Diagnosis of botulism types C and D in cattle by a monoclonal antibody‐based sandwich ELISA

Abstract

This study was undertaken to evaluate two monoclonal antibody-based sandwich ELISAs (sELISAs) for the detection of Clostridium botulinum neurotoxins (BoNTs) types C and D from culture-enriched intestinal content samples from cattle. To validate the diagnostic significance of the presence of cultivable, toxin-producing C botulinum in the intestines of cattle, samples from both suspect and non-suspect botulism cases were examined. BoNT was detected by both sELISAs in a greater number of suspect animals than by direct testing of uncultured samples by mouse bioassay. One sELISA detected two BoNT C and one BoNT Group III mosaic isoform in three animals that were missed by the other, and both sELISAs failed to identify samples from two mouse bioassay-positive BoNT C animals. BoNT D was also detected in one non-suspect sample by one of the sELISAs.

Detection, differentiation, and identification of botulinum neurotoxin serotypes C, CD, D, and DC by highly specific immunoassays and mass spectrometry

“…3B-E, left panel). [46][47][48]79,80 An exception was the most recent ELISA described as multiplex approach to detect six BoNT serotypes including BoNT/C and DC which reached sensitivities similar to those of the ELISAs established in this work (LODs: 2.2 and 0.9 pg mL −1 for BoNT/C and DC, respectively). 3B-E (right panel) displays standard curves of three experiments for each of the four toxins, including a four-parametric curve fit.…”

Microbiological Botulinum Toxins Removing From Drinking Water Sources by Treatment of Coagulation Process

“…In the present study, these results had showed some similarities observed as in under developing countries like rural area of Pakistan has not correct quality of drinking sources of water for domestic purposes. Same results derived as given by the study's results from Brooks et al (2011), it reported that in most of the developing countries, the quality of drinking water very not good as find some bacterial specious as per 100 ml of potable water used in domestic purpose. The recommend drinking water values of all limits under WHO guidelines is 100 ml per sample for detecting the total coliform group [15].…”

“…C. Boulinum with Types A, B, E, and only F in rare cases found which cause illness in humans and animals. Especially C. Bolulinum with Types C and D cause illness in mammals and birds and type G are identified in 1970 which cause of infection in humans or animals [2]. The word of Botulism read from the language Latin as botulus, and its meanings is sausage.…”

Accuracy of a Mouse Bioassay for the Diagnosis of Botulism in Horses

“…This observation parallels findings in other species. For example, researchers have observed a low occurrence of C. botulinum spores in intestinal samples from clinically normal cattle . In addition, isolation of C. botulinum from fecal or GI content samples from people is considered confirmatory evidence for botulism, because the organism is rarely encountered in specimens from humans in the absence of disease .…”