a fibroblast-like morphology. Phenotypic immunocharacterization showed positive staining for mesenchymal stem cell and pluripotency markers and were negative for hematopoietic cell markers. Cells possessed differentiation capacity for the three main cell lineages: osteogenic, adipogenic and chondrogenic, altogether indicating their nature as mesenchymal stem cells. Thus, cells derived from fetal cochlear tissues indeed may provide valuable sources of progenitor cells for cell therapy of canine deafness and other diseases.

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Diferenciação in vitro de células-tronco mesenquimais da medula óssea de cães em precursores osteogênicos

Cited by 7 publications

References 33 publications

A new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells

A new fibrin sealant as a three-dimensional scaffold candidate for mesenchymal stem cells

Evaluation of the Therapeutic Potential of Canine Yolk Sac Cells in Dogs with Hips Dysplasia

Cochlear epithelial of dog fetuses: a new source of multipotent stem cells

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