DEVELOPMENT OF DNA MARKERS (ISSRs) IN MANGO

González, Alberto; Coulson, M. A.; Brettell, R. I. S.

doi:10.17660/actahortic.2002.575.13

Cited by 47 publications

(31 citation statements)

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“…For being dominant markers, prior knowledge on the genome is not required (González et al, 2002). Moreover, ISSR markers have a high degree of polymorphism, high reproducibility, when compared with other markers, and can achieve results in a timely and cost-effectively manner (Borba et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

Almeida-Pereira¹,

Silva²,

Alves³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Brazil has about 300 Croton species in different types of vegetation. Croton tetradenius Baill., which is endemic to the Northeast region and predominant in the Caatinga vegetation, stands out among the several species of this genus. Considering the importance of knowing the genetic variability of a species, the objective of this study was to analyze the genetic diversity of the genotypes of natural populations of C. tetradenius in the State of Sergipe, using ISSR molecular markers. Forty individuals were sampled in four natural populations of the State of Sergipe, Brazil. Thirteen primers were used for DNA amplification using ISSR-PCR, totaling 77 amplified fragments, of which 94.8% were polymorphic. Results of the cluster analysis obtained by the Jaccard's similarity index, using the UPGMA method, resulted in the formation of six distinct clusters. Analysis of molecular variance (AMOVA), used to estimate the genetic variability among populations, revealed significant genetic variance (P < 0.01) between and within the 2 C.S. Almeida-Pereira et al.Genetics and Molecular Research 16 (2): gmr16029602 studied populations, and most of the genetic diversity was found (87%) within populations. According to the Jaccard's similarity index, none of the studied plants was genetically identical. CTE210 and CTE305 presented high similarity index (0.76), while CTE105 presented low similarity index (<0.16) with all related individuals. ISSR markers were efficient and allowed the formation of a molecular profile, and had sufficient polymorphism to estimate the genetic variability between the accessions of the studied populations.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

Almeida-Pereira¹,

Silva²,

Alves³

et al. 2017

Genet. Mol. Res.

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“…The DNA of the 102 plants was extracted according to [2], with modifications. Two hundred milligrams of leaves were macerated to powder together with liquid nitrogen, in a porcelain mortar.…”

Section: Methodsmentioning

confidence: 99%

“…They are highly polymorphic and reproducible, do not require previous knowledge about the genome and are relatively inexpensive [2]. They are also versatile and can be used in plant breeding programs, besides evolution studies, due to their ability to show differences among cultivars [3].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of ‘Ubá’ Mango Tree Using ISSR Markers

Rocha

Salomão

et al. 2011

Mol Biotechnol

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In this study, the genetic diversity of 'Ubá' mango trees cultivated at the Zona da Mata of Minas Gerais State, Brazil, was assessed, to identify whether there is variability in the plants grown in the region, justifying the mass selection as a breeding method. We used 102 accessions. Leaves were collected for extraction of genomic DNA, which was amplified with nine ISSR primers. The data obtained by the analysis of electrophoretic patterns were arranged in a binary matrix, considering 0 for the absence and 1 for the presence of bands. Based on these data, we performed the analysis of genetic dissimilarity and carried out the cluster analysis by the methods of Tocher and graphical dispersion. The most similar accessions are 144 and 150, both coming from Ubá, while the most divergent ones are 29 and 97, from Visconde do Rio Branco. The grouping by the Tocher method separated the accessions into six groups, 94.1% of which were allocated in the first group and showed that there is no separation of accessions depending on the sampling sites. The 3D scatter plot reinforces this conclusion. There is genetic variability among the accessions of 'Ubá' mango tree evaluated. Therefore, it is possible to make mass selection in open-pollinated populations.

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“…Geneticists and plant breeders are mainly concerned with diversity of germplasm at the molecular level; whereas, horticulturist/pomologist characterize and evaluate the accessions/varieties on the basis of fruit physico-chemical characteristics (Rajwana et al, 2011;Jamil et al, 2015;Naqvi et al, 2015). Previously, genetic diversity has been characterized through morphological traits (Gonzalez et al, 2002;Khan et al, 2015;Azmat et al, 2016) in various fruit crops such as bananas (Gibert et al, 2009), figs (Aljane et al, 2012), papaya (Ocampo et al, 2006), plum (Aazami and Jalili, 2011), mandarins (Domingues, 1999) and cashew nut (Chipojola et al, 2009). However, tree and leave morphological characterization of mango is lacking or still in the initial phase in Pakistan (Rajwana et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

MORPHOLOGICAL AND PHYSICO-CHEMICAL DIVERSITY IN SOME INDIGENOUS MANGO (Mangifera indica L.) GERMPLASM OF PAKISTAN

Raza¹

2017

PAKJAS

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Mango is the second major fruit crop of Pakistan. A large number of unexplored indigenous mango germplasm with great economic significance is present in the country. Hence, 425 mango accessions were studied from Azad Jammu & Kashmir (AJK) as well as Northern and Southern Punjab to explore the genetic diversity. Total 33 traits (25 qualitative and 8 quantitative) enabled the assessment of morphological and physico-chemical diversity of the studied indigenous mango germplasm. The first three principal components (PCs) contributed 68.06% variability among all mango accessions. The PCs also successfully grouped mango accessions according to their morphological and physico-chemical characteristics. Trunk height, tree circumference, crown diameter, leaf colour, leaf blade length, petiole length, inflorescence length, inflorescence width, fruit shape, fruit weight, soluble solid contents, titratable acidity, sugar:acid ratio, reducing sugars, non-reducing sugars and total sugars were found highly variable. Many of these characters are of substantial economic significance and could be used as breeding goals to increase the germplasm repository as well as fruit yield and quality. In conclusion, morphological and physico-chemical traits were highly useful for mango germplasm characterization. Several accessions also showed potentially good traits which could be used to develop new mango cultivars through future breeding schemes.

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DEVELOPMENT OF DNA MARKERS (ISSRs) IN MANGO

Cited by 47 publications

References 0 publications

Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

Genetic Diversity of ‘Ubá’ Mango Tree Using ISSR Markers

MORPHOLOGICAL AND PHYSICO-CHEMICAL DIVERSITY IN SOME INDIGENOUS MANGO (Mangifera indica L.) GERMPLASM OF PAKISTAN

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