Development of ic-ELISAs for the Detection of Bisphenol A Diglycidyl Ether and Its Derivatives in Canned Luncheon Meats

Abstract

Canned foods are widely consumed because of their convenience, hygiene, and nutrition. Bisphenol A diglycidyl ether (BADGE), an industrial product of epichlorohydrin and bisphenol A, widely exists in the inner coating of canned foods. A hydrolysis or chlorination reaction of BADGE can occur in the process of storage, forming a variety of derivatives such as bisphenol A (2,3dihydroxypropyl) glycidyl ether (BADGE•H 2 O), bisphenol A (3-chloro-2-hydroxypropyl) glycidyl ether (BADGE•HCl), and bisphenol A (3-chloro-2-hydroxypropyl) (2,3-dihydroxypropyl) glycidyl ether (BADGE•HCl•H 2 O), which seriously threaten consumer's health. In this research, two haptens were successfully synthesized, and antibodies were obtained by the subcutaneous immunization of New Zealand rabbits. Indirect competitive enzyme-linked immunosorbent assays (ic-ELISAs) were established for the detection of BADGE, BADGE•H 2 O, BADGE•HCl, and BADGE•HCl•H 2 O, and the IC 15 values (concentrations at an inhibition rate of 15%) were determined as 0.73, 0.39, 0.78, and 1.45 ng/mL, respectively. The recovery of BADGE and its derivatives at various spiking levels in canned luncheon meat samples ranged from 76.70 to 98.35%. The results detected by the proposed ic-ELISAs were also validated by HPLC analysis, which showed a good agreement (R 2 = 0.9861).

Recent Advances in Sources, Migration, Public Health, and Surveillance of Bisphenol A and Its Structural Analogs in Canned Foods

“…In contrast, the GC technique implied that a derivatization process can simultaneously determine all studied compounds with sufficient repeatability and accuracy [42]. Moreover, an enzyme-linked immunosorbent assay (ELISA) technique was established for the determination of BPA and its analogs in several studies [43]. The main advantages of these detectors include their high selectivity, high sensitivity, and universality [42].…”