As a major metabolite of pyrethroid
pesticides, 3-phenoxybenzoic
acid (3-PBA) can be an indicator of health risk and human exposure
assessment. A gold–platinum (Au@Pt) nanozyme is a kind of mimic
enzyme with unique properties of nanomaterials and catalytic activity.
The anti-3-PBA nanobodies were labeled with Au@Pt (Au@Pt-Nbs) by electrostatic
adsorption. The Au@Pt-Nbs lateral-flow nanozyme immunoassay was established
for the visual and rapid detection of 3-PBA. The visualization results
were quantitatively analyzed by Adobe Photoshop CC and got a cutoff
value of 0.005 ng/mL. The samples of milk- and lake water-spiked 3-PBA
were also analyzed, and the recoveries ranged from 102 to 108% and
95 to 112%, respectively. The results were also validated by liquid
chromatography–mass spectrometry and got good correlation (R
2 = 0.945). The Au@Pt-Nbs lateral-flow nanozyme
immunoassay was developed by the combination of nanobodies and nanozymes,
which will be a promising application in the field of safety inspection.
Canned foods are widely consumed because of their convenience, hygiene, and nutrition. Bisphenol A diglycidyl ether (BADGE), an industrial product of epichlorohydrin and bisphenol A, widely exists in the inner coating of canned foods. A hydrolysis or chlorination reaction of BADGE can occur in the process of storage, forming a variety of derivatives such as bisphenol A (2,3dihydroxypropyl) glycidyl ether (BADGE•H 2 O), bisphenol A (3-chloro-2-hydroxypropyl) glycidyl ether (BADGE•HCl), and bisphenol A (3-chloro-2-hydroxypropyl) (2,3-dihydroxypropyl) glycidyl ether (BADGE•HCl•H 2 O), which seriously threaten consumer's health. In this research, two haptens were successfully synthesized, and antibodies were obtained by the subcutaneous immunization of New Zealand rabbits. Indirect competitive enzyme-linked immunosorbent assays (ic-ELISAs) were established for the detection of BADGE, BADGE•H 2 O, BADGE•HCl, and BADGE•HCl•H 2 O, and the IC 15 values (concentrations at an inhibition rate of 15%) were determined as 0.73, 0.39, 0.78, and 1.45 ng/mL, respectively. The recovery of BADGE and its derivatives at various spiking levels in canned luncheon meat samples ranged from 76.70 to 98.35%. The results detected by the proposed ic-ELISAs were also validated by HPLC analysis, which showed a good agreement (R 2 = 0.9861).
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